Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: Gynecomastia, characterized by benign proliferation of male breast glandular tissue, is a prevalent condition with complex etiologies. However, the absence of effective models has hindered mechanistic investigations and therapeutic development.
Methods: In this study, we established and characterized organoids derived from the breast tissues of six male gynecomastia patients, including physiological, idiopathic, and hormone-related subtypes. Organoid fidelity was evaluated using hematoxylin and eosin (H&E) staining, immunohistochemistry (IHC), immunofluorescence (IF), and quantitative PCR (qPCR), targeting a panel of lineage-specific and proliferative markers.
Results: The organoids recapitulated key histological and molecular features of their corresponding source tissues, including epithelial architecture and expression of CK14, CK18, Ki67, and ERα. Marker expression was generally consistent between organoids and tissues at both the protein and transcriptional levels. Notably, ERα protein levels were reduced in organoids, while ESR1 mRNA expression remained stable, suggesting post-transcriptional regulation related to culture conditions.
Conclusion: Our study presents a practical and reproducible protocol for generating gynecomastia-derived organoids and highlights their utility as a disease-relevant platform for future research in male breast pathology and hormone-related mechanisms.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12381642 | PMC |
| http://dx.doi.org/10.3389/fbioe.2025.1593368 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Benefit-risk of colchicine and spironolactone in acute myocardial infarction: a prespecified generalised pairwise comparisons analysis of the CLEAR trial.
Heart
September 2025
Population Health Research Institute, Hamilton, Ontario, Canada.
Background: Composite outcomes in cardiovascular trials often group events of unequal clinical importance, and conventional analyses may obscure treatment trade-offs. Generalised pairwise comparisons (GPC), expressed as a win ratio (WR), allow for hierarchical ranking of events and incorporation of recurrent outcomes, providing a potentially more intuitive assessment of benefit-risk.
Methods: In a prespecified exploratory analysis of the 2×2 factorial, randomised CLEAR (Colchicine and Spironolactone in Patients with Myocardial Infarction) trial (7062 patients within 72 hours of acute myocardial infarction (MI) and percutaneous coronary intervention), we applied both time-to-first and recurrent-event GPC to reassess low-dose colchicine (0.
[Multi-system involvement characteristics and influencing factors of disease progression in Kennedy's disease].
Zhonghua Nei Ke Za Zhi
September 2025
Department of Neurology of the First Medical Center, Chinese PLA General Hospital, Beijing 100853, China.
To investigate multi-system involvement in Kennedy's disease and its association with disease progression. We retrospectively reviewed the clinical, laboratory, and electrophysiological data from 48 genetically confirmed patients with Kennedy's disease at the Department of Neurology, First Medical Center of the Chinese PLA General Hospital, between February 2016 and February 2024. The disease progression rate was calculated based on the functional scores at baseline and follow-up.
View Article and Find Full Text PDFAnthropometric Reference Values for the Male Thorax in Mexican Adults: Implications for Chest Surgery.
Cureus
July 2025
Plastic and Reconstructive Surgery, Hospital Central Sur de Alta Especialidad de Pemex, Mexico City, MEX.
Introduction Anatomical information regarding the nipple-areola complex (NAC) in men is limited. Our objective was to perform thoracic anthropometry in Mexican men and determine whether age and body mass index (BMI) influence the position and size of the NAC. Materials and methods Sociodemographic data and direct thoracic measurements were collected from 100 male subjects between January and June 2024 by the same physician.
View Article and Find Full Text PDFUsing Ultrasound and Power-Assisted Devices through Lateral Incision: The OCCULT Technique for Gynecomastia-A Multicentric Large Series Study.
Indian J Plast Surg
August 2025
Department of Plastic and Reconstructive Surgery, Excel Hospital, C K Birla Hospital, Rosewalk Hospital, New Delhi, India.
Introduction: Gynecomastia is a common condition characterized by male breast enlargement, which can have a profound psychological impact on affected individuals. Surgical intervention is often sought to alleviate these concerns. This study evaluates the lateral port technique for gynecomastia surgery, which combines power-assisted liposuction (PAL) and ultrasound-assisted liposuction (UAL) to achieve optimal surgical outcomes with no scar on front of chest.
View Article and Find Full Text PDFGynecomastia Surgery: Liposuction Alone versus Liposuction with Endoscope-Assisted Glandular Excision-A Comparative Study.
Indian J Plast Surg
August 2025
Department of Plastic Surgery, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.
Background: Gynecomastia, affecting around 30% of young males, has seen evolving surgical treatments, transitioning from traditional excision methods to contemporary techniques like liposuction. Emotional distress persists when glandular tissue is inadequately addressed, prompting exploration of combined liposuction and glandular excision procedures.
Materials And Methods: Patients undergoing gynecomastia surgery over a period of 2 years were assessed, considering their demographics, medical history, and gynecomastia grade.