Stu2 performs an essential kinetochore function independent of its microtubule polymerase activity.

ch-TOG family proteins, including the budding yeast Stu2, are essential for spindle formation and chromosome segregation. Such functions depend on an array of activities ranging from microtubule nucleation, polymerization, and depolymerization to conferring tension sensitivity to kinetochores. This functional diversity makes it challenging to dissect these various functions and understand their relative importance.

Detection and characterization of a compound 1 species from the -type heme enzyme cytochrome '.

Compound 1 is a key intermediate in a large group of enzymes containing heme including cytochromes (cyt) P450, peroxidases and catalases. The extreme reactivity of this highly oxidized species has made its trapping and characterization challenging. Cyt ' of the ammonia-oxidizing bacterium is an enzyme containing heme and a member of the P460 superfamily of cytochromes.

Generating opposition to universal health care policies in the United States: An analysis of private health industry advertising on Meta platforms.

In 2019, the Partnership for America's Health Care Future (PAHCF), a private health industry lobby group, launched a campaign across Meta platforms (Facebook, Instagram) to generate opposition to universal health care policies in the United States. This study investigates the content and themes prevalent in PAHCF's campaign and how these might shape public discourse and perceptions around universal health care policies. Using qualitative content analysis, 1675 advertisements were examined on Meta platforms within PAHCF's campaign.

GOBoost: leveraging long-tail gene ontology terms for accurate protein function prediction.

Motivation: With the advancement of deep learning, researchers have increasingly proposed computational methods based on deep learning techniques to predict protein function. However, many of these methods treat protein function prediction as a multi-label classification problem, often overlooking the long-tail distribution of functional labels (i.e.

Beyond current boundaries: Integrating deep learning and AlphaFold for enhanced protein structure prediction from low-resolution cryo-EM maps.

Constructing atomic models from cryo-electron microscopy (cryo-EM) maps is a crucial yet intricate task in structural biology. While advancements in deep learning, such as convolutional neural networks (CNNs) and graph neural networks (GNNs), have spurred the development of sophisticated map-to-model tools like DeepTracer and ModelAngelo, their efficacy notably diminishes with low-resolution maps beyond 4 Å. To address this critical gap, this study introduces DeepTracer-LowResEnhance, an innovative computational framework that uniquely integrates structural predictions from AlphaFold with a deep-learning-based map refinement strategy specifically tailored to enhance low-resolution maps.

Digital activism in Kenya: moving from the digital center to the digital periphery of Long Covid experience.

Digital activism around Long Covid has reverberated around the globe, as patients, researchers, and clinicians worked together to understand the chronic condition. However, Long Covid networks, much like other social networks, have hierarchies and barriers that can impede equitable access. In this article, we examine how the global digital center and periphery shape how people with Long Covid connect to networks to learn about their illness symptoms, diagnoses, treatments, and experiences.

Why Public Health Nurses Matter: Bringing Specialized Knowledge and Skills to Advancing Health Equity.

Research is needed to better understand how public health nurses (PHNs) contribute to promoting health equity in communities. Our study aimed to fill this gap by exploring what activities PHNs describe they undertake in advancing health equity as well as examining their skills, proficiencies, and training needs specific to health equity work. We collected qualitative data via interviews with 18 PHNs across the US and developed major themes using a thematic analysis approach.

The impact of instruction on undergraduates' understanding of homeostasis: results from administering the homeostasis concept inventory.

The Homeostasis Concept Inventory (HCI) is a validated instrument for measuring students' knowledge of homeostasis. It is comprised of 20 multiple-choice questions covering key components of the previously validated Homeostasis Conceptual Framework (HCF). In this paper, we present the first multi-institutional study of the impact of physiology instruction on students' HCI performance.

Spillover Effects of an Agricultural Livelihood Intervention Among People Living with HIV on Community Empowerment in Kenya.

Food insecurity and HIV are closely intertwined and together have compounded effects on morbidity and mortality. Additionally, food insecurity and HIV have combined effects on weakening communities, including causing decreased economic capacity, decreased ability of families to care for children, and intergenerational transfer of poverty. Livelihood interventions that improve food security are promising approaches for improving the quality of life of people living with HIV, and they may also have profound effects on the strength and stability of communities.

Promoting Interest in Future Correctional Health Care Employment Through a Jail-Based Rotation for Resident Physicians.

Recruitment and retention of clinical staff in correctional settings remain a significant challenge. Few physicians learn about the provision of care within carceral institutions, most notably during their initial training programs when career trajectories are typically determined. A rotation for senior family medicine residents was developed in a county jail with an experiential learning curriculum that centers the needs of individuals experiencing incarceration.

Intercultural sensitivity among nursing educators: Comparison between Japan and China.

Background: Due to globalization, intercultural sensitivity is critical for nursing educators to train students for diverse cultural settings. However, research on factors influencing intercultural sensitivity among nursing educators is limited worldwide, including in Japan and China.

Aim: To compare the factors related to intercultural sensitivity among nursing educators in Japan and China.

Factors associated with cultural sensitivity and global nursing education among nurse educators: A cross-sectional study.

Background: Nurse educators must be culturally sensitive to teach cultural care to nursing students effectively.

Objective: To explore the factors associated with cultural sensitivity and global nursing education among nurse educators.

Methods: This was a cross-sectional exploratory study.

Exploring Sexual Harassment in Agriculture From a Socio-Ecological Perspective: A Scoping Review.

Workplace sexual harassment (WSH) and other forms of sexual violence are pervasive in the agricultural sector, yet remain overlooked as critical occupational health and safety concerns. In this scoping review, the social-ecological model was used as a framework to examine contributing and protective factors in the literature that inform WSH interventions, policy, and research. Using Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocols, the authors searched eight databases using Boolean terms related to "sexual harassment" and "agriculture.

Evidence for microbially-mediated tradeoffs between growth and defense throughout coral evolution.

Background: Evolutionary tradeoffs between life-history strategies are important in animal evolution. Because microbes can influence multiple aspects of host physiology, including growth rate and susceptibility to disease or stress, changes in animal-microbial symbioses have the potential to mediate life-history tradeoffs. Scleractinian corals provide a biodiverse, data-rich, and ecologically-relevant host system to explore this idea.

Analyzing the relationships among psychosocial variables associated with resilience.

Potentially Traumatic Events (PTEs) are common in current society, including college life. When exposed to PTE, stress reactions are greatly heterogeneous, and what contributes to psychological resilience is not well known. The purpose of the present study is to investigate the relationships among the antecedents, defining attributes, and consequences of resilience in a sample of 450 college students.

The ethics of knowledge sharing: a feminist examination of intellectual property rights and open-source materials in gender transformative methodologies.

Debates on intellectual property rights and open source frequently stem from the business sector and higher education, where goals are typically oriented toward profit, academic status, credit, and/or reputation. What happens if we reconsider the ethics of intellectual property rights and open source when our driving motivation is advancing women's health and rights? How does this prioritization complicate our assumptions of copyright and open access? How can we embark on a journey that validates the complex realities of multiple stakeholders who have good intent, but do not always consider the unintended impacts and the broader power dynamics at play? This paper explores the tensions and nuances of sharing methodologies that aim to transform harmful gender norms in an ecosystem that does not always consider the complex challenges behind intellectual property and open-source material. As a thought-collective dedicated to using a feminist approach to unpack and promote the principles of ethical, effective, and sustainable scale, we hope to underscore how the current research and debates on intellectual property rights and open-source material have good aims but may also fall short in encompassing the realities of gendered social norms change in and with communities around the world.

Constructing Sacred Containers for Participatory Action Research: An Embodied Mindfulness Arts-Based Practice.

College campuses are sites of institutional betrayal and interpersonal harm for too many survivors of gender-based violence. In pursuit of change aligned with empowerment frameworks and feminist epistemologies, many of us create spaces for impacted students to engage in participatory action research and learning. This article describes a mindfulness, arts-based embodied practice for transforming settings and positioning participants as visionaries and experts on safety, power, and well-being.

A rapid assessment of depressive and anxious symptoms among university students during the COVID-19 public health emergency: A repeated cross-sectional analysis.

Objectives: To identify potential associations between student characteristics and mental health symptoms during the early parts of the pandemic.

Participants: 3,883 students at a large public university on the West Coast of the United States.

Methods: We conducted a repeated cross-sectional survey to assess health-protective behaviors, mental health, social support, and stigma resistance.

Perceptions of the effects of recorded hypnosis and relaxation interventions for cancer survivors with chronic pain.

Background: Cancer survivors with chronic pain experience pain relief with hypnosis and relaxation approaches; however, the effects of hypnosis and relaxation audio recording interventions on chronic pain have not yet been described from the perspective of the cancer survivor. The purpose of this study was to better understand cancer survivors' experiences using hypnosis and relaxation interventions.

Materials And Methods: A randomized controlled trial with 109 cancer survivors experiencing chronic pain were assigned to the hypnosis (n = 55) or relaxation (n = 54) audio recordings.

Coral Disease: Direct and Indirect Agents, Mechanisms of Disease, and Innovations for Increasing Resistance and Resilience.

As climate change drives health declines of tropical reef species, diseases are further eroding ecosystem function and habitat resilience. Coral disease impacts many areas around the world, removing some foundation species to recorded low levels and thwarting worldwide efforts to restore reefs. What we know about coral disease processes remains insufficient to overcome many current challenges in reef conservation, yet cumulative research and management practices are revealing new disease agents (including bacteria, viruses, and eukaryotes), genetic host disease resistance factors, and innovative methods to prevent and mitigate epizootic events (probiotics, antibiotics, and disease resistance breeding programs).