Examining gut microbiota and metabolites to clarify mechanisms of Lour leaf components against type 2 diabetes.

Background: Lour leaf components (DLC) contain key active compounds such as quercetin, kaempferol, and quercitrin. They are effective for managing type 2 diabetes mellitus (T2DM), though the exact mechanism by which DLC acts remains unclear.

Aim: To investigate the material basis and mechanism underlying the therapeutic effect of DLC in T2DM.

Comprehensive genomic study of Plasmodium falciparum in Sierra leone: genetic variation and resistance patterns.

Background: Malaria remains a significant global health concern, with being the most dangerous of the malaria-causing parasites. Sierra Leone, with year-round transmission of malaria, continues to experience high infection rates, largely due to . Although genomic databases have provided valuable insights into malaria transmission patterns, drug resistance, and population structure, data from Sierra Leone has been limited.

A self-assembled gold-spiked nanosphere based paper assay for sensitive glucose detection.

Glucose detection plays a crucial role in health monitoring, and the development of efficient and convenient detection platforms is highly desired. This study presents a sensitive and convenient detection system for non-invasive glucose detection in sweat, which is formed by combining the self-assembled gold-spiked nanosphere (GSN) nanozyme with paper-based analytical devices (PADs). The GSN nanozyme exhibits excellent glucose oxidase-like catalytic activity, significantly enhancing the sensitivity and selectivity of glucose detection.

Self-cascade reaction triggered colorimetric detection of choline in triple-negative breast cancer.

Achieving a simple process, detailed information, and visual response for cancer diagnosis and treatment is desirable but challenging. Herein, we develop a self-cascade system-triggered colorimetric array for the sensitive detection of choline, a promising biomarker in triple-negative breast cancer (TNBC). The system utilizes a ChOx-Ce probe, where ChOx catalyzes the oxidation of target choline to betaine, generating HO, which then in situ reacts with Ce to form a CeO nanozyme.

Telitacicept for refractory cystitis associated with severe systemic lupus erythematosus: A case report.

Background: Lupus cystitis, a severe complication of systemic lupus erythematosus (SLE), presents considerable treatment challenges.

Purpose: This case report describes the use of telitacicept in treating severe SLE with lupus cystitis.

Research Design: A single patient with lupus cystitis.

Biogated mesoporous silica nanoagents for inhibition of cell migration and combined cancer therapy.

Migration is an initial step in tumor expansion and metastasis; suppressing cellular migration is beneficial to cancer therapy. Herein, we designed a novel biogated nanoagents that integrated the migration inhibitory factor into the mesoporous silica nanoparticle (MSN) drug delivery nanosystem to realize cell migratory inhibition and synergistic treatment. Antisense oligonucleotides (Anti) of microRNA-330-3p, which is positively related with cancer cell proliferation, migration, invasion, and angiogenesis, not only acted as the locker for blocking drugs but also acted as the inhibitory factor for suppressing migration via gene therapy.

Core-shell "loading-type" nanomaterials enabling glucometer readout for portable and sensitive detection of p-aminophenol in real samples.

A one-target-many-trigger signal model sensing strategy is proposed for quickly, sensitive and on-site detection of the environmental pollutant p-aminophenol (PAP) by use of a commercial personal glucose meter (PGM) for signal readout with the core-shell "loading-type" nanomaterial MSNs@MnO as amplifiable nanoprobes. In this design, the mesoporous silica nanoparticles (MSNs) nanocontainer with entrapped signal molecule glucose is coated with redoxable manganese dioxide (MnO) nanosheets to form the amplifiable nanoprobes (Glu-MSNs@MnO). When encountered with PAP, the redox reaction between the MnO and PAP can induce the degradation of the outer layer of MSNs@MnO, liberating multiple copies of the loaded glucose to light up the PGM signal.

Computational Discovery of High-Temperature Ferromagnetic Semiconductor Monolayer H-MnN.

In the past few years, two-dimensional (2D) high-temperature ferromagnetic semiconductor (FMS) materials with novelty and excellent properties have attracted much attention due to their potential in spintronics applications. In this work, using first-principles calculations, we predict that the H-MnN monolayer with the H-MoS-type structure is a stable intrinsic FMS with an indirect band gap of 0.79 eV and a high Curie temperature () of 380 K.

Self-powered DNA nanomachines for fluorescence detection of lead.

A versatile DNA nanomachine detection system has been developed via the combination of DNAzyme with catalytic hairpin assembly (CHA) technology for achieving accurate and sensitive detection of lead ions (Pb). In the presence of target Pb, capture DNA nanomachine formed by AuNP and DNAzyme recognized and reacted with Pb, which yielded an "active" DNAzyme, that induced the cleavage of substrate strand, and then released the initiator DNA (TT) for CHA. With the help of the initiator DNA TT, self-powered CHA was activated to achieve the signal amplification reaction in the detection of DNA nanomachine.

MnO-DNA nanomaterials toward the dual signal detection of -aminophenol micropollutants.

-Aminophenol (PAP), a potentially toxic and mutagenic compound, is widely distributed in water and soil and has serious side effects on human health. This study presents a convenient, sensitive, and effective dual-signal assay for the detection of PAP in the environment. Two-dimensional manganese dioxide (MnO) nanosheets were used as the carrier and quencher for fluorophore-labelled DNA to form a dual-signal nanoprobe, MnO-DNA.

Core-shell "loading-type" nanomaterials towards: Simultaneous imaging analysis of glutathione and microRNA.

A novel type of core-shell "loading-type" nanomaterials, which integrated excellent biocompatibility, high loading capacity, efficient delivery, dual target recognition and response all-in-one, was fabricated for simultaneous imaging analysis of glutathione and microRNAs in living cells. Specifically, the core-shell "loading-type" nanomaterials (termed as MSNs@MnO) were formed with mesoporous silica nanoparticles (MSNs) as core and a two-dimensional manganese dioxide nanosheets (MnO) as outer layer. Based on the excellent loading capability, the core MSNs was utilized as carriers for signal molecules of rhodamine 6G (R6G).

[Effect of electroacupuncture on pulmonary function and M1 polarization of alveolar macrophages in rats with chronic obstructive pulmonary disease].

Objective: To observe the effect of electroacupuncture (EA) at "Zusanli" (ST36) and "Feishu" (BL13) on M1 polarization of alveolar macrophages (AM) in rats with chronic obstructive pulmonary disease(COPD), so as to explore its anti-inflammatory mechanism underlying improvement of COPD.

Methods: Forty SD rats were randomly divided into normal and normal＋EA, COPD model and COPD＋EA groups (＝10 in each group). The COPD model was established by simple fumigation.

SP1-mediated overexpression of lncRNA LINC01234 as a ceRNA facilitates non-small-cell lung cancer progression via regulating OTUB1.

Long noncoding RNAs (lncRNAs) have been confirmed to be strongly associated with the progression of various types of cancer. LncRNA LINC01234 (LINC01234) is a newly identified tumor-related lncRNA whose upregulation has been confirmed in some tumors. However, its potential expressions and possible functions in non-small-cell lung cancer (NSCLC) have not been explored.

Puerarin alleviates streptozotocin (STZ)-induced osteoporosis in rats through suppressing inflammation and apoptosis via HDAC1/HDAC3 signaling.

Diabetic osteoporosis is a severe public health concern in the world. Puerarin (PU) is extensively attractive due to its superior bioactivities. In the study, we found that PU protected against streptozotocin (STZ)-induced osteoporotic changes in rats.

Effect of dietary zinc pectin oligosaccharides chelate on growth performance, enzyme activities, Zn accumulation, metallothionein concentration, and gene expression of Zn transporters in broiler chickens1.

This study was to investigate the effect of zinc pectin oligosaccharides chelate (Zn-POS) on growth performance, serum enzyme activities, tissue zinc accumulation, metallothionein (MT) concentrations, and gene expression of zinc transporters (ZnT) in broilers. Five hundred forty 1-d-old Arbor Acres broiler chicks were randomly assigned to 5 dietary groups with 6 replicates of 18 birds per replicate. The diets were formulated with the same supplemental Zn level (80 mg/kg diet) but different amount of the Zn-POS: 0, 200, 400, 600, and 800 mg Zn-POS/kg diet.

[Electroacupuncture Improves Pulmonary Function of Rats with Chronic Obstructive Pulmonary Disease by Down-regulating Inflammatory Reaction and Expression of Macrophage Migration Inhibitory Factor/CD 74-CD 44/p 38 MAPK Signaling in Lung Tissues].

Objective: To observe the effect of electroacupuncture (EA) at "Zusanli" (ST 36) and "Feishu" (BL 13) on pulmonary function, inflammatory reaction and expression of macrophage migration inhibitory factor (MIF) and its receptor complex CD 74-CD 44, etc. in rats with chronic obstructive pulmonary disease (COPD), so as to explore its mechanism underlying improvement of COPD.

Methods: Thirty male SD rats were randomly divided into normal, model and EA groups (＝10 in each group).

Divergent Evolutional Mode and Purifying Selection of the Gene in European and Asian Domestic Pig Breeds.

The recent geographic expansion of wild boars and the even more recent development of numerous domestic pigs have spurred exploration on pig domestic origins. The porcine gene has been showed to affect pleiotropic effects, blood parameters, and coat colour phenotypes, especially the white colour phenotype formation in European commercial breeds. Here, we described the use of SNPs to identify different selection patterns on the porcine gene and the phylogenetic relationships of the inferred haplotypes.

[Effects of α-pinene extracted from pine needle on expression of miR-221 and its potential target genes in human hepatocellular carcinoma cells].

To investigate the anti-hepatoma mechanism of α-pinene, HepG2 cell was treated with α-pinene and the change of cell cycle was examined by flow cytometry. The expression of miR-221, which was related the regulation of G₂/M phase, was detected by quantitative Real-time PCR. Meanwhile, TargetScan and other online bioinformatics methods were used to analyze and predict the target genes of miR-221, then the expression level of related target genes were detected by quantitative Real-time PCR.

The effect of n-3/n-6 polyunsaturated fatty acids on acute reflux esophagitis in rats.

Background: Polyunsaturated fatty acids (PUFAs) play various roles in inflammation. However, the effect of PUFAs in the development of reflux esophagitis (RE) is unclear. This study is to investigate the potential effect of n-3/n-6 PUFAs on acute RE in rats along with the underlying protective mechanisms.

Decreased n-6/n-3 polyunsaturated fatty acid ratio reduces chronic reflux esophagitis in rats.

Aim: To investigate the effect of dietary ratio of n-6/n-3 PUFAs on chronic reflux esophagitis (RE) and lipid peroxidation.

Method: Rat RE model were established and then fed on a diet contained different n-6/n-3 PUFA ratios (1:1.5, 5:1, 10:1) or received pure n-6 PUFA diet for 14 days.

Ex vivo expansion of hematopoietic stem cells.

Ex vivo expansion of hematopoietic stem cells (HSCs) would benefit clinical applications in several aspects, to improve patient survival, utilize cord blood stem cells for adult applications, and selectively propagate stem cell populations after genetic manipulation. In this review we summarize and discuss recent advances in the culture systems of mouse and human HSCs, which include stroma/HSC co-culture, continuous perfusion and fed-batch cultures, and those supplemented with extrinsic ligands, membrane transportable transcription factors, complement components, protein modification enzymes, metabolites, or small molecule chemicals. Some of the expansion systems have been tested in clinical trials.