Detection and characterization of a compound 1 species from the -type heme enzyme cytochrome '.

Compound 1 is a key intermediate in a large group of enzymes containing heme including cytochromes (cyt) P450, peroxidases and catalases. The extreme reactivity of this highly oxidized species has made its trapping and characterization challenging. Cyt ' of the ammonia-oxidizing bacterium is an enzyme containing heme and a member of the P460 superfamily of cytochromes.