Role of lncRNA NONMMUT104330.1-miR-709-DDX58 axis in mediating immunomodulatory effects of enoxaparin sodium bone cement on macrophage polarization and inflammatory response.

Background: PMMA bone cement is known to induce tissue inflammation around implants. A previous research group developed a novel material, enoxaparin sodium bone cement (ES-PMMA), which combines 40 g of PMMA bone cement with 8000 AxaIU of enoxaparin sodium. This innovative bone cement has demonstrated anti-inflammatory and immunomodulatory properties within the local tissue microenvironment.

A telomere-to-telomere gap-free genome assembly of the endangered humphead wrasse (Cheilinus undulatus).

Humphead wrasse, Cheilinus undulatus, is an endangered fish species with high economic and ecological value as well as natural sex change from female to male, while sexual selection occurs in breeding aggregations. In our present study, we constructed the first gap-free telomere-to-telomere (T2T) genome assembly for humphead wrasse, by integration of PacBio HiFi, ONT Ultra-long and Hi-C sequencing techniques. With 99% of the entire sequences anchored into 24 chromosomes, this haplotypic genome assembly spans approximately 1.

Biomechanical impact of enoxaparin sodium-chitosan-PMMA bone cement in hip arthroplasty: a preliminary finite element analysis.

Objective: To determine the material parameters of PMMA and ES-CS-PMMA, and compare the stress distribution differences in hip arthroplasty postoperatively using finite element analysis.

Methods: The Young's modulus and Poisson's ratio of PMMA and ES-CS-PMMA were calculated by measuring the propagation speed of ultrasound waves through the bone cements. Using CT images of a healthy adult male, a three-dimensional model of the natural femur and two postoperative 3D models (with PMMA and ES-CS-PMMA as adhesives) were established.

Genome-Wide Identification of DNA Methyltransferases (Dnmts) in Fish and Its Potential Roles During Sex Change in Blackhead Seabream.

DNA methylation, also known as 5-methylcytosine, is an epigenetic modification that has crucial functions in multiple important biological processes in fish, such as gonadal development. The cellular DNA methylation level is tightly regulated by DNA methyltransferases (Dnmt). However, detailed investigations of this family in fish are very scarce.

EccDNA Analysis Provides Novel Insights Into the Molecular Mechanism of Firmness of Fish Fillet.

Extrachromosomal circular DNAs (eccDNAs) play a significant role in regulating various biological processes, including abnormal muscle development. The molecular functions and impact of eccDNAs in the muscle development of fish are poorly understood. To investigate the potential roles of eccDNAs in the muscle development of fish, we analyzed and compared the expression profile of muscle eccDNAs of crisp grass carp, fed a faba bean meal-based diet, and ordinary grass carp, fed a practical diet.

Integrative DNA methylome and transcriptome analysis identify potential genes on the influence of dilated cardiomyopathy-associated heart failure.

Objective: Dilated cardiomyopathy (DCM)-associated heart failure (HF) presents a significant clinical challenge, underlying epigenetic mechanisms remaining poorly understood. This study aims to investigate the interplay between DNA methylation and gene expression in the hearts of patients with DCM-associated HF (DCM-HF).

Methods: Atrial tissues were collected from five healthy donors and five heart transplant recipients suffering from heart failure due to DCM.

Engineered S. cerevisiae construction for high-gravity ethanol production and targeted metabolomics.

Strong sugar tolerance and high bioethanol yield of yeast under high-gravity fermentation have caused great attention in the bioethanol industry. In this study, Clustered Regularly Interspaced Short Palindromic Repeats Cas9 (CRISPR-Cas9) technology was used to knock out S. cerevisiae GPD2, FPS1, ADH2, DLD3, ERG5, NTH1, and AMS1 to construct engineering strain S.

Prevalence and Risk Factors of Lower Extremity Musculoskeletal Disorders Among Occupational Groups in Key Industries - China, 2018-2023.

What Is Already Known About This Topic?: Lower extremity musculoskeletal diseases (LE-MSDs) have emerged as a significant contributor to the global disease economic burden and worker absenteeism, becoming a global public health concern. However, the epidemic characteristics of LE-MSDs among occupational populations in China are unknown.

What Is Added By This Report?: This report finds that the LE-MSDs prevalence rate among key occupational groups in China is 17.

construction enhancing stress tolerance for ethanol production increase in the presence of inhibitors and mechanism analysis based on the comparative transcriptomics.

is considered the most promising large-scale production strain with ethanol as the main product. The fermentation of is generally inhibited under various stress conditions. Various inhibitors in the hydrolysate severely inhibit yeast proliferation and yeast accumulation.

SMART: STR Mixture Analysis and Resolution Tools.

The analysis of STR mixture profiles derived from mixed DNA samples plays a critical role in criminal investigations and legal proceedings. In this article, we present SMART, a novel software developed within the fully continuous model framework to analyze STR mixture profiles. SMART incorporates the peak height model, stutter model, drop-in/drop-out model, and population genetics model, offering various functionalities such as calculating likelihood ratios (LR), resolving genotypes of individual contributors, and performing direct database searches using mixed DNA profiles.

Effect of Si and Holding Time on TiAlLa Phase in Al-Ti-La Intermediate Alloy.

The effects of holding time and Si on the content, shape size and structure of TiAlLa phase in Al-Ti-La intermediate alloy were investigated by an X-ray diffractometer, scanning electron microscope and transmission electron microscope. The results show that the volume fraction and aspect ratio of TiAlLa phase in Al-Ti-La intermediate alloy decrease significantly, from 21% and 2.3 without Si addition to 4% and 2.

Risk and related factors of elbow musculoskeletal diseases: a nationwide cross-sectional survey in China.

Objectives: Although studies have shown that work-related musculoskeletal disorders (WMSDs) are common and continue to be a major source of disability and work time loss, there are few reports about elbow WMSDs. The aim of this study was to explore the prevalence and associated factors of elbow WMSDs.

Methods: The valid questionnaires of 57501 workers from 15 different industries nationwide were collected and the c2 test and logistic regression analysis were applied to reveal the prevalence and risk factors of elbow WMSDs.

Emergent Chirality and Hyperuniformity in an Active Mixture with Nonreciprocal Interactions.

We investigate collective dynamics in a binary mixture of programmable robots in experiments and simulations. While robots of the same species align their motion direction, interaction between species is distinctly nonreciprocal: species A aligns with B and species B antialigns with A. This nonreciprocal interaction gives rise to the emergence of collective chiral motion that can be stabilized by limiting the robot angular speed to be below a threshold.

Immobilization of recombinant Trametes versicolor aflatoxin B-degrading enzyme (TV-AFBD) with montmorillonite for absorption and in situ degradation of aflatoxin B.

Aflatoxin B is a highly carcinogenic and teratogenic substance mainly produced by toxin-producing strains such as Aspergillus flavus and Aspergillus parasitic. The efficient decomposition of aflatoxin is an important means to reduce its harm to humans and livestock. In this study, Trametes versicolor aflatoxin B-degrading enzyme (TV-AFBD) was recombinantly expressed in Bacillus subtilis (B.

Robust All-Solid-State Lithium Metal Batteries Enabled by a Composite Lithium Anode with Improved Bulk Li Diffusion Kinetics Properties.

All-solid-state batteries (ASSBs) with a Li metal anode are expected to be one of the most promising energy storage systems to achieve high energy density. However, the interfacial instability between the Li metal anode and solid-state electrolyte (SSE) limits the rate capability and cycling stability of ASSBs. The main issue is the formation of voids at the Li/SSE interface during Li stripping due to the slow diffusion of Li within the bulk Li metal, then increasing internal cell resistance and inducing the formation of lithium dendrites.

Exploring mRNA translation strategies for hypoxia adaptation across distantly related metazoans.

Adaptation to variations in oxygen concentration is a conserved mechanism in all metazoans. Extensive studies have been focused on the roles of hypoxia-inducible factors (HIFs) in response to hypoxia. However, HIF1α is not conserved in all animals, and HIF2α and HIF3α are only observed in vertebrates.

Enoxaparin sodium bone cement plays an anti-inflammatory immunomodulatory role by inducing the polarization of M2 macrophages.

Objective: The implantation of PMMA bone cement results in an immune response and the release of PMMA bone cement particles causes an inflammatory cascade. Our study discovered that ES-PMMA bone cement can induce M2 polarization of macrophages, which has an anti-inflammatory immunomodulatory effect. We also delved into the molecular mechanisms that underlie this process.

Thermotolerance improvement of engineered Saccharomyces cerevisiae ERG5 Delta ERG4 Delta ERG3 Delta, molecular mechanism, and its application in corn ethanol production.

Background: The thermotolerant yeast is beneficial in terms of efficiency improvement of processes and reduction of costs, while Saccharomyces cerevisiae does not efficiently grow and ferment at high-temperature conditions. The sterol composition alteration from ergosterol to fecosterol in the cell membrane of S. cerevisiae affects the thermotolerant capability.

Incidence and Risk Factors of the Upper-Limb Musculoskeletal Disorders Among Occupational Groups in Key Industries - China, 2018-2021.

What Is Already Known About This Topic?: The burden of illness and economic losses due to upper-limb work-related musculoskeletal disorders (UL-WMSDs) is high; thus, they have become a major global public health problem. At present, the epidemiological characteristics of UL-WMSDs in China's occupational population are still unknown.

What Is Added By This Report?: The incidence of UL-WMSDs among key occupational groups in China is 22.

Hexaindium Heptasulfide/Nitrogen and Sulfur Co-Doped Carbon Hollow Microspindles with Ultrahigh-Rate Sodium Storage through Stable Conversion and Alloying Reactions.

Group IIIA-VA metal sulfides (GMSs) have attracted increasing attention because of their unique Na-storage mechanisms through combined conversion and alloying reactions, thus delivering large theoretical capacities and low working potentials. However, Na diffusion within GMSs anodes leads to severe volume change, generally representing a fundamental limitation to rate capability and cycling stability. Here, monodispersed In S /nitrogen and sulfur co-doped carbon hollow microspindles (In S /NSC HMS) are produced by morphology-preserved thermal sulfurization of spindle-like and porous indium-based metal organic frameworks.

Epidemiological study of multi-site WMSDs in the footwear industry in China.

. This study aimed to identify the prevalence of multi-site work-related musculoskeletal disorders (WMSDs) in Chinese footwear industry workers from 2018 to 2020 and explore the possible influencing factors of WMSDs. .

Prevalence status and associated factors of wrist postural injury in the Chinese occupational population.

Objective: This study investigated the prevalence of wrist injuries in 15 industries and different types of work in China. Study on the associated factors of wrist injuries provides a scientific basis for prevention and treatment of wrist diseases in occupational workers.

Methods: A cross-sectional study of musculoskeletal symptoms of related practitioners in 15 industries, including automobile manufacturing, was conducted to retrieve worker demographic information, working wrist posture, and pain conditions.

Shoulder work-related musculoskeletal disorders and related factors of workers in 15 industries of China: a cross-sectional study.

Background: Changes in modern industrial production practices can easily lead to shoulder work-related musculoskeletal disorders (WMSD). The current reports on shoulder WMSD are limited to some industries are less well studied, and the sample size is usually small. This study aimed to describe the prevalence and severity of shoulder WMSD in a large sample of Chinese workers from 15 industries, analyze the possible correlations with sociodemographic and work-related variables, and compare the differences between industries.

Characterization of a aflatoxin B1-degrading enzyme (TV-AFB1D) and its application in the AFB1 degradation of contaminated rice .

Aflatoxin B (AFB) contaminates rice during harvest or storage and causes a considerable risk to human and animal health. In this study, AFB-degrading enzyme (TV-AFBD) gene recombinantly expressed in engineered BL21 (DE3) and . The TV-AFBD enzymatic characteristics and AFB degradation efficiency in contaminated rice were investigated.