Disruption of egg and nymph development via RNAi-mediated Glutamine: fructose-6-phosphate aminotransferase knockdown in Locusta migratoria: A promising strategy for pest management.

Glutamine: fructose-6-phosphate aminotransferase (GFAT) is the first rate-limiting enzyme in the hexosamine biosynthetic pathway, which plays a crucial role in various biological processes, including chitin metabolism in insects. Locusta migratoria, a widespread and highly destructive agricultural pest, poses a significant threat due to its rapid reproduction and long-distance migration. In this study, we identified and characterized LmGFAT as a key regulator of locust development.

Sowing Methods and Strigolactones Alleviate Damage to the Photosynthetic System of Rice Seedlings Under Salt Stress by Enhancing Antioxidant Capacity.

Seedling cultivation of rice ( L.) is a critical initial step in rice production. This study investigated the effects of sowing methods and strigolactone (GR24) on rice seedlings under salt stress.

Case Report: Refractory pneumonia complicated by pulmonary embolism and infarction in a child.

(MP) is a significant pathogen of community-acquired pneumonia in children, typically following a benign course. However, some cases may progress to severe or refractory MP pneumonia (SMPP or RMPP) and lead to thromboembolic complications. This report describes a rare case of a 9-year-old boy with RMPP complicated by bilateral pulmonary embolism (PE) and pulmonary infarction.

Alpha-Linolenic Acid from Seed Powder Regulates Fatty Acid Metabolism and Influences Meat Quality of Pekin Duck via the ADIPOQ/AMPK/CPT-1 Pathway.

seed powder contains dominant α-linolenic acid (ALA). Its regulatory mechanism as a novel feed additive for the livestock field is not clear. In this study, RNA-seq was used to identify the differential gene expression in breast muscle of Pekin duck supplemented with different doses of seed powder, and was found to be an important factor.

In-depth analysis of cystic fibrosis cases caused by gene variation and research on the prediction and simulation of the impact on protein function.

Background: Cystic fibrosis (CF) is caused by gene mutations. Its diagnosis mainly depends on genetic and sweat chloride tests, but the complexity of these mutations challenges diagnosis.

Methods: This paper reports a new case of a Chinese child with cough and wheezing, suspected of having CF.

Chitosan nanoparticle-mediated delivery of dsRNA for enhancing RNAi efficiency in Locusta migratoria.

Background: RNA interference (RNAi) targeting the key genes involved in insect growth and development has been demonstrated as pollution-free green pest management strategies. Double-stranded RNA (dsRNA)-based biopesticides offer species-specific pest control and degrade rapidly in the environment, making them a safer alternative to conventional chemical pesticides. However, efficient delivery of dsRNA is a significant challenge in the application of RNAi technology for pest management.

Rab GTPases are evolutionarily conserved signals mediating selective autophagy.

Selective autophagy plays a crucial role in maintaining cellular homeostasis by specifically targeting unwanted cargo labeled with "autophagy cues" signals for autophagic degradation. In this study, we identify Rab GTPases as a class of such autophagy cues signals involved in selective autophagy. Through biochemical and imaging screens, we reveal that human Rab GTPases are common autophagy substrates.

Integrative analysis of transcriptome and metabolome reveal molecular mechanism of tolerance to salt stress in rice.

Background: Salt stress is considered to be one of the major abiotic stresses influencing rice growth and productivity. To improve rice crop productivity in saline soils, it is essential to choose a suitable variety for mitigating salt stress and gain a deep understanding of the underlying mechanisms. The current study explored the salt tolerance mechanism of wild rice 'HD96-1 (salt resistive)' and conventional rice 'IR29 (salt sensitive)' by evaluating morph-physiological, transcriptomic, and metabolomic approaches.

PEI-SWNT improves RNAi efficiency in Locusta migratoria via dsRNA injection delivery system.

The instability of double-stranded RNA (dsRNA) restricts the application of RNA interference (RNAi) technology in agricultural pest management. Various types of nanocarriers have been developed and employed for the stable delivery of dsRNA. Nonetheless, it remains unclear which type of nanomaterial could deliver dsRNA stably and efficiently for gene knockdown in Locusta migratoria.

-Mediated Insulin Signaling Pathway Was Involved in O-Induced Multigenerational Effects of Shortened Lifespan in .

As a long-standing atmospheric pollutant, ozone (O) exerts enduring effects on biological health. However, experimental research on its impact on organism lifespan and generational effects is limited. This study exposed three generations of fruit flies () to O, revealing a shortened lifespan across generations.

Prohexadione Calcium Improves Rice Yield Under Salt Stress by Regulating Source-Sink Relationships During the Filling Period.

Salt stress is an important factor affecting the growth and development of rice, and prohexadione calcium (Pro-Ca) plays an important role in alleviating rice salt stress and improving rice yield. However, there are few studies on how Pro-Ca improves rice yield under salt stress by regulating the source-sink metabolism. In this study, we used Guanghong 3 (salt-tolerant variety) and Huanghuazhan (salt-sensitive variety) as experimental materials to investigate the dynamic changes in the synthesis and partitioning of nonstructural carbohydrates among source-sink, the dynamic changes in related enzyme activities, the effects of the source-sink metabolism on yield in rice under salt stress and the effect of Pro-Ca during the filling period.

Regulation of Exogenous Strigolactone on Storage Substance Metabolism and Endogenous Hormone Levels in the Early Germination Stage of Rice Seeds Under Salt Stress.

Salt stress inhibits rice seed germination. Strigolactone (GR24) plays a vital role in enhancing plant tolerance against salt stress. However, GR24's impact on the metabolism of stored substances and endogenous hormones remains unclear.

Mitigation Effect of Exogenous Nano-Silicon on Salt Stress Damage of Rice Seedlings.

Salt stress represents a significant abiotic stress factor that impedes the growth of rice. Nano-silicon has the potential to enhance rice growth and salt tolerance. In this experiment, the rice variety 9311 was employed as the test material to simulate salt stress via hydroponics, with the objective of investigating the mitigation effect of foliar application of nano-silicon on rice seedlings.

Effects of adding a kind of compound bio-enzyme to the diet on the production performance, serum immunity, and intestinal health of Pekin ducks.

The use of bio-enzyme as feed additives holds significant potential. This study aimed to evaluate the impact of a kind of compound bio-enzyme supplementation (the main functional components are probiotics and astragalus polysaccharides) on the production performance, serum immunity, and intestinal health of Pekin ducks. A total of 126 male Pekin ducks were randomly assigned to three groups: a control group (CG, no additive), a low-dose group (LG, 0.

Free-caged rearing modes regulate chicken intestinal metabolism by influencing gut microbial homeostasis.

Free-caged rearing modes, which prioritize animal welfare, are believed to enhance the quality of animal products. The impact of rearing modes on meat quality may play a key role in the superior quality of local chicken breeds. This study analyzed the cecal contents of free-range and caged black-bone chickens at different ages using metagenomic and metabolomic sequencing.

Ozone induced multigenerational glucose and lipid metabolism disorders in Drosophila.

Ozone (O), a persistent pollutant, poses a significant health threat. However, research on its multigenerational toxicity remains limited. Leveraging the Drosophila model with its short lifespan and advanced genetic tools, we explored the effects of O exposure across three generations of fruit flies.

Identification and Expression Analysis of Cytochrome P450 Genes Probably Involved in Triterpenoid Saponins Biosynthesis in .

Cytochromes P450 (P450s) are one of the largest enzymatic protein families and play critical roles in the synthesis and metabolism of plant secondary metabolites. Astragaloside IV (AS-IV) is one of the primary active components in herbs, exhibiting diverse biological activities and pharmacological effects. However, P450s involved in the astragaloside biosynthesis have not been systematically analyzed in ().

Elevated LDL-c may warn of the risk of gallbladder stones in the patients with metabolic dysfunction-associated steatotic liver disease: A case-control study.

Purpose: This study aimed to explore the relationship between metabolic dysfunction-associated steatotic liver disease (MASLD) and the development of new-onset gallbladder stone disease (GSD) and to identify factors that influence the occurrence of new-onset GSD in patients with MASLD.

Methods: In this retrospective case-control study, patients who underwent asymptomatic GSD screening during annual routine health check-ups at two hospitals in China between August 2017 and July 2022 were included. Patients with new-onset GSD and controls without GSD were matched 1:1 based on age, sex, race, occupation, diet, drinking habits, systolic blood pressure, diastolic blood pressure, and fasting blood glucose levels.

Combining Low-Light Scene Enhancement for Fast and Accurate Lane Detection.

Lane detection is a crucial task in the field of autonomous driving, as it enables vehicles to safely navigate on the road by interpreting the high-level semantics of traffic signs. Unfortunately, lane detection is a challenging problem due to factors such as low-light conditions, occlusions, and lane line blurring. These factors increase the perplexity and indeterminacy of the lane features, making them hard to distinguish and segment.

Gasdermin D affects aortic vascular smooth muscle cell pyroptosis and Ang II-induced vascular remodeling.

Vascular smooth muscle cells (VSMCs) are primarily responsible for vasoconstriction and the regulation of blood pressure1. Pyroptosis, a particular form of regulated cell death, is involved in multiple vascular injuries, including hypertensive vascular dysfunction. This pyroptotic cell death is mediated by the pore-forming protein of Gasdermin D (GSDMD).

The inverse association between fasting blood glucose and the occurrence of gallbladder cancer in type 2 diabetes mellitus patients: a case-control study.

Objective: This study aimed to explore the correlation between diabetes mellitus (DM) and gallbladder cancer (GBC) in an epidemiological setting.

Methods: The study summarized the clinical and laboratory data of 2210 GBC Chinese patients in the authors' hospital. A total of 17 influencing factors for GBC, including gender, body mass index (BMI), fasting blood glucose (FBG), fasting insulin (FINS), the homeostasis model assessment of insulin resistance (HOMA-IR), retinol-binding protein 4 (RBP4), and lipid indexes were analyzed using unconditional logistic regression analysis.

Effect of pneumovesicoscopic cohen surgery with adjustable suspension technique through the urethra on the treatment of primary vesicoureteral reflux disease in infants.

Objective: The objective of this study was to evaluate the safety and efficacy of pneumovesicoscopic Cohen surgery with an adjustable suspension technique through the urethra for the treatment of primary vesicoureteral reflux disease in infants.

Methods: This study retrospectively analysed the clinical data of 31 infants who underwent pneumovesicoscopic Cohen surgery with an adjustable suspension technique through the urethra in our hospital from January 2019 to December 2020. We also collected the clinical data of 29 infants who underwent open Cohen surgery in our hospital from January 2015 to December 2018 as a control variable.