Dynamic Proteomic and PTMomic Characterization of Mycobacteria after Clinical Pharmaceutical Intervention.

Tuberculosis, a major global health threat, necessitates understanding the pharmacological mechanisms of current drugs to combat multidrug resistance. In addition to alterations at the proteome level, the dynamic changes occurring at various levels of post-translational modifications (PTMs) following pharmacological intervention remain unclear. In our current study, we employed a quantitative proteomic approach to systematically analyze the dynamic molecular alterations at both the proteome and PTM levels in response to clinical drugs, including ethambutol, bedaquiline, moxifloxacin, and streptomycin.

Compact Ion-Pair Aggregates Dominated Electrolytes Enable High-Performance Low-Temperature Lithium-Ion Batteries.

Lithium-ion batteries (LIBs) are widely used due to their high energy density, long cycle life, and environmental friendliness. However, at sub-zero temperatures, the operation of LIBs is constrained by degraded electrolyte ion transport performance and severe charge transfer polarization. In this work, we proposed a strategy for the construction of solvation structures dominated by compact ion-pair aggregates (CIPAs) through weak lithium-solvent interactions design, which significantly improved the lithium-ion transference number and reduced the de-solvation energy barrier.

Evaluation of Patients with Liver Disease before Nonhepatic Surgery.

Patients with cirrhosis who require surgeries are at higher risk of surgical complications and mortality than patients without cirrhosis. Several tools are available to predict the risk of mortality after surgery in patients with cirrhosis and should be used as a standard part of the preoperative evaluation. Improving surgical outcomes requires multidisciplinary care to ensure proper patient selection, to determine the appropriate timing of surgery, and to optimize the medical management of cirrhosis.

Global prevalence and risk factors of varicose veins among health care workers: a systematic review and meta-analysis.

Aims: To explore the global prevalence of varicose veins among healthcare workers and their associated risk factors.

Background: Varicose veins are a common occupational disease among healthcare staff. To enhance the occupational health of this population, it is necessary to understand their prevalence and risk factors.

Blocking triggering receptors expressed on myeloid cell-1 alleviates alveolar epithelial cell senescence by inhibiting oxidative stress in pulmonary fibrosis.

Pulmonary fibrosis (PF) is an insidious, progressive, and fatal age-associated disease that occurs primarily in older adults and has a poor prognosis. Alveolar epithelial cell (AEC) senescence is the critical pathological mechanism of PF. The accumulation of oxygen radicals, commonly referred to as reactive oxygen species (ROS), strongly contributes to cellular senescence.

A machine learning-based nomogram for predicting graft survival in allograft kidney transplant recipients: a 20-year follow-up study.

Background: Kidney transplantation is the optimal form of renal replacement therapy, but the long-term survival rate of kidney graft has not improved significantly. Currently, no well-validated model exists for predicting long-term kidney graft survival over an extended observation period.

Methods: Recipients undergoing allograft kidney transplantation at the Organ Transplantation Center of the First Affiliated Hospital of Kunming Medical University from 1 August 2003 to 31 July 2023 were selected as study subjects.

Pharmacokinetics and Tissue Distribution Study for 2-(2-Fluorophenyl)-5-Phenyl-7-Alkoxy- [1,2,4]Triazole[1,5-a]Pyrimidine Antiepileptic Compounds in Rats.

The development of novel active molecules with a clear target has always been an urgent need for the treatment of epilepsy. Previously, we reported 2-(2-fluorophenyl)-5-phenyl-7-propyl-[1,2,4]triazolo[1,5-a]pyrimidine (10C) as a selective and positive modulator of GABA receptors, which exhibited excellent antiepileptic activity in mice with an ED value of 8.51 mg/kg.

Amyloid-β oligomers drive amyloid deposit and cascaded tau pathology of Alzheimer's disease in aged brains of non-human primates.

Alzheimer's disease (AD), the most prevalent form of dementia, disproportionately affects the elderly population. While aging is widely recognized as a major risk factor for AD, the precise mechanisms by which aging contributes to the pathogenesis of AD remain poorly understood. In our previous work, the neuropathological changes in the brains of aged cynomolgus monkeys (≥18 years old) following parenchymal cerebral injection of amyloid-β oligomers (AβOs) have been characterized.

Nodal Downstaging of Esophageal Cancer After Neoadjuvant Therapy: A Cohort Study and Meta-Analysis.

Background: In esophageal cancer, the ypN0 status after induction therapy could be categorized into two primary groups: "natural N0" (cN0/ypN0) and "down-staged N0" (cN+/ypN0). The assessment of cN status is typically based on clinical imagination or pathological regression. However, there is no standardized method for evaluating cN/ypN status.

Unveiling an innovative sustainable blue resource-ecofriendly extraction technique towards a circular economy: Optimization of natural deep eutectic solvent and ultrasonication synergistic pathways for type-I collagen refined recovery from discarded fish scales.

A vast sum of fish waste is being annually discarded by marine fishing industries imposing serious environmental pollution concerns. However, these aquatic discarded matters are captivating sources of collagen, a fibrous protein with eminent social and economic relevance. Collagen is conventionally recovered using outdated complex processes requiring many reagents, multiple steps, and extended periods.

High-throughput markerless pose estimation and home-cage activity analysis of tree shrew using deep learning.

Background: Quantifying the rich home-cage activities of tree shrews provides a reliable basis for understanding their daily routines and building disease models. However, due to the lack of effective behavioral methods, most efforts on tree shrew behavior are limited to simple measures, resulting in the loss of much behavioral information.

Methods: To address this issue, we present a deep learning (DL) approach to achieve markerless pose estimation and recognize multiple spontaneous behaviors of tree shrews, including drinking, eating, resting, and staying in the dark house, etc.

Novel hybrid system based on carboxymethyl chitosan hydrogel encapsulating drug loaded nanoparticles for prolonged release of Vancomycin in the treatment of bacterial infection.

Current bacterial infections clinical treatments, such as intravenous antibiotic administration and local injection, suffer from short action duration, repeated administrations, and severe cell toxicity. To address these limitations, it is imperative to develop sustained drug release system with prolonged antimicrobial effects. In this work, a hybrid system was prepared using EDC/NHS catalyzed crosslinking-based carboxymethyl chitosan (CMCS) hydrogel as a carrier to encapsulate biodegradable nanoparticles (NPs) loaded with vancomycin, an efficient antibacterial drug.

Metal-organic cages based catalytic hybrid hydrogels for enhanced wound healing: Antibacterial and regenerative effects of Zr-MOC/chitosan composites hydrogel.

Metal-organic cages (MOCs), assembled by the coordination of metal nodes with organic ligands, offer excellent solvent dispersion, functionalization potential, and abundant binding sites, making them ideal for hybrid hydrogel synthesis. Herrin, a novel Zr-MOC/CS hybrid hydrogel was developed by crosslinking Zr-based metal-organic cages (Zr-MOC) and chitosan (CS) using dibenzaldehyde-functionalized polyethylene glycol (DF-PEG) as crosslinker, marking the first instance of incorporating Zr-MOC into a hydrogel matrix. The composite hydrogel leverages the catalytic activity of Zr-MOC to convert trace HO into hydroxyl radicals (·OH), delivering enhanced antibacterial performance.

Efficacy of double filtration plasmapheresis combined with immunosuppressive agents in the treatment of severe lupus nephritis.

Objective: To evaluate the efficacy of double filtration plasmapheresis combined with immunosuppressive agents in the treatment of severe lupus nephritis.

Method: A retrospective analysis was conducted on the medical records of 102 cases of severe lupus nephritis treated between January 2021 and December 2022 in the General Practice Department at the Affiliated Hospital of North Sichuan Medical College. Patients who received immunosuppressive agents were included in the control group and those who received additional double filtration plasmapheresis were included in the observation group.

Activin A receptor type 1C single nucleotide polymorphisms associated with esophageal squamous cell carcinoma risk in Chinese population.

Background: Transforming growth factor-β (TGF-β) superfamily plays an important role in tumor progression and metastasis. Activin A receptor type 1C (ACVR1C) is a TGF-β type I receptor that is involved in tumorigenesis through binding to different ligands.

Aim: To evaluate the correlation between single nucleotide polymorphisms (SNPs) of ACVR1C and susceptibility to esophageal squamous cell carcinoma (ESCC) in Chinese Han population.

Automated high-resolution 3D inspection methods for sealant applications in aerospace based on line structured light.

Gluing is a critical step in aircraft sealing assembly, with glue profile inspection serving as the final quality assurance measure to ensure consistency and accuracy of the sealant coating, allowing timely detection and correction of defects to maintain assembly integrity and safety. Currently, existing glue inspection systems are limited to basic inspection capabilities, lack result digitization, and exhibit low efficiency. This paper proposes a 3D inspection technology for sealant coating quality based on line-structured light, enabling automated and high-precision inspection of sealant thickness, sealant width, positional accuracy, and overlap joint sealant contour through geometric computation.

Novel anoikis-related diagnostic biomarkers for aortic dissection based on machine learning.

Aortic dissection (AD) is one of the most dangerous diseases of the cardiovascular system, which is characterized by acute onset and poor prognosis, while the pathogenesis of AD is still unclear and may affect or even delay the diagnosis of AD. Anchorage-dependent cell death (Anoikis) is a special mode of cell death, which is programmed cell death caused by normal cells after detachment from extracellular matrix (ECM) and has been widely studied in the field of oncology in recent years. In this study, we applied bioinformatics analysis, according to the results of research analysis and Gene Ontology (GO), as well as Kyoto Encyclopedia of Genes and Genomes (KEGG), finally found 3 anoikis-related genes (ARGs) based on machine learning.

Improving the anti-autolytic ability of alkaline protease from Bacillus alcalophilus by a rationally combined strategy.

Detergent enzymes have been extensively developed as eco-friendly alternatives to harmful chemicals, with alkaline protease representing a significant portion of detergent enzyme sales. However, the self-cleavage function of alkaline protease impacts its activity and overall application. Therefore, a new rational combinatorial strategy is proposed based on self-molecular docking (Self-ZDOCK) and molecular dynamics (MD) simulations.

PRMT1-mediated methylation of ME2 promotes hepatocellular carcinoma growth by inhibiting ubiquitination.

The mitochondrial malic enzyme 2 (ME2), which is frequently elevated during carcinogenesis and may be a target for cancer therapy, catalyzes the conversion of malate to pyruvate. The processes controlling ME2 activity, however, remain largely unclear. In this work, we show that human hepatocellular carcinoma (HCC) tissues contain high levels of ME2 and that the methylation of ME2 stimulates the growth and migration of HCC cells.

Double-network polyphenol chitosan hydrogels with instant aldehyde-β-cyclodextrin-based structure as potential for treating bacterially infected wounds.

Treatment of multiple bacterial infected wounds by eliminating bacteria and promoting tissue regeneration remains a clinical challenge. Herein, dual-network hydrogels (CS-GA/A-β-CD) with snap-structure were designed to achieve curcumin immobilization, using gallic acid-grafted chitosan (CS-GA) and aldehyde-β-cyclodextrin (A-β-CD) crosslinked. A-β-CD were able to achieve rapid dissolution (≥222.

Survival benefit of living donor liver transplant for patients with hepatocellular carcinoma.

With the increasing incidence of hepatocellular carcinoma (HCC) in both the United States and globally, the role of liver transplantation in management continues to be an area of active conversation as it is often considered the gold standard in the treatment of HCC. The use of living donor liver transplantation (LDLT) and the indications in the setting of malignancy, both generally and in HCC specifically, are frequently debated. In terms of both overall survival and recurrence-free survival, LDLT is at least equivalent to DDLT, especially when performed for disease within Milan criteria.

Converting fruit peels into biodegradable, recyclable and antimicrobial eco-friendly bioplastics for perishable fruit preservation.

The development of biodegradable antimicrobial bioplastics for food packaging holds great promise for solving the pollution and safety problems caused by petrochemical plastics and spoiled food. Herein, a natural active-bioplastic synthesized from citrus peel biomass is presented for perishable fruit preservation. These plastics are characterized by the nanoscale entanglement and recombinant hydrogen bonding between the endogenous pectin, polyphenols and cellulose micro/nanofibrils.