Contact Lens Induce Blepharoptosis Through Fibrosis of Müller's Muscle.

Objective: The aim of this study was to observe and analyze the histologic characteristics and changes of the upper eyelid regarding blepharoptosis induced by wearing contact lens.

Methods: The levator aponeurosis-Müller's muscle complex specimens from 12 people who wear contact lenses were stained with hematoxylin-eosinand, Masson trichrome and antismooth muscle actin antibodies, and then were observed under light microscopy. The upper eyelids from 6 cadaver specimens were used as controls, and were examined with the same histologic techniques.

Environmental cadmium exposure aggravates renal fibrosis and reduced renal lipid accumulation in diabetic mice.

Background: Cadmium (Cd) is a heavy metal with nephrotoxicity. While the harmful effects of Cd exposure on the kidney have been extensively studied, the nephrotoxicity of Cd exposure on diabetic individual remain largely unexplored. Given the widespread Cd pollution and the high prevalence of diabetes, it is imperative to explore the nephrotoxicity and underlying mechanism of Cd exposure on diabetic individual.

Anatomical and Histological Study of Orbital Fat and Subcutaneous Fat.

Objective: Through intraoperative observation, histological analysis of specimens, and animal experiments on fat transplantation, we initially explore the histological characteristics of fat from different parts and provide theoretical support for related clinical operations.

Methods: The discarded orbital fat and subcutaneous fat tissue were collected from 18 patients who underwent upper eyelid and abdominal surgery at the same time, and 9 of them were stained with HE, Masson, and immunofluorescence to observe the histological characteristics of orbital fat and abdominal fat. The other 9 cases were transplanted to both sides of the back of 6-week-old nude mice.

Dissecting the physics of bacterial biofilms with agent-based simulations.

Biofilms are surface-attached bacterial communities encased within extracellular matrices (ECMs) of biopolymers that play many significant roles in health and society. Biofilms are versatile, living biomaterials that are resilient to a wide range of external perturbations, primarily due to the ECM, which consists of a complex network of polymeric macromolecules. Newly established platforms for live biofilm imaging at single-cell resolution have revealed a wealth of novel insights into the emergence of cellular organization within a developing biofilm.

Single-cell imaging reveals spontaneous phenotypic sorting and bet-hedging in developing biofilms.

How phenotypic heterogeneity shapes biofilm architecture and development remains poorly understood. Motivated by this, we developed imaging tools to track intracellular levels of c-di-GMP, a key second messenger that controls the motile-to-sessile transition, in developing biofilms at single-cell resolution. We show that c-di-GMP levels spontaneously bifurcate into a bimodal distribution that forms a spatially sorted pattern: High-c-di-GMP cells dominate the biofilm core while low-c-di-GMP cells localize to the periphery.

Multidentate ether modifies solvation structure in nonflammable phosphate electrolytes for wide-temperature lithium-ion batteries.

Conventional carbonate electrolytes cannot meet the requirements for lithium-ion batteries (LIBs) with wide temperature range and high safety. Diethyl ethylphosphonate (DEEP) has excellent flame retardancy and a wide temperature range (-83 to 198 °C), which holds promise for developing a non-flammable electrolyte for wide-temperature application in LIBs. However, its limited compatibility with graphite electrodes and slow ionic transport capability must be addressed.

The Impact of Nitrogen and Phosphorus Interaction on Growth, Nutrient Absorption, and Signal Regulation in Woody Plants.

This article methodically reveals how, in woody plants (poplar), the interaction between N and P coordinates root structure and nutrient absorption through a complex hormone signaling network. This study bridges a significant gap in our knowledge of nutrient interaction networks. The results demonstrate that NO significantly enhances the gene expression and enzymatic activity of organic acid synthases (MDH, PEPC) and APs.

Electrostatic interaction of neutral particles on a dielectric substrate: A theoretical study via a multiple-image method and an effective-dipole approach.

A multiple-image method is developed to accurately calculate the electrostatic interaction between neutral dielectric particles and a uniformly charged dielectric substrate. The difference in dielectric constants between the particle and the solvent medium leads to a reversal of polarization direction of the particle. The variance in dielectric constants between the solvent medium and the substrate causes a transition from attractive to repulsive forces between the particle and the substrate.

Single-Cell Multiomic Analysis of Circadian Rhythmicity in Mouse Liver.

From bacteria to humans, most organisms showcase inherent 24-hour circadian rhythms, best exemplified by the sleep-wake cycle. These rhythms are remarkably widespread, governing hormonal, metabolic, physiological, and behavioral oscillations, and are driven by "molecular clocks" that orchestrate the rhythmic expression of thousands of genes throughout the body. Here, we generate single-cell RNA and ATAC multiomic data to simultaneously characterize gene expression and chromatin accessibility of ~33,000 mouse liver cells across the 24-hour day.

Recycling of Waste Cathode Materials for Lithium-Ion Batteries by Deep Eutectic Solvents.

The growing demand for recycling of spent lithium-ion batteries (LIBs) drives the need for sustainable methods to recover strategic metals from cathode materials. Deep eutectic solvents (DESs) emerge as green alternatives to conventional acid leaching, offering advantages in selective extraction, reduced chemical usage, and energy efficiency. This review first elucidates the formation mechanisms of DESs and investigates how the parameter optimization of their physicochemical properties (e.

SERS-Based Immunochromatographic Assay for Sensitive Detection of O157:H7 Using a Novel WS-Au Nanotag.

O157:H7 contamination in food and the environment poses a serious threat to human health. Rapid and sensitive identification of foodborne pathogens remains challenging. Here, we prepared tungsten disulfide (WS)-Au nanocomposites coupled with the Raman signal molecule 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB) and antibodies to replace the conventional colloidal gold nanoparticles and applied SERS-active nanotags in the SERS-ICA method for highly sensitive detection of O157:H7.

Preparation, characterization, and antibacterial properties of a soybean protein isolate/gelatin composite film containing rosemary-modified bentonite and application of fresh lemon slices.

To address the rapid deterioration of fruit slices, soy protein isolate (SPI) and gelatin (GEL) composite films were optimized for food packaging using rosemary-modified bentonite (RB) as an active ingredient and glycerol as a plasticizer to enhance flexibility. Composite films with RB demonstrated superior ultraviolet-visible (UV-Vis) light barrier properties, increased opacity, and enhanced mechanical performance, achieving a tensile strength of 2.27 MPa.

Recycling and regeneration of failed layered oxide cathode materials for lithium-ion batteries.

With broad usage of lithium-ion batteries (LIBs) in electronic devices and electric vehicles (EVs), a large number of decommissioned LIBs will be generated, which cause serious environmental pollution and waste of resources. Therefore, to reduce environmental pressure and realize secondary resource utilization of valuable metals, recycling decommissioned LIBs is urgent. At present, although pyrometallurgy, hydrometallurgy, direct regeneration and other methods have been used significantly in the recovery of failed LIB cathode materials, it is still necessary to formulate the best recovery strategy to achieve higher recovery efficiencies and value-added materials from spent cathodes.

Electroactive membranes enhance in-situ alveolar ridge preservation via spatiotemporal electrical modulation of cell motility.

Post-extraction alveolar bone resorption invariably compromises implant placement and aesthetic restoration outcomes. Current non-resorbable membranes exhibit limited efficacy in alveolar ridge preservation (ARP) due to insufficient cell recruitment and osteoinductive capabilities. Herein, we introduce a multifunctional electroactive membrane (PPy-BTO/P(VDF-TrFE), PB/PT) designed to spatiotemporally regulate cell migration and osteogenesis, harmonizing with the socket healing process.

SERRATE drives phase separation behaviours to regulate m6A modification and miRNA biogenesis.

The methyltransferase complex (MTC) deposits N6-adenosine (mA) onto RNA, whereas the microprocessor produces microRNA. Whether and how these two distinct complexes cross-regulate each other has been poorly studied. Here we report that the MTC subunit B tends to form insoluble condensates with poor activity, with its level monitored by the 20S proteasome.

On-Line Measurement of Tracking Poses of Heliostats in Concentrated Solar Power Plants.

The tracking pose of heliostats directly affects the stability and working efficiency of concentrated solar power (CSP) plants. Due to occlusion, over-exposure, and uneven illumination caused by mirror reflection, traditional image processing algorithms showed poor performances on the detection and segmentation of heliostats, which impede vision-based 3D measurement of tracking poses of heliostats. To tackle this issue, object detection using deep learning neural networks are exploited.

Arctigenin ameliorates high-fat diet-induced metabolic disorders by reshaping gut microbiota and modulating GPR/HDAC3 and TLR4/NF-κB pathways.

Background: Arctigenin (AG), a phenylpropanoid lignan from the medicinal and food homologous plant Arctium lappa l., is known for its anti-cancer, anti-inflammatory and antioxidant properties. However, the pharmacological effects of AG on metabolic disorders remain limited, and specific mechanisms based on gut microbiota have not been reported.

Blind Quantum Machine Learning with Quantum Bipartite Correlator.

Distributed quantum computing is a promising computational paradigm for performing computations that are beyond the reach of individual quantum devices. Privacy in distributed quantum computing is critical for maintaining confidentiality and protecting the data in the presence of untrusted computing nodes. In this Letter, we introduce novel blind quantum machine learning protocols based on the quantum bipartite correlator algorithm.

Evaluation of cadmium effects on the glucose metabolism on insulin resistance HepG2 cells.

Cadmium (Cd) is an environmental endocrine disruptor. Despite increasing research about the metabolic effects of Cd on HepG2 cells, information about the metabolic effects of Cd on insulin resistance HepG2 (IR-HepG2) cells is limited. Currently, most individuals with diabetes are exposed to Cd due to pollution.

RNA helicase Brr2a promotes miRNA biogenesis by properly remodelling secondary structure of pri-miRNAs.

RNA secondary structure (RSS) of primary microRNAs (pri-miRNAs) is a key determinant for miRNA production. Here we report that RNA helicase (RH) Brr2a, best known as a spliceosome component, modulates the structural complexity of pri-miRNAs to fine tune miRNA yield. Brr2a interacts with microprocessor component HYL1 and its loss reduces the levels of miRNAs derived from both intron-containing and intron-lacking pri-miRNAs.

Reciprocal regulation of m A modification and miRNA production machineries via phase separation-dependent and -independent mechanisms.

Methyltransferase complex (MTC) deposits 6-adenosine (m A) onto RNA, whereas microprocessor produces miRNA. Whether and how these two distinct complexes cross-regulate each other has been poorly studied. Here we report that the MTC subunit B (MTB) tends to form insoluble condensates with poor activity, with its level monitored by 20S proteasome.

Research progress of orbital fat in histology and cytology: A review.

Orbital fat is an adipose tissue located behind orbital septum and originates from mesoderm and neural crest in ectoderm. It has been found that the histologic structure of orbital fat is different from subcutaneous and visceral fat. In addition, the regeneration and anti-inflammatory ability of stem cells derived from orbital fat have attracted much attention in recent years.

Biofilms as self-shaping growing nematics.

Active nematics are the nonequilibrium analogue of passive liquid crystals. They consist of anisotropic units that consume free energy to drive emergent behaviour. Like liquid crystal molecules in displays, ordering and dynamics in active nematics are sensitive to boundary conditions.

Parallel degradome-seq and DMS-MaPseq substantially revise the miRNA biogenesis atlas in Arabidopsis.

MicroRNAs (miRNAs) are produced from highly structured primary transcripts (pri-miRNAs) and regulate numerous biological processes in eukaryotes. Due to the extreme heterogeneity of these structures, the initial processing sites of plant pri-miRNAs and the structural rules that determine their processing have been predicted for many miRNAs but remain elusive for others. Here we used semi-active DCL1 mutants and advanced degradome-sequencing strategies to accurately identify the initial processing sites for 147 of 326 previously annotated Arabidopsis miRNAs and to illustrate their associated pri-miRNA cleavage patterns.

Biosafety assessments of hexafluoropropylene trimer derivative as a fluorinated cooling fluid for electronics.

Unlabelled: The Internet Data Center (IDC) is one of the most important infrastructures in the field of information technology. The cooling system for heat dissipation of IDC is indispensable due to it generates a large amount of heat during its calculation process, which may potentially harm its normal operation. Electronic fluorinated fluids have been widely used in cooling systems of IDC with stable physical and chemical properties.