Survival outcomes and risk factors after lung transplantation in patients with pre-transplant malignancy: a national cohort study.

Background: Pre-transplant malignancy (PTM) is a relative contraindication for lung transplantation (LTx). The proportion of patients with PTM in LTx is increasing annually. We sought to identify modifiable risk factors affecting patient survival and evaluate the prognosis of different types of PTM.

Association between iron homeostasis and prognosis in patients with chronic obstructive pulmonary disease: a retrospective analysis from MIMIC-IV database.

Introduction: Accumulating evidence indicates that inflammatory responses can alter iron-related biomarkers, such as serum iron, ferritin, transferrin, and total iron-binding capacity (TIBC). However, in the context of chronic obstructive pulmonary disease (COPD), characterized by airway inflammation, the relationship between its prognosis and iron-related biomarkers has not been comprehensively assessed.

Methods: Clinical data of 611 COPD patients from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database were retrospectively analyzed.

Type 2 Diabetes Impacts the Efficacy of Syphilis Treatment and is a Risk Factor for Late Syphilis: A Retrospective Cohort Study.

This study examined the relationship between type 2 diabetes and late syphilis. A retrospective analysis was conducted, including 96 syphilis patients with type 2 diabetes and 122 matched non-diabetic syphilis controls. The development of late-stage syphilis and responses to initial treatment were monitored over a 2-year period.

Investigating allergen-specific IgE distribution and correlations in chronic urticaria: a retrospective study in Shanghai, China.

Background: Chronic urticaria (CU) is a persistent skin condition characterized by recurring episodes triggered by diverse allergens and a multifaceted causative nature. The present study aimed to retrospectively explore the distribution patterns and correlations of specific IgE (sIgE) antibodies with various allergens among patients with CU.

Methods: For this purpose, the present study enrolled 820 patients with CU treated at Shanghai Skin Disease Hospital from July 2020 to September 2023.

Spontaneous ventilation video-assisted thoracoscopic surgery for octogenarian non-small cell lung cancer patients: a non-inferiority study.

Background: The benefits of spontaneous ventilation (SV)-video-assisted thoracoscopic surgery (VATS) in octogenarian patients with non-small-cell lung cancer (NSCLC) have rarely been reported. This retrospective study was conducted to evaluate the safety and feasibility of SV-VATS in octogenarian patients with NSCLC.

Methods: Patients with NSCLC aged >80 years who underwent SV-VATS or mechanical ventilation (MV)-VATS between 2017 and 2022 were included in this study.

Survival and early outcomes following lung transplantation for interstitial lung disease associated with non-scleroderma connective tissue disease: a national cohort study.

Objectives: The progressive decline in interstitial lung disease associated with non-scleroderma connective tissue disease (ILD-NSCTD) is linked to poor prognosis and frequently results in respiratory failure. Lung transplantation (LTx) offers a viable treatment option, yet its outcomes in ILD-NSCTD remain contentious, particularly across different subtypes.

Methods: This retrospective cohort study included patients with idiopathic pulmonary fibrosis (IPF) (n=11,610) and ILD-NSCTD (n=610) listed in the United Network for Organ Sharing (UNOS) database who underwent lung transplantation between May 5, 2005, and December 31, 2022.

Neutrophil-related indicators as potential peripheral blood biomarkers for the diagnosis of neurosyphilis: a cross-sectional study.

Background: Neurosyphilis (NS) is the most serious complication elicited by the invasion of Treponema pallidum (T. pallidum) into the central nervous system. Identifying the neuro-invasion of T.

Lobar lung transplantation in the mouse.

Background: As an important supplementary approach to clinical in orthotopic lung transplantation (LTx), lobar LTx currently lacks a stable animal model and in the orthotopic left LTx model, the right lung of the donor mouse is completely removed and discarded. We introduce a novel mouse lobar LTx model that potentially provides a mouse model for clinical lobar LTx and increase the utilization rate of the experimental donor.

Methods: Lobar and orthotopic left LTx were performed in syngeneic strain combinations.

Risk factors for mid- and long-term mortality in lung transplant recipients aged 70 years and older.

Objectives: With increased lung transplantation in those aged 70 and older, limited literature addresses risk factors affecting their survival. Our study aims to identify independent factors impacting mid- and long-term mortality in this elderly population.

Methods: This study analyzed lung transplant patients over 70 from May 2005 to December 2022 using United Network for Organ Sharing data.

Immunophenotypic variations in syphilis: insights from Mendelian randomization analysis.

Background: Infection with instigates complex immune responses. Prior research has suggested that persistent infection can manipulate host immune responses and circumvent host defenses. However, the precise role of immune cells in infection across different stages remains a contentious issue.

Neutrophil CD64 index as a potential blood biomarker for the diagnosis of neurosyphilis in secondary and tertiary syphilis: A retrospective study.

Objective: To examine the correlation of neutrophil CD64 (nCD64) index with neurosyphilis (NS) across different stages of syphilis.

Methods: A total of 1243 syphilis patients at different stages (344 of primary, 385 of secondary, and 514 of tertiary) included in this study were divided into NS and non-NS (NNS). Correlations of nCD64 index with currently used syphilis biomarkers were explored using Spearman correlation test.

Epidemiological characteristics and influencing factors of acute leukemia in children and adolescents and adults: a large population-based study.

Objective: To assess the epidemiological characteristics and prognostic factors of acute leukemia (AL) in children and adolescents, and make comparisons between pediatric and adult patients.

Methods: This retrospective cohort study enrolled AL patients from the Surveillance, Epidemiology, and End Results (SEER) 1975-2016. OS in children and adolescents and adults with AL was compared and analyzed separately by age and AL subtype.

The W-Acidic Motif of Histidine Kinase WalK Is Required for Signaling and Transcriptional Regulation in .

In , we find that the histidine kinase WalK possesses the longest C-terminal tail (CTT) among all 14 TCSs, and this tail plays a key role in the interaction of WalK with its response regulator WalR. We demonstrate that the intrinsically disordered CTT is characterized by a conserved tryptophan residue surrounded by acidic amino acids. Mutation in the tryptophan not only disrupts the stable interaction, but also impairs the efficient phosphotransferase and phosphatase activities of WalRK.

Role of rgsA in Oxidative Stress Resistance in Pseudomonas aeruginosa.

Pseudomonas aeruginosa is a common opportunistic pathogen that causes infections in vulnerable patients including those with metabolic disorders, hematologic diseases, and malignancies, and in those who have undergone surgery. In addition, P. aeruginosa exhibits high intrinsic resistance to numerous antibiotics and tends to form biofilms rendering it even more refractory to treatment.

Diagnostic Value of Vaginal Microecology, Serum miR-18a, and PD-L1 for Identifying HPV-Positive Cervical Cancer.

Objective: We aimed to investigate the diagnostic value of the vaginal microecology, serum miR-18a, and programmed death ligand-1 (PD-L1) for human papillomavirus (HPV)-positive cervical cancer.

Methods: Eighty-four patients with HPV-positive cervical cancer were assigned to the observation group, 107 HPV-positive patients without cervical cancer were assigned to the positive group, and 191 healthy women were assigned to the control group. Vaginal microecology and serum levels of miR-18a and PD-L1 on the surface of CD4 and CD8 T cells were compared among the 3 groups.

Long noncoding RNA THAP9-AS1 is induced by and promotes cell growth and migration of gastric cancer.

Background: Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and () is the major risk factor for GC. In this study, we focused on the roles of -related lncRNAs in the progression of GC.

Diagnostic accuracy of γ-glutamyl transpeptidase-to-platelet ratio for predicting hepatitis B-related fibrosis: a meta-analysis.

Background And Aim: Emerging published data on the accuracy of γ-glutamyl transpeptidase-to-platelet ratio (GPR) for diagnosing hepatitis B virus (HBV)-related fibrosis are inconsistent. The aim of this study was to systematically review the performance of GPR for diagnosing HBV-related significant fibrosis, severe fibrosis, and cirrhosis.

Patients And Methods: A comprehensive literature search of PubMed, Web of Science, and EMBASE was conducted before July 2018.

Conditioned medium mimicking the tumor microenvironment augments chemotherapeutic resistance via ataxia‑telangiectasia mutated and nuclear factor‑κB pathways in gastric cancer cells.

The tumor microenvironment affects the processes involved in the development of gastric cancer and contributes to multidrug resistance (MDR). Although the metabolism of gastric cancer cells is known to be associated with the development of the tumor microenvironment, the exact role of metabolism in microenvironment‑induced MDR formation remains unclear. In the present study, conditioned medium (CM) formed through the metabolism of SGC‑7901 gastric carcinoma cells was used to mimic the tumor microenvironment.

Molecular characterization of multidrug-resistant Klebsiella pneumoniae isolates.

Klebsiella pneumoniae is an important cause of healthcare-associated infections worldwide. Selective pressure, the extensive use of antibiotics, and the conjugational transmission of antibiotic resistance genes across bacterial species and genera facilitate the emergence of multidrug-resistant (MDR) K. pneumoniae.

ClpP affects biofilm formation of Streptococcus mutans differently in the presence of cariogenic carbohydrates through regulating gtfBC and ftf.

The abilities to form biofilms on teeth surface and to metabolize a wide range of carbohydrates are key virulence attributes of Streptococcus mutans. ClpP has been proved to play an important role in biofilm development in streptococci. Here we demonstrated that ClpP was involved in biofilm formation of S.

Heptaplex PCR melting curve analysis for rapid detection of plasmid-mediated AmpC β-lactamase genes.

Antimicrobial resistance mediated by plasmid-borne AmpC β-lactamase in Gram-negative bacteria is an emerging event of significant clinical importance. Rapid and reliable detection of ampC is in urgent need for appropriate infection control. We described the development and evaluation of a heptaplex PCR melting curve analysis that could identify six groups of ampC, i.

Contribution of ClpP to stress tolerance and virulence properties of Streptococcus mutans.

Abilities to tolerate environmental stresses and to form biofilms on teeth surface are key virulence attributes of Streptococcus mutans, the primary causative agent of human dental caries. ClpP, the chief intracellular protease of S. mutans, along with ATPases degrades altered proteins that might be toxic for bacteria, and thus plays important roles in stress response.

3'-Phosphoadenosine-5'-phosphate phosphatase activity is required for superoxide stress tolerance in Streptococcus mutans.

Aerobic microorganisms have evolved different strategies to withstand environmental oxidative stresses generated by various reactive oxygen species (ROS). For the facultative anaerobic human oral pathogen Streptococcus mutans, the mechanisms used to protect against ROS are not fully understood, since it does not possess catalase, an enzyme that degrades hydrogen peroxide. In order to elucidate the genes that are essential for superoxide stress response, methyl viologen (MV)-sensitive mutants of S.

A phenotypic microarray analysis of a Streptococcus mutans liaS mutant.

Streptococcus mutans, a biofilm-forming Gram-positive bacterium that resides in the human oral cavity, is considered to be the primary aetiological agent of human dental caries. A cell-envelope stress-sensing histidine kinase, LiaS, is considered to be important for expression of virulence factors such as glucan-binding protein C and mutacin production. In this study, a liaS mutant was subjected to phenotypic microarray (PM) analysis of about 2000 phenotypes, including utilization of various carbon, nitrogen, phosphate and sulfur sources; osmolytes; metabolic inhibitors; and susceptibility to toxic compounds, including several types of antibiotics.