Heat shock protein A1L restricts influenza A virus by ubiquitination of NA.

Neuraminidase (NA) protein of influenza A virus (IAV), a tetrameric envelope glycoprotein critical for viral release and spread, has well-characterized enzymatic functions but poorly resolved host-pathogen interactions. However, host factors targeted by NA or those regulating NA activity are largely uncharacterized. Here, we identified host heat shock protein A1L (HSPA1L) as a novel NA-binding partner that suppresses IAV replication.

Prevalence of Toxocara infection and associated risk factors: a cross-sectional study in Zhejiang, China.

Background: Human toxocariasis, caused by the zoonotic parasites Toxocara canis (dog roundworm) and T. cati (cat roundworm), affects approximately 19% of the global population, ranking it among the most prevalent neglected infection of poverty. However, public awareness about this zoonotic disease has not yet been achieved in China.

Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4.

Background: Porcine circoviruses 3 (PCV3) and 4 (PCV4) are emerging pathogens with global implications for swine industry, disturbing the diagnosis of PCVs associated diseases due to a range of similar clinical symptoms and increasingly coinfections. A rapid and accurate method for detection of PCV3 and PCV4 is critical for controlling the transmission of associated disease.

Methods: We developed a duplex real-time recombinase aided amplification (RAA) assay for detection of both PCV3 and PCV4 simultaneously.

Characterization of novel highly pathogenic avian influenza A(H5N6) clade 2.3.4.4b virus in wild birds, East China, 2024.

• We reported a novel H5N6 HPAIV belonging to clade 2.3.4.

[An self-ligation-based method for constructing infectious DNA clone of porcine circovirus type 2].

The aim of this study was to establish a rapid method for constructing infectious clones of porcine circovirus type 2 (PCV2). In this study, we constructed circular infectious clones of PCV2 by seamless cloning technology, using the clinically isolated strain PCV2-LX as a template. Meanwhile, this method was compared with the conventional restriction-ligation approach, focusing on the circularization (self-ligation) process of the genome and the growth characteristics of rescued viruses.