Establishing an Artificial Old Yellow Enzyme (OYE)-Mediated Pathway in for Efficient Synthesis of 3-Phenyl-1-propanol.

The compound 3-phenyl-1-propanol is an important fragrance ingredient widely used in various industries, including food, beverages, cosmetics, and pharmaceuticals. However, its production mainly relies on chemical synthesis, which poses environmental concerns. Herein, we developed a sustainable and efficient method for 3-phenyl-1-propanol synthesis by constructing an artificial old yellow enzyme (OYE)-mediated pathway in BL21 (DE3).

Ovarian cancer targeted therapy: current landscape and future challenges.

Ovarian cancer is one of the deadliest malignancies in women and remains the leading cause of death from gynecological cancers worldwide. The standard treatment typically involves tumor-reducing surgery and cytotoxic chemotherapy; however, many patients are unable to tolerate the side effects of these treatments or experience recurrence due to significant drug resistance, which limits the overall clinical benefits. Consequently, there is a pressing need for novel therapeutic strategies.

Biocascade-inspired amplified oxygen vacancy effect on facet-engineered BiOI for ultrasensitive photoelectrochemical detection of 8-oxoguanine DNA glycosylase.

Sensitive detection of 8-oxoguanine DNA glycosylase 1 (hOGG1) activity is essential for early cancer screening and therapy, yet the potential of photoelectrochemistry (PEC) for hOGG1 detection is untapped. Herein, we explore a new bioreaction for sensitive PEC detection of hOGG1 through biocascade reaction provoked amplified oxygen vacancy (O) effect on facet-engineered BiOI. Specifically, the recognition of hOGG1 activated the catalytic peptide hydrolysis reaction of thrombin (Thr), producing the O stimulator p-aminophenol (AP).

Lysine enhances the photoresponsive oxidase-like activity of twin CdZnS for direct colorimetric detection of lysine.

Background: Lysine (Lys) is one of the eight essential amino acids for the human body, which can't be synthesized by the body and must be obtained from external sources. And the detection of Lys is of significance for disease monitoring. The construction of photoresponsive nanozymes based analytical methods have received increasing attention and have been successfully achieved for the detection of metal ions, small molecules and natural enzymes.

Colorimetric detection of methotrexate leveraging the halogen peroxidase-mimicking activity of BiWO nanoflowers.

This study explores BiWO nanoflowers as novel haloperoxidase (HPO) mimetics and their application in analytical science, aiming to develop an efficient colorimetric method for methotrexate (MTX) detection. BiWO nanoflowers were synthesized a modified hydrothermal method and exhibited bromoperoxidase- and iodoperoxidase-like activities, catalyzing the bromination of phenol red (PR) and iodination of thymol blue (TB). After optimizing the reaction conditions, the kinetic parameters, including the Michaelis-Menten constant () and maximum reaction velocity (), exceeded those of most of the reported HPO nanozymes.

A machine learning framework for predicting shear strength properties of rock materials.

The shear strength characteristics of rock materials, specifically internal friction angle and cohesion, are critical parameters for the design of rock structures. Accurate strength prediction can significantly reduce design time and costs while minimizing material waste associated with extensive physical testing. This paper utilizes experimental data from rock samples in the Himalayas to develop a novel machine learning model that combines the improved sparrow search algorithm (ISSA) with Extreme Gradient Boosting (XGBoost), referred to as the ISSA-XGBoost model, for predicting the shear strength characteristics of rock materials.

Off-label use of anlotinib in malignancies' treatment: efficacy and management of adverse reactions.

Anlotinib is a novel small-molecule multi-target tyrosine kinase inhibitor (TKIs) independently developed in China, it possesses the functions of inhibiting tumor angiogenesis and suppressing tumor growth. Anlotinib has achieved notable therapeutic effects in approved indications for advanced non-small cell lung cancer, soft tissue sarcoma, small cell lung cancer, and medullary thyroid carcinoma. Additionally, with unanimous expert consensus, it has been used off-label in various other tumors, yielding favorable outcomes.

Molecular and Biochemical Mechanisms of Scutellum Color Variation in Adults (Diptera: Tephritidae).

(Hendel) is an invasive fruit and vegetable pest, infesting citrus, mango, carambola, etc. We observed that the posterior thoracic scutella of some adults are yellow, some light yellow, and some white in China. Compared with the races with a yellow scutellum (YS) and white scutellum (WS), the race with a light-yellow scutellum (LYS) is dominant in citrus and carambola orchards.

Camellia saponin modulates oleic acid/linoleic acid-induced lipogenesis in human sebocytes through lipophagy activation.

Background: Oily skin not only threatens people with aesthetic and hygienic discomfort but also confronts them with annoying skin problems. To explore new skin care ingredients from herbal or plant extracts and understand their underlying mechanism for sebum control would assist in the discovery of desirable sebosuppressive agents, though it is still a deserving and challenging task.

Aim: To explore the effect of Camellia saponin (CS) on modulating the lipogenesis of human sebocytes.

Tea (Camellia sinensis) Seed Saponins Act as Sebosuppression Agents via the AMPK/mTOR Pathway.

Background: Excessive lipogenesis of the skin triggers some dermatological concerns, such as enlarged pores, acne, and blackheads. Although topical drug treatments can offer temporary relief, their prolonged usage may lead to side effects of dryness, irritation, or allergic reactions. Consequently, the development of safer and efficacious ingredients in cosmetics for managing sebum overproduction represents a significant yet challenging endeavor.

In situ surface oxygen vacancy effect synergistic with internal polarization effect in BiFeO for photoelectrochemical detection of T4 DNA ligase.

Recently, the field of cathode photoelectrochemistry has advanced significantly, yet there remained a dearth of innovative approaches in signal transmission strategies. This paper introduced a novel concept where the dopamine (DA)-engineered surface vacancy (Ov) effect on BiFeO microspheres synergistically interacted with the intrinsic polarization of the material, leading to a significantly enhanced photocurrent when compared to that of BiO or FeO alone without a built-in electric field. Based on this finding, we proposed a PEC biosensor that leveraged the competitive binding reaction between single-base nucleotides and DA for photocurrent output, wherein the T4 DNA ligase-mediated ligation reaction governed the production of single-base nucleotides.

Intranasal trivalent candidate vaccine induces strong mucosal and systemic immune responses against .

The spread of multidrug-resistant strains of poses a great challenge in gonorrhea treatment. At present, vaccination is the best strategy for gonorrhea control. However, given the extensive antigenic variability of , the effectiveness of monovalent vaccines is limited.

Application of computed tomography-based radiomics analysis combined with lung cancer serum tumor markers in the identification of lung squamous cell carcinoma and lung adenocarcinoma.

Objective: To establish a prediction model of lung cancer classification by computed tomography (CT) radiomics with the serum tumor markers (STM) of lung cancer.

Materials And Methods: Two-hundred NSCLC patients were enrolled in our study. Clinical data including age, sex, and STM (squamous cell carcinoma [SCC], neuron-specific enolase [NSE], carcinoembryonic antigen [CEA], pro-gastrin-releasing peptide [PRO-GRP], and cytokeratin 19 fragment [cYFRA21-1]) were collected.

Third-generation D-lactic acid production using red macroalgae Gelidium amansii by co-fermentation of galactose, glucose and xylose.

Macroalgae biomass has been considered as a promising renewable feedstock for lactic acid production owing to its lignin-free, high carbohydrate content and high productivity. Herein, the D-lactic acid production from red macroalgae Gelidium amansii by Pediococcus acidilactici was investigated. The fermentable sugars in G.

Target mediated bioreaction to engineer surface vacancy effect on BiOS nanosheets for photoelectrochemical detection of FEN1.

Photoelectrochemistry represents a promising technique for bioanalysis, though its application for the detection of Flap endonuclease 1 (FEN1) has not been tapped. Herein, this work reports the exploration of creating oxygen vacancies (Ov) in situ onto the surface of BiOS nanosheets via the attachment of dopamine (DA), which underlies a new anodic PEC sensing strategy for FEN1 detection in label-free, immobilization-free and high-throughput modes. In connection to the target-mediated rolling circle amplification (RCA) reaction for modulating the release of the DA aptamer to capture DA, the detection system showed good performance toward FEN1 analysis with a linear detection range of 0.

The Relationship between Quantitative Parameters of Dual-energy CT and HIF-1α Expression in Non-Small Cell Lung Cancer.

Objective: This study aimed to investigate whether there is a correlation between quantitative parameters of dual-energy computed tomography (DECT) and the relative expression of HIF-1α in patients with non-small cell lung cancer (NSCLC) to preliminarily explore the value of DECT in evaluating the hypoxia of tumor microenvironment and tumor biological behavior and provide more information for the treatment of NSCLC.

Methods: This retrospective research included 36 patients with pathologically confirmed NSCLC who underwent dual-energy enhanced CT scans. The quantitative parameters of DECT were analyzed, including iodine concentration, water concentration, the CT values corresponding to 40keV, 70keV, 100keV, and 130keV in arterial and venous phases, and the normalized iodine concentration and the slope of the energy spectrum curve were calculated.

Characterization of two novel hydrolases from Sphingopyxis sp. DBS4 for enantioselective degradation of chiral herbicide diclofop-methyl.

Diclofop-methyl, an aryloxyphenoxypropionate (AOPP) herbicide, is a chiral compound with two enantiomers. Microbial detoxification and degradation of various enantiomers is garnering immense research attention. However, enantioselective catabolism of diclofop-methyl has been rarely explored, especially at the molecular level.

Compound heterozygosity for Southeast Asian hereditary persistence of fetal hemoglobin and β0-thalassemia results in thalassemia intermedia: Pedigree analysis and genetic research in a family from South China. A case report.

Rationale: Compound heterozygotes for deletional β-thalassemia can be difficult to diagnose due to its diverse clinical presentations and no routine screenings. This can lead to disease progression and delay in treatment.

Patient Concerns: We reported pedigree analysis and genetic research in a family with rare β-thalassemia.

Characterization of protective immune responses against induced by intranasal immunization with adhesion and penetration protein.

Drug-resistant is an urgent threat to global public health, and vaccine development is the best long-term strategy for controlling gonorrhea. We have previously shown that adhesion and penetration protein (App) play a role in the adhesion, invasion, and reproductive tract colonization of . Here, we describe the immune response induced by intranasal immunization with passenger and translocator fragments of App.

Thioredoxin Reductase-Mediated Reaction Evokes Surface Polarization Effect on BiOIO: Toward a New Sensing Strategy for Cathodic Photoelectrochemistry.

We have witnessed the fast progress of cathodic photoelectrochemistry over the past decades, though its signal transduction tactic still lacks diversity. Exploring new sensing strategies for cathodic photoelectrochemistry is extremely demanding yet hugely challenging. This article puts forward a unique idea to incorporate an enzymatic reaction-invoked surface polarization effect (SPE) on the surface of BiOIO to implement an innovative cathodic photoelectrochemical (PEC) bioanalysis.

Cervicovaginal microbiota long-term dynamics and prediction of different outcomes in persistent human papillomavirus infection.

Persistent human papillomavirus (HPV) infection can lead to cervical intraepithelial neoplasia (CIN) and cervical cancer, posing serious threats to the health of women. Although the cervicovaginal microbiota is strongly associated with CIN, the dynamics of the microbiota during CIN development are unknown. In this retrospective cohort study, we analyzed 3-year longitudinal data from 72 patients diagnosed with a persistent HPV infection almost all caused by high-risk HPV types.

DegS protease regulates antioxidant capacity and adaptability to oxidative stress environment in .

Adaptation to oxidative stress is critical for survival of in aquatic ecosystems and hosts. DegS activates the σ envelope stress response. We have previously revealed that DegS may be involved in regulating the oxidative stress response.

The novel norcantharidin derivative DCZ5417 suppresses multiple myeloma progression by targeting the TRIP13-MAPK-YWHAE signaling pathway.

Background: Multiple myeloma (MM), an incurable disease owing to drug resistance, requires safe and effective therapies. Norcantharidin (NCTD), an active ingredient in traditional Chinese medicines, possesses activity against different cancers. However, its toxicity and narrow treatment window limit its clinical application.

Nitenpyram biodegradation by a novel nitenpyram-degrading bacterium, sp. strain DF-1, and its novel degradation pathway.

Nitenpyram is a neonicotinoid insecticide that is commonly found in the environment. However, its biodegradation by pure cultures of bacteria has not been widely investigated and the catabolic pathway (s) for nitenpyram metabolism remain elusive. In this study, the aerobic strain DF-1, isolated from a wastewater-treatment pool contaminated with nitenpyram.