Ablations of Human and Murine TNK2/ACK1 Proteins Do Not Alter Influenza A Virus Infections In Vitro or In Vivo.

Tyrosine kinase non-receptor2 (TNK2) is a host protein involved in vesicular trafficking, cell spreading, migration, survival, and proliferation. TNK2 has been identified as a conserved host factor for the entry of several non-enveloped RNA viruses, such as Orsay virus in Caenorhabditis elegans and multiple picornaviruses in cells and mice. Although TNK2 was reported as required for influenza A virus infection in a genome-wide CRISPR screen, its role remains contentious as it was not identified in other screens.

Audio-visual crossmodal connectivity in perceiving distorted speech: A comparison between patients with cleft palate and typical listeners.

This study investigates audio-visual crossmodal connectivity during the perception of distorted speech using task-based functional magnetic resonance imaging (fMRI). A randomized block design was employed, involving 20 patients with cleft palate and 20 typical listeners. Participants underwent perceptual tasks involving both cleft-related glottal stop and typical speech while fMRI scans were conducted.

Comprehensive 3D measurements of condyle changes in patients with class III asymmetry after bimaxillary orthognathic surgery.

Objectives: The present study was aimed to assess three-dimensional (3D) quantitative changes of condyle in patients with Class III facial asymmetry after bimaxillary orthognathic surgery.

Materials And Methods: Fifty-five eligible patients with Class III facial asymmetry received Le Fort I osteotomy and bilateral sagittal split osteotomy (BSSO) with or without genioplasty from January 2020 to June 2022 were included. Cone-beam computed tomography (CBCT) for each patient was collected at 3 stages: 1 week preoperatively (T0), immediately after surgery (T1) and at least 12 months postoperatively (T2).

Comparison of classic and novel human astrovirus MLB and VA seroprevalence in HIV and non-HIV cohorts in South China demonstrates high seroreactivity to classic human astrovirus, which is associated with HIV infection.

Unlabelled: Human astroviruses (HAstVs), including classic and novel clades (MLB, VA), are enteric pathogens with increasing clinical significance, particularly in immunocompromised populations. This cross-sectional study investigated HAstV seroprevalence and antibody response profiles in 197 individuals (101 HIV-positive and 96 HIV-negative) from Guangdong Province, South China. Recombinant HAstV1, MLB2, and VA1 spike proteins were expressed in , structurally validated using AlphaFold2, and used in enzyme-linked immunosorbent assays-based serology with minimal cross-reactivity confirmed in murine models.

Three-Dimensional Changes of Masseter Muscle in Patients With Class III Asymmetry After Bimaxillary Orthognathic Surgery.

The present study was aimed to comprehensively evaluate changes in the masseter muscle in patients with Class III facial asymmetry after bimaxillary orthognathic surgery and explore potential correlations between masseter muscle changes and facial morphology or postoperative mandibular stability. Fifty-two eligible patients with Class III facial asymmetry who underwent Le Fort I osteotomy and bilateral sagittal split osteotomy (BSSO) with or without genioplasty between January 2019 and June 2023 were included. Cone-beam computed tomography (CBCT) was collected for each patient at 3 stages: 1 week preoperatively (T0), immediately after surgery (T1), and at least 12 months postoperatively (T2).

Enhancer-driven Shh signaling promotes glia-to-mesenchyme transition during bone repair.

Plp1-lineage Schwann cells (SCs) of peripheral nerve play a critical role in vascular remodeling and osteogenic differentiation during the early stage of bone healing, and the abnormal plasticity of SCs would jeopardize the bone regeneration. However, how Plp1-lineage cells respond to injury and initiate the vascularized osteogenesis remains incompletely understood. Here, by employing single-cell transcriptional profiling combined with lineage-specific tracing models, we uncover that Plp1-lineage cells undergoing injury-induced glia-to-MSCs transition contributed to osteogenesis and revascularization in the initial stage of bone injury.

Targeting endoplasmic reticulum stress-induced lymphatic dysfunction for mitigating bisphosphonate-related osteonecrosis.

Background: Bisphosphonates (BPs) are the first-line treatment to stop bone resorption in diseases, including osteoporosis, Paget's disease, multiple myeloma and bone metastases of cancer. However, BPs-related osteonecrosis of the jaw (BRONJ), characterized by local inflammation and jawbone necrosis, is a severe intractable complication. The cumulative inflammatory burden often accompanies impaired lymphatic drainage, but its specific impact on BRONJ and the underlying mechanisms remain unclear.

Microstructure and Mechanical Properties of NiTi Alloy Prepared by Double-Wire + Arc Additive Manufacturing Plus Heat Treatment.

In this study, NiTi shape memory alloy was prepared by double-wire + arc additive manufacturing plus heat treatment using TA1 and ER-Ni welding wires as the raw materials. The results show that the microstructural evolution from the bottom to top is NiTi + NiTi → NiTi + NiTi + NiTi → NiTi + NiTi + NiTi + NiTi + α-Ti. Complex thermal cycles led to the precipitation of NiTi, which improves the hardness of the matrix (B2), and the average hardness value of the top region reaches 550.

Matrix Stiffness of GelMA Hydrogels Regulates Lymphatic Endothelial Cells toward Enhanced Lymphangiogenesis.

Lymphatic vessel regeneration is crucial for various tissue engineering strategies, particularly in resolving inflammation and restoring tissue homeostasis. In our study, we focused on investigating how hydrogel matrix stiffness influences lymphatic endothelial cells (LECs) in promoting lymphatic vessel regeneration. Gelatin methacrylate (GelMA) was chosen as our biomaterial due to its versatility in tissue engineering and biofabrication.

Super-Enhancer Driven LIF/LIFR-STAT3-SOX2 Regulatory Feedback Loop Promotes Cancer Stemness in Head and Neck Squamous Cell Carcinoma.

Super-enhancers (SEs) have been recognized as key epigenetic regulators underlying cancer stemness and malignant traits by aberrant transcriptional control and promising therapeutic targets against human cancers. However, the SE landscape and their roles during head and neck squamous cell carcinoma (HNSCC) development especially in cancer stem cells (CSCs) maintenance remain underexplored yet. Here, we identify leukemia inhibitory factor (LIF)-SE as a representative oncogenic SE to activate LIF transcription in HNSCC.

Fluorescence and lasing of neutral nitrogen molecules inside femtosecond laser filaments in air: mechanism and applications.

High power femtosecond laser pulses launched in air undergo nonlinear filamentary propagation, featuring a bright and thin plasma channel in air with its length much longer than the Rayleigh length of the laser beam. During this nonlinear propagation process, the laser pulses experience rich and complex spatial and temporal transformations. With its applications ranging from supercontinuum generation, laser pulse compression, remote sensing to triggering of lightning, the underlying physical mechanism of filamentation has been intensively studied.

Impaired autophagy-mediated macrophage polarization contributes to age-related hyposalivation.

Age-related dysfunction of salivary glands (SGs) leading to xerostomia or dry mouth is typically associated with increased dental caries and difficulties in mastication, deglutition or speech. Inflammaging-induced hyposalivation plays a significant role in aged SGs; however, the mechanisms by which ageing shapes the inflammatory microenvironment of SGs remain unclear. Here, we show that reduced salivary secretion flow rate in aged human and mice SGs is associated with impaired autophagy and increased M1 polarization of macrophages.

Integrative Multiomics Analyses Identify Molecular Subtypes of Head and Neck Squamous Cell Carcinoma with Distinct Therapeutic Vulnerabilities.

Substantial heterogeneity in molecular features, patient prognoses, and therapeutic responses in head and neck squamous cell carcinomas (HNSCC) highlights the urgent need to develop molecular classifications that reliably and accurately reflect tumor behavior and inform personalized therapy. Here, we leveraged the similarity network fusion bioinformatics approach to jointly analyze multiomics datasets spanning copy number variations, somatic mutations, DNA methylation, and transcriptomic profiling and derived a prognostic classification system for HNSCC. The integrative model consistently identified three subgroups (IMC1-3) with specific genomic features, biological characteristics, and clinical outcomes across multiple independent cohorts.

Comprehensive 3-dimensional Positional and Morphological Analyses of Condyle and Glenoid Fossa in Patients with Skeletal Class II Malocclusion following Bimaxillary Orthognathic Surgery.

This study aimed to comprehensively and quantitatively characterize 3-dimensional (3D) positional and morphological changes of the condyle and glenoid fossa in patients with skeletal Class II malocclusion treated with bimaxillary orthognathic surgery. Twenty eligible patients treated at our institution from January 2016 to December 2021 with more than 12 months of postoperative follow-up were retrospectively enrolled. Radiographic data of cone-beam computed tomography (CBCT) for each patient were collected at 3 stages: 1 week preoperatively (T0), immediately after surgery (T1), and at least 12 months postoperatively (T2).

Controlling the Polarization of Nitrogen Ion Lasing.

Air lasing provides a promising technique to remotely produce coherent radiation in the atmosphere and has attracted continuous attention. However, the polarization properties of N lasing with seeding have not been understood since it was discovered 10 years ago, in which the polarization behaviors appear disordered and confusing. Here, we performed an experimental and theoretical investigation of the polarization properties of N lasing and successfully revealed its underlying physical mechanism.

Spatial pattern of isoniazid-resistant tuberculosis and its associated factors among a population with migrants in China: a retrospective population-based study.

Background: Isoniazid-resistant, rifampicin-susceptible tuberculosis (Hr-TB) globally exhibits a high prevalence and serves as a potential precursor to multidrug-resistant tuberculosis (MDR-TB). Recognizing the spatial distribution of Hr-TB and identifying associated factors can provide strategic entry points for interventions aimed at early detection of Hr-TB and prevention of its progression to MDR-TB. This study aims to analyze spatial patterns and identify socioeconomic, demographic, and healthcare factors associated with Hr-TB in Shanghai at the county level.

Correction: RNAi-Mediated Silencing of Atp6i and Atp6i Haploinsufficiency Prevents Both Bone Loss and Inflammation in a Mouse Model of Periodontal Disease.

[This corrects the article DOI: 10.1371/journal.pone.

Corrigendum: Assessing heterogeneity of patient and health system delay among TB in a population with internal migrants in China.

[This corrects the article DOI: 10.3389/fpubh.2024.

A DEA game cross-efficiency based improved method for measuring urban carbon emission efficiency in China.

An accurate evaluation of carbon emission efficiency (CEE) at the city level can provide guidelines for understanding low carbon performance, which is crucial to achieving dual carbon targets. Existing CEE studies focused on national, industrial, and provincial scales while neglecting the city level and failing to consider competing relationships among decision-making units in their measurement models. To fill these gaps, this paper introduces the data envelopment analysis game cross-efficiency model (DEA-GCE) to measure urban CEE performance and compares it with the traditional Super-SBM model using the data from 283 Chinese cities between 2006 and 2019.

Assessment and evaluation of online education and virtual simulation technology in dental education: a cross-sectional survey.

Background: The global outbreak of coronavirus disease (COVID-19) has led medical universities in China to conduct online teaching. This study aimed to assess the effectiveness of a blended learning approach that combines online teaching and virtual reality technology in dental education and to evaluate the acceptance of the blended learning approach among dental teachers and students.

Methods: The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) checklist was followed in this study.

Assessing heterogeneity of patient and health system delay among TB in a population with internal migrants in China.

Backgrounds: The diagnostic delay of tuberculosis (TB) contributes to further transmission and impedes the implementation of the End TB Strategy. Therefore, we aimed to describe the characteristics of patient delay, health system delay, and total delay among TB patients in Shanghai, identify areas at high risk for delay, and explore the potential factors of long delay at individual and spatial levels.

Method: The study included TB patients among migrants and residents in Shanghai between January 2010 and December 2018.