Interaction studies by NMR on the multivalent interaction between chondroitin sulfate E derivatives and the langerin receptor.

In this paper, we present the NMR analysis of multivalent compounds displaying chondroitin sulfate E (CS-E) disaccharide ligands and their interaction with langerin. The disaccharides correspond to the two alternative sequences of CS-E: GlcA-GalNAc and GalNAc-GlcA. Firstly, we studied the conformation of the two corresponding series of glycodendrimers free in solution and in the presence of langerin.

Electrosurgical Catheter Reshaping to Unify a Double Atrial SEptal Defect (ERASED Procedure) for Single-Device Percutaneous Closure.

We present the first case of a double atrial septal defect (ASD) successfully reshaped using transcatheter electrosurgery to unify both defects into a single orifice, enabling closure with a single device. This technique appears feasible and may simplify the management of complex double ASDs in some cases.

User journeys in cross-European secondary use of health data: insights ahead of the European Health Data Space.

The European Health Data Space (EHDS) regulation aims to facilitate cross-border sharing of health data across Europe. However, practical challenges related to data access, interoperability, quality, and interpretive competence remain, particularly when working with health systems across countries. This study aimed to evaluate and report the user journey of researchers accessing and utilizing health data across four European countries for secondary research purposes prior to implementation of EHDS.

Genomic data sharing in research across Europe: legal challenges and upcoming opportunities within the European Health Data Space.

The European Health Data Space (EHDS) will help researchers use health data across EU Member States (MS). Currently, cross-border research faces heterogeneous data access processes. Using a real-world use case, this paper analyses challenges and opportunities brought by the upcoming implementation of the EHDS, assessing the situation before and after the regulation comes into force.

Experimental Evidence That Blood Parasite Infection Affects Incubation Patterns in a Cavity-Nesting Songbird.

Avian chronic hemoparasite infections occur commonly in wild birds, causing adverse effects on host fitness and breeding success. However, the potential impact of such infections on the incubation behavior has been scarcely experimentally studied. We reduced the infection of hemoparasites in wild-breeding female pied flycatchers (Ficedula hypoleuca) through medication with primaquine to test the possible effects on incubation patterns compared with non-medicated control females.

Heat drastically alters floral color and pigment composition without affecting flower conspicuousness.

Premise: Floral pigments primarily serve to attract pollinators through color display and also contribute to protection against environmental stress. Although pigment composition can be plastically altered under stress, its impact on pollinator color perception remains poorly understood. Moricandia arvensis (Brassicaceae) exhibits seasonal floral dimorphism, with lilac spring flowers and white summer flowers.

Dietary Consumption of Type 2 Resistant Starch and d-Fagomine Delays Progression of Metabolic Disturbances in Male Rats on High-Fat Diet.

High-fat (HF) diets contribute to obesity, insulin resistance, fatty liver, gut microbiota dysbiosis, oxidative stress, and low-grade chronic inflammation. This study evaluated the preventive effects of dietary Type 2 resistant starch (RS2) from high-amylose maize and low-dose d-fagomine (FG) from buckwheat on these metabolic disturbances. Male Wistar-Kyoto rats (9-10 weeks old) were assigned to four diet groups for 10 weeks: standard (STD) diet, HF diet (45% kcal from fat), HF + RS diet (15% RS2), and HF + FG diet (0.

Real World Evidence of Active Surveillance for Prostate Cancer in Spain; Midterm Results.

Patients And Methods: In this multicenter longitudinal study, data from the Spanish Register in AS (AEU-PIEM/2014/0001) were reviewed. The study focused on a cohort of AS patients registered between 2014 and 2019, featuring open inclusion criteria and diverse follow-up strategies.

Results: A total of 3315 AS patients were recruited, with 2881 and 434 categorized into the low and intermediate risk groups based on NCCN grouping at inclusion.

No evidence of evolutionary impact from size-selective harvesting on epigenetic and reproductive molecular markers in zebrafish (Danio rerio) gonads.

This study investigates whether size-selective harvesting induced heritable changes reflected in epigenetic and reproductive molecular markers in zebrafish (Danio rerio), thereby indicating potential evolutionary responses. We used an experimental harvest model where zebrafish populations were subjected to five generations of size-selective harvesting, followed by eight generations without harvesting in a controlled environment to examine evolutionarily fixed outcomes in response to harvest selection. We assumed the evolutionary adaptations to size-selection to have left a molecular legacy related to sexual development, as previous studies have shown that evolution of reproductive timing is a common response to size-selection.

Recent Developments in Catalytic Asymmetric Aziridination.

Aziridines, structurally related to epoxides, are among the most challenging and fascinating heterocycles in organic chemistry due to their increasing applications in asymmetric synthesis, medicinal chemistry, and materials science. These three-membered nitrogen-containing rings serve as key intermediates in the synthesis of chiral amines, complex molecules, and pharmaceutically relevant compounds. This review provides an overview of recent progress in catalytic asymmetric aziridination, focusing on novel methodologies, an analysis of the scope and limitations of each approach, and mechanistic insights.

The Key Role of Public Forests in Restoring Connectivity: an Application to Castilla y León Region, Spain.

Forests have been increasingly affected by natural disturbances and human activities. These impacts have caused habitat fragmentation and a loss of ecological connectivity. This study examines potential restoration pathways that reconnect the five largest forest cores in the Castilla y León region of Spain.

Emerging strategies, applications and challenges of targeting NAD in the clinic.

Beyond their classical functions as redox cofactors, recent fundamental and clinical research has expanded our understanding of the diverse roles of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) in signaling pathways, epigenetic regulation and energy homeostasis. Moreover, NAD and NADP influence numerous diseases as well as the processes of aging, and are emerging as targets for clinical intervention. Here, we summarize safety, bioavailability and efficacy data from NAD-related clinical trials, focusing on aging and neurodegenerative diseases.

The Solve-RD Solvathons as a pan-European interdisciplinary collaboration to diagnose patients with rare disease.

Despite advances in genomic diagnostics, the majority of individuals with rare diseases remain without a confirmed genetic diagnosis. The rapid emergence of advanced omics technologies, such as long-read genome sequencing, optical genome mapping and multiomic profiling, has improved diagnostic yield but also substantially increased analytical and interpretational complexity. Addressing this complexity requires systematic multidisciplinary collaboration, as recently demonstrated by targeted diagnostic workshops.

Postpartum depression in Ecuadorian women: does the quality of health care during childbirth matter?

Poor quality obstetric care can harm women's mental health, especially after childbirth. This study examines how the perceived quality of health services during childbirth is related to postpartum depression in Ecuador. Using data from 16,451 women in the 2018 National Health and Nutrition Survey, we applied probit and latent class probit models.

Indications for haematopoietic cell transplantation and CAR-T for haematological diseases, solid tumours and immune disorders: 2025 EBMT practice recommendations.

For over two decades, the EBMT has updated recommendations on indications for haematopoietic cell transplantation (HCT) practice based on clinical and scientific developments in the field. This is the ninth special EBMT report on indications for HCT for haematological diseases, solid tumours and immune disorders. Our aim is to provide guidance on HCT indications according to prevailing clinical practice in EBMT countries and centres.

Serum progesterone variability on embryo transfer day in hormone replacement therapy cycles using intramuscular injections during frozen embryo transfers.

Purpose: To assess the intra-individual variability of serum progesterone (P) levels on embryo transfer (ET) day, when the same dose of intramuscular progesterone (IM-P) was used in two consecutive hormone replacement therapy (HRT) frozen embryo transfer (FET) cycles.

Methods: A total of 75 patients undergoing two consecutive HRT-FET cycles in one year performed at Bahceci Ankara IVF Center between November 2019 and February 2022 were retrospectively analyzed. Serum P levels were measured at the 117th-119th hours of support by a single laboratory.

Personalised genomic strategies improve diagnostic yield in inherited retinal dystrophies: a stepwise, patient-centred approach.

Background: Inherited retinal dystrophies (IRDs) are a genetically heterogeneous group of conditions, with approximately 40% of cases remaining unresolved after initial genetic testing. This study aimed to assess the impact of a personalised genomic approach integrating whole-exome sequencing (WES) reanalysis, whole-genome sequencing (WGS), customised gene panels and functional assays to improve diagnostic yield in unresolved cases.

Subjects/methods: We retrospectively reviewed a cohort of 597 individuals with IRDs, including 525 probands and 72 affected relatives.

Highly protected areas buffer against aridity thresholds in global drylands.

Drylands are highly vulnerable to global-scale aridity thresholds that cause drastic reductions in their productivity. While protected areas may help buffer against the impact of aridification, their effectiveness in mitigating the aridity thresholds across global drylands remains virtually unknown. Here we assembled a global dataset of drylands and found that highly protected areas, which include national parks and wilderness areas, can buffer the emergence of aridity thresholds in ecosystem productivity by up to 0.

Air-test for assessing gas exchange and the potential benefit of alveolar recruitment manoeuvre in anaesthetized dogs.

Objective: To determine the use of Air-Test in ventilated, anaesthetized dogs for evaluating oxygen uptake and to determine its potential utility in guiding the decision to perform an alveolar recruitment manoeuvre (ARM).

Study Design: Retrospective cohort study.

Animals: A total of 25 client-owned dogs undergoing general anaesthesia.