A protocol for organoids from the gynecomastia patients.

Background: Gynecomastia, characterized by benign proliferation of male breast glandular tissue, is a prevalent condition with complex etiologies. However, the absence of effective models has hindered mechanistic investigations and therapeutic development.

Methods: In this study, we established and characterized organoids derived from the breast tissues of six male gynecomastia patients, including physiological, idiopathic, and hormone-related subtypes.

Effect of the new fixed-time artificial insemination technology based on novel hormone preparations in gilts.

This study evaluated the effect of a novel fixed-time artificial insemination (FTAI) protocol for gilts, which incorporates altrenogest tablets (ALT-T), long-acting recombinant porcine FSH (rpFSH), and recombinant hCG (rhCG). A total of 160 gilts were divided into the control (n = 80) and the new treatment (n = 80) groups. Control gilts received oral altrenogest solution (20 mg/day) for 18 days, followed by 1000 IU equine chorionic gonadotropin (eCG) administered 42 h post-treatment and 100 μg GnRH 80 h later.

Exploring Prospective Relationships of Emotion Regulation Difficulties With Eating Disorder Psychopathology and Eating-Related Psychosocial Impairment Among Older Adults in China.

Objective: Emotion regulation (ER) difficulties are a robust risk factor for eating disorder (ED) psychopathology. As symptoms are often overlooked or misattributed to ageing, these associations are unclear in older adults. This study investigated the longitudinal relationships between ER difficulties, ED psychopathology (e.

A Study of the Safety of Low Temperature Plasma Radiofrequency Ablation on Rat Sciatic Nerve.

Purpose: Low temperature plasma radiofrequency ablation (LTPRA) has been widely applied for widespread clinical use in a variety of disciplines. However, the safe distance to act in the vicinity of neural tissue has not been determined.

Methods: Adult male Sprague-Dawley rats were subjected to LTPRA surgery performed at 0mm, 1mm and 2mm from the sciatic nerve or by cutting only the skin muscle to expose the sciatic nerve.

Clonorchis sinensis-driven hepatocarcinogenesis via E2F1-CD24 transcriptional axis: mechanistic and therapeutic implications.

Background: Hepatocellular carcinoma (HCC) remains a global health burden, with disproportionately high mortality in China's Guangxi region, where endemic Clonorchis sinensis (C. sinensis) infection coincides with elevated HCC incidence. Preliminary single-cell sequencing revealed marked overexpression of cluster of differentiation 24 (CD24) in HCC tissues, suggesting its potential pathological role.

Interfacial Oxygen Locking via Gradient Structural Design: A Route to Air-Stable and High-Performance Sodium Layered Oxides.

In the pursuit of sustainable energy storage solutions, sodium-ion batteries have garnered significant attention due to the abundance and low cost of sodium precursors. However, critical issues such as moisture sensitivity, sluggish kinetics, and phase degradation, particularly the irreversible lattice oxygen redox and parasitic side reactions, have significantly constrained their development. Here, we create a coherent gradient lattice reconstruction on an O3-type layered oxide surface via nonaqueous solvent-assisted ion exchange to mitigate O loss and interfacial issues.

Aspirin Eugenol Ester Ameliorates HFD-Induced NAFLD in Mice via the Modulation of Bile Acid Metabolism.

Non-alcoholic fatty liver disease (NAFLD) is a highly prevalent condition worldwide and represents a major global health challenge. Pharmacological and pharmacodynamic results indicate that aspirin eugenol ester (AEE) performs various pharmacological activities. However, it is unclear whether AEE can ameliorate the NAFLD.

Tripartite influence and social comparison theories for explaining eating disorder psychopathology in Chinese boys and girls: A longitudinal network perspective.

The present study used longitudinal network analysis to investigate the applicability of the tripartite influence model (TIM) and social comparison theories in explaining eating disorder (ED) psychopathology among Chinese adolescents. A total of 1428 Chinese adolescents (607 boys, 821 girls) participated in a four-wave longitudinal study over 18 months. Separate within-person contemporaneous and temporal networks were estimated for boys and girls, both demonstrating adequate network stability.

promotes the progression of clear cell renal cell carcinoma via the TNF-α/NF-κB axis.

Background: Clear cell renal cell carcinoma (ccRCC) represents the predominant form of kidney cancer. The Ras homology (Rho) GTPases family plays essential roles in several tumors. However, few studies have reported the function and clinical value of the Rho GTPases family in ccRCC.

Clinical prediction model and 2-year mortality for multiple organ dysfunction in patients aged 80 years or older following hip fracture surgery: a prospective cohort study.

Background: The prediction of postoperative complications is vital in the management of hip fracture. This study specifically examined the occurrence of multiple organ dysfunction syndrome in the elderly (MODSE) following hip fracture surgery and explored its predictive model and correlation with long-term mortality.

Methods: This prospective cohort study included all patients aged 80 years and older who underwent hip fracture surgery at a tertiary orthopedic hospital between January 2020 and March 2021.

Integrated transcriptomics and metabolomics revealed improvement of the nervous system in old rats by aspirin eugenol ester.

Elderly groups usually have an inflammatory response in their bodies, and neuroinflammation is the main pathogenic mechanism of neurological diseases. Pharmacological and pharmacodynamic experiments showed that aspirin eugenol ester (AEE) had good anti-inflammatory, anti-oxidant and anti-hyperlipidemic effects. However, whether AEE can prevent neurological diseases in the old companion animal has not been explored.

Global, regional, and national burden of endocarditis, 1990-2021: a systematic analysis of the GBD 2021 study.

Background: Endocarditis is a serious cardiovascular infection associated with high mortality and disability. Its global epidemiological patterns and long-term trends have not been comprehensively characterized.

Methods: Data from the Global Burden of Disease (GBD) Study 1990-2021 were used to evaluate the prevalence, incidence, disability-adjusted life years (DALYs), and Deaths of endocarditis across 204 countries and territories.

Prevalence and predictive importance for short-term clinical outcomes of GLIM-defined malnutrition among adult inpatients: A nationwide cross-sectional study.

Background & Aims: Malnutrition is a significant health issue in hospital settings, with persistent diagnostic challenges. Despite the Global Leadership Initiative on Malnutrition (GLIM) criteria providing a standardised approach, nationwide validation and implementation remain limited. We aimed to estimate the national prevalence of GLIM-defined malnutrition among Chinese adult inpatients and assess the predictive value of the GLIM criteria for short-term clinical outcomes.

Analysis of red blood cell lifespan and associated influencing factors in anemic dialysis patients.

Introduction: Renal anemia is one of the most common complications in dialysis patients. The shortened red blood cell (RBC) lifespan is an important mechanism of renal anemia. This study aims to investigate the RBC lifespan and its influencing factors in anemic dialysis patients.

Comparative efficacy of fascia iliaca compartment block using liposomal bupivacaine versus ropivacaine for preoperative analgesia in elderly patients with intertrochanteric femur fracture: a randomised controlled trial protocol.

Introduction: Intertrochanteric femur fractures are common in the elderly, with effective pain management being crucial. Fascia iliaca compartment block (FICB) is a well-established method for preoperative analgesia in these patients, but whether liposomal bupivacaine is more effective for this application remains uncertain. This trial aims to evaluate whether liposomal bupivacaine provides superior preoperative analgesia compared to ropivacaine in elderly patients with intertrochanteric femur fracture.

Combination therapy for platinum-resistant ovarian cancer: a novel at-home regimen with envafolimab, lenvatinib, and etoposide.

Background: Effective and tolerable treatment for patients with platinum-resistant recurrent ovarian cancer remains a great challenge in clinical practice. This study aimed to evaluate the efficacy and safety of envafolimab, the first subcutaneously administered programmed death ligand 1 (PD-L1) inhibitor, combined with lenvatinib and etoposide in patients with platinum-resistant recurrent ovarian cancer.

Methods: This was an open-label, single-arm, phase 2 trial (ENLEN-OC-001).

Resistance of spp. Causing Strawberry Black Spot to Boscalid in China.

Strawberry black spot, caused by spp., is an emerging disease that threatens both leaves and fruits during strawberry growth and postharvest storage. This study investigated the boscalid sensitivity of 49 isolates collected from symptomatic strawberry leaves.

Analysis and review of endoscopic features of submucosal tunnel endoscopic resection in the treatment of intramural esophageal bronchogenic cysts.

Background And Aims: Intramural esophageal bronchogenic cyst (EBC) is a rare congenital malformation. Due to few reports, there are still shortcomings in the overview of endoscopic characteristics and the selection of treatment methods. This study aimed to evaluate the endoscopic characteristics of EBCs and the safety and reliability of submucosal tunnel endoscopic resection (STER) for EBCs through case collection and literature review.

[Comparative study on implantation safety and stability of S and S sacral alar-iliac screws for sacroiliac joint fixation].

Objective: To explore the differences in the implantation safety and stability of a S alar-iliac screw (S1AIS) or S2AIS for sacroiliac joint fixation, providing reference for selecting appropriate internal fixation in clinical practice.

Methods: Patients who underwent pelvic CT examination between January 2024 and December 2024 were selected. CT data from 80 patients with normal pelvic structure who met the selection criteria were included in a 1∶1 male to female ratio.

Effect of pilates on the physical and mental health of drug-dependent individuals - a randomized controlled trial.

Objective: Drug-dependent individuals often face severe physical and mental health impairments, necessitating safe and adaptable rehabilitation strategies. This study aims to investigate the effects of Pilates exercise on the physical and mental health of drug-dependent individuals. Specifically, the study seeks to evaluate changes in body composition, physical fitness, blood biochemistry, and psychological outcomes following a structured Pilates intervention.

A 3-Year Environmental Surveillance Reveals Multiple Enteroviruses Including Types A76, A89, A90 and C113 in Eastern China.

Since molecular typing method of enterovirus was introduced, many new types have been discovered. However, due to the low epidemic potential, the information on newer enteroviruses remains limited globally. This study aims to investigate the diversity and phylogeny of newer enterovirus types through environmental surveillance utilizing next-generation sequencing (NGS) technology.

B4M3 CAR-T cell enhance antitumor activity and non-tumor toxicity in ovarian cancer.

Chimeric antigen receptor (CAR) T cells have demonstrated promising therapeutic outcomes in hematologic malignancies, but efficacy against most solid tumors, including ovarian cancer (OC). To address CAR-T challenges, we generated CAR-T cells (B4M3 CAR-T) targeting two tumor-associated antigens, B7H3 and MSLN, simultaneously. Immunohistochemistry and proteomics technologies, were employed to analyze the xenograft tumor tissues and key organ tissues at the end of the treatment in vivo assays.

Identification of metabolic reprogramming-related key genes in hepatocellular carcinoma after transcatheter arterial chemoembolization treatment.

Background: Metabolic reprogramming plays an important role in therapeutic efficacy of hepatocellular carcinoma (HCC). However, the metabolic reprogramming-related key genes associated with transcatheter arterial chemoembolization (TACE) treatment sensitivity in HCC remain further investigation.

Methods: We analyzed data from public databases, The Cancer Genome Atlas and Gene Expression Omnibus, as well as metabolism-related genes (MRGs), to identify key genes associated with TACE treatment sensitivity.

Enhanced Language Models for Predicting and Understanding HIV Care Disengagement: A Case Study in Tanzania.

Summary: Sustained engagement in HIV care and adherence to antiretroviral therapy (ART) are essential for achieving the UNAIDS "95-95-95" targets. Despite increased ART access, disengagement from care remains a significant issue, particularly in sub-Saharan Africa. Traditional machine learning (ML) models have shown moderate success in predicting care disengagement, which would enable early intervention.