Identification and Functional Characterization of a Geraniol Synthase UrGES from .

, a medicinal plant extensively used in traditional Chinese medicine, is an important plant source of terpenoid indole alkaloids (TIAs), but the mechanism of TIA biosynthesis at molecular level remains unclear. Geraniol synthase (GES) serves as a crucial enzyme in catalyzing the formation of geraniol from geranyl pyrophosphate (GPP) in various plants, but the functional characterization of the gene in has not been investigated. In this study, a was identified and characterized through genome mining and bioinformatic analysis.

Comparative Transcriptome and Metabolome Analyses Provide New Insights into the Molecular Mechanisms Underlying Taproot Development and Bioactive Compound Biosynthesis in vahl.

Background: vahl is a famous Chinese medicinal plant. The root is the main organ accumulating bioactive compounds, and its development is directly related to the yield and quality of the harvested However, the molecular mechanisms underlying the bioactive compound biosynthesis occurring during the root development of are unknown.

Method: Transcriptome and widely targeted metabolome analyses were performed to investigate gene expression and metabolite variation during the development of taproots.

Physiological, biochemical, and transcriptomic analyses reveal potential candidate genes of Platostoma palustre in response to salt stress.

Background: Salt stress poses a genuine threat to plants, impeding their growth, development, and yields. Platostoma palustre (Blume) A.J.

Genotyping-by-sequencing of Illicium difengpi highlights its potential genetic diversity and conservation status.

Illicium difengpi is a critically endangered plant endemic to the karst mountains of Guangxi, China. Because of its medicinal and ecological values, many efforts were made on assessing the medicinal usage, natural history, ex-situ conservation, and stress tolerance of I. difengpi.

Integrated study on the genome, transcriptome, and metabolome of Gelsemium elegans and mining of related enzyme genes involved in koumine biosynthesis.

Gelsemium elegans a plant of the Loganiaceae family, is highly toxic and contains many alkaloids of potential pharmacological value. Koumine, is the most abundant component of G. elegans, and belongs to monoterpene indole alkaloids and is valued in medical research as it has important anti-inflammatory and anxiolytic properties.

Identification of key modules and genes in response to high-temperature stress in Platostoma palustre based on WGCNA.

Platostoma palustre (Blume) A. J. Paton is one of the important medicinal and edible plants in China, and it is widely cultivated in tropical and subtropical regions of southern China.

Comparative nutrient profiling of three Murraya species through combined metabolomic and transcriptomic analyses.

Murraya, a valuable plant resource, plays a critical role in medicine, industry, and landscaping. Despite its significance, research on Murraya, as well as its development and utilization, remains limited. Therefore, investigating the metabolites and metabolic pathways within its germplasm is of considerable importance.

Chromosome-level genome assembly of an important ethnic medicinal plant Callicarpa nudiflora.

Callicarpa nudiflora is one of high medicinal and economic value plants in China, which was recorded in Chinese pharmacopoeia (2020 edition) and widely used to treat tropical bacterial infections, acute infectious hepatitis, and internal and external bleeding. In this study, we assembled the C. nudiflora genome with a size of approximately 597.

Encapsulation of astilbin in zein nanoparticles with fructo-oligosaccharides and caseinate as costabilizers: Formation, stability, bioavailability, and antioxidant capacity.

Zein-based nanoparticles (NPs) have attracted considerable attention as potential delivery systems for bioactive compounds. However, their application has been limited by poor stability and redispersibility. In this study, we addressed these challenges by fabricating zein nanocarriers using branching structural fructo-oligosaccharides (P-FOS) and sodium caseinate (NaCas) as costabilizers.

Metabolic and transcriptional analysis of tuber expansion in Curcuma kwangsiensis.

The tubers of Curcuma kwangsiensis are regarded as an important medicinal material in China. In C. kwangsiensis cultivation, tuber expansion is key to yield and quality, but the regulatory mechanisms are not well understood.

Physiological and metabolic responses of to cadmium stress.

Unlabelled: is a significant medicinal plant indigenous to China and Vietnam. In China, is mainly grown naturally on limestone mountains or is cultivated artificially in arable land. Heavy metal contamination in agricultural soil, particularly cadmium (Cd), poses serious threats to soil health, as well as the growth and productivity of .

A high-quality chromosome-level genome assembly of the traditional Chinese medicinal herb Zanthoxylum nitidum.

Dried roots of Zanthoxylum nitidum (2n=2x=70, family Rutaceae) was referred as "Liang-Mian-Zhen" in Chinese, acting as a valuable species due to its notable pharmacological activities. Herein, we combined PacBio HiFi data together with Hi-C mapping technology to construct a chromosome-scale reference genome assembly for Z. nitidum.

Genome-wide identification and expression analysis of the WRKY gene family reveal essential roles in abiotic stress responses and polysaccharides and flavonoids biosynthesis in Platostoma palustre (Blume) A. J. Paton.

Background: Platostoma palustre (Blume) A. J. Paton is an economic crop with medicinal and edible properties.

Complete chloroplast genome and phylogenetic analysis of Willd. ex Roem. & Schult. 1819 Caprifoliaceae.

Willd. ex Roem. & Schult.

Physiological and gene expression response mechanisms of dehydration process in Cinnamomum cassia (L.) J. Presl seeds.

Background: A critical factor in the storability of recalcitrant seeds is their moisture content (MC), but its effect on the viability of Cinnamomum cassia (L.) J. Presl (C.

Widely targeted metabolomics reveals the phytoconstituent changes in leaves and stems at different growth stages.

is an important edible and medicinal plant. To gain a comprehensive and clear understanding of the variation patterns of metabolites in , we employed the UPLC-MS platform along with widely targeted metabolomics techniques to analyze the metabolites in the stems and leaves of at different stages. Our results revealed a total of 1228 detected metabolites, including 241 phenolic acids, 203 flavonoids, 152 lipids, 128 terpenes, 106 amino acids, 79 organic acids, 74 saccharides, 66 alkaloids, 44 lignans, etc.

Nitrogen Sources Reprogram Carbon and Nitrogen Metabolism to Promote Andrographolide Biosynthesis in (Burm.f.) Nees Seedlings.

Carbon (C) and nitrogen (N) metabolisms participate in N source-regulated secondary metabolism in medicinal plants, but the specific mechanisms involved remain to be investigated. By using nitrate (NN), ammonium (AN), urea (UN), and glycine (GN), respectively, as sole N sources, we found that N sources remarkably affected the contents of diterpenoid lactone components along with C and N metabolisms reprograming in , as compared to NN, the other three N sources raised the levels of 14-deoxyandrographolide, andrographolide, dehydroandrographolide (except UN), and neoandrographolide (except AN) with a prominent accumulation of farnesyl pyrophosphate (FPP). These N sources also raised the photosynthetic rate and the levels of fructose and/or sucrose but reduced the activities of phosphofructokinase (PFK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphopyruvate carboxylase (PEPC) and pyruvate dehydrogenase (PDH).

Shade responses and resistant mechanisms in .

The medicinal plant Dunn is easily exposed to shade stress during growth, but its shade responses and shade stress resistant mechanisms have not been clarified. In this study, shade treatments including four attenuated sunlight intensities (100%, 60%, 40%, and 10%) and three shade durations (30 d, 45 d, and 60 d) were applied to . The shade-induced morphological indicators, phytohormonal regulations, metabolic flavonoids contents, transcriptomic flavonoid pathway gene expressions, and stress physiological changes of were analyzed.

Effects of different colors of plastic-film mulching on soil temperature, yield, and metabolites in Platostoma palustre.

Platostoma palustre is an annual herb and an important medicinal and edible plant in southern China. Plastic-film mulching is an effective agronomic practice in the cultivation system of P. palustre, of which black-film mulching is the most common.

Biochar and organic fertilizer drive the bacterial community to improve the productivity and quality of in cadmium-contaminated soil.

Excessive Cd accumulation in soil reduces the production of numerous plants, such as Gagnep., which is an important and widely cultivated medicinal plant whose roots and rhizomes are used in traditional Chinese medicine. Applying a mixture of biochar and organic fertilizers improved the overall health of the Cd-contaminated soil and increased the yield and quality of .

Cellular evidence of communication strategies for small intestinal high endothelial cells: Ultrastructural insights of nano-scale exosomes and autophagy.

Although several immune related cells of small intestine play an essential role in the intestinal homeostasis. However, information related to ultrastructural evidence of Nano-scale exosomes-multivesicular bodies and autophagic pathway in the high endothelial cells (HECs) of the small intestine in laying birds is still ambiguous. In present study, the HECs secreted the early endosome (ee), late endosome (le) and multivesicular bodies (MVBs) in the lamina propria of layer small intestine was confirmed by transmission electron microscopy.

Transcriptomics and metabolomics association analysis revealed the responses of to cadmium.

an important medicinal herb, can absorb high amounts of cadmium (Cd) which can lead to excessive Cd contamination during the production of medicines and tea. Hence, it is crucial to investigate the response mechanism of under Cd stress to develop varieties with low Cd accumulation and high tolerance. Physiological response analysis, transcriptomics and metabolomics were performed on seedlings exposed to Cd stress.

Sophora tonkinensis: response and adaptation of physiological characteristics, functional traits, and secondary metabolites to drought stress.

The medicinal plant Sophora tonkinensis is a characteristic Chinese shrub of karst areas. The arid climate in karst areas produces high-quality S. tonkinensis; however, the mechanisms of drought tolerance are not clear, which restricts sustainable plantings of S.