Mechanisms of interaction between bovine serum albumin and novel schiff base compounds: A multi-spectroscopic, DFT analysis and in silico investigation.

The synthesis of two Schiff base (SB) compounds, 3-phenyl-4-(5-nitrofuran-1-yl)methyleneamino-4,5-dihydro-1H-1,2,4-triazol-5-one (3‒PNM) and 1-acetyl-3-phenyl-4-(5-nitrofuran-1-yl)methyleneamino-4,5-dihydro-1H-1,2,4-triazol-5-one (1-APNM) was successful. The structures of 3-PNM and 1-APNM were determined using ¹H/¹C-NMR and IR spectroscopy. Absorption and fluorescence spectroscopy were used to evaluate the compounds' interactions with BSA.

In Vivo Wound-Healing and Molecular Docking Studies Support the Traditional Use of Arisarum vulgare Aqueous Extract.

In Algerian traditional medicine, Arisarum vulgare O. Targ. Tozz.

Exploring the acute recovery effects of dry cupping therapy and electrical stimulation on anaerobic performance metrics in active individuals: A quasi-experimental crossover study.

Background: This quasi-experimental crossover study aimed to examine the acute effects of dry cupping therapy (DCT) and neuromuscular electrical stimulation (NMES) on fatigue and perceived exertion in anaerobic performance among physically active individuals.

Methods: Twelve male participants (mean age: 24.1 ± 2.

Hybrid Nanoflower-enhanced Enzyme-linked Immunosorbent Assay: A Novel Platform for 8-Hydroxy-2'-deoxyguanosine Biomarker Detection.

Among the various oxidative DNA lesions, 8-hydroxy-2'-deoxyguanosine (8-OHdG) is widely recognized as a critical biomarker for assessing DNA damage and disease risk. Traditional detection methods, such as high-performance liquid chromatography (HPLC), LC-tandem mass spectrometry, and immunohistochemical techniques, provide high accuracy but suffer from limitations like time-consuming procedures, high costs, and extensive sample preparation requirements. Enzyme-linked immunosorbent assay (ELISA), a widely used and cost-effective alternative, has been increasingly explored for 8-OHdG detection.

Selenium-coated chitosan nanoparticles (CTS-Se NPs) improve grapevine (Vitis vinifera cv. Sultana) performance grown under lead (Pb) toxicity.

Background: Selenium-coated chitosan nanoparticles (CTS-Se NPs) have been proposed as innovative engineered nanoparticles with the potential to alleviate various abiotic stresses, thereby enhancing plant growth and productivity. However, evidence of their efficacy remains limited. In this study, the effects of CTS-Se NPs (10 and 20 mg L⁻¹), along with CTS NPs (0.

LC-ESI-MS/MS Profiling and In Vitro Evaluation of Antioxidant, Enzymatic Inhibitory, and Cytotoxic Activities, Along With Complementary In Vitro and In Silico Assessment of the Antibacterial Potential of Centaurea papposa Leaf Extracts.

The main objective of this study was to characterize the phenolic composition of the hydromethanolic extract and its fractions from Centaurea papposa leaves, and to evaluate their antioxidant, enzymatic inhibitory, cytotoxic, and antibacterial activities. In addition, molecular docking analysis was performed using dihydropteroate synthase as the bacterial target enzyme to explore the interactions with major phenolic compounds. LC-MS/MS analysis identified shikimic acid and chlorogenic acid as predominant in the hydromethanolic extract.

Molecular and functional modulation of mycoprotein extract from edible Trametes versicolor via pH cycling and ultrasound-enhanced transglutaminase treatment.

This study investigates the structural and functional modification of mycoprotein extract from Trametes versicolor through ultrasound (US) processing combined with either pH cycling treatment or transglutaminase (TGase) cross-linking. Mycoprotein extracts from Trametes versicolor were obtained via ultrasound-assisted extraction and subsequently freeze-dried. Five treatments were compared: pH cycling, ultrasound alone, TGase cross-linking, TGase + US, and pH cycling + US.

Examining the Contents of Pes Planus Exercise Videos and Evaluating Quality and Reliability.

ObjectivesThis study aimed to examine the content of the most-viewed pes planus exercise videos on YouTube and evaluate their quality and reliability.MethodsYouTube was searched with keywords "Pes planus exercises," "Pes planus rehabilitation," "Pes planus physiotherapy," "Flat foot exercises," "Flat foot rehabilitation," and "Flat foot physiotherapy." A total of 360 videos were independently reviewed by 2 evaluators.

The 8-Factor reasoning styles scale: development, validation, and psychometric evaluation.

Background: Building on Hacking's historical-philosophical notion of "styles of reasoning" and subsequent three-axis formalisation (Disposition, Perception, Organization), this study develops and validates the Eight-Factor Reasoning Styles Scale (8-FRSS). The instrument operationalises eight theoretically predicted styles that arise from the orthogonal intersections Empirical ↔ Hypothetical, Metaphorical ↔ Analogical, and Inductive ↔ Deductive.

Methods: Items (5 per style; 40 total) were generated from the Reasoning Style Model, vetted by five measurement experts, and refined through a pilot face-validity study (n = 50).

Taekwondo exercises for women improve quality of life, physical self-defence skills, and psychological resilience.

Background: The extant research on Taekwondo has focused primarily on the physiological effects of training, with limited interest in psychological resilience, self-defence and quality of life levels. The present study examined the effects of Taekwondo exercises on psychological resilience, self-defence and quality of life levels in healthy female subjects.

Methods: The present study comprised 30 healthy, sedentary female subjects.

Detection of canine distemper virus (CDV) infection and expression of its receptors nectin-4 and SLAM in uterine samples of dogs: can CDV cause persistent uterine infection?

Canine distemper virus (CDV) causes highly contagious, systemic, and fatal infections of domestic carnivores worldwide. The high genetic and antigenic variability of the CDV H protein and the specificity of its interaction with signaling lymphocyte activation molecule (SLAM) and nectin-4 play a crucial role in determining the host specificity and tropism of the virus. It remains to be definitely established whether CDV may be localised in the urogenital tract or cause abortions in pregnant dogs.

Evaluation of the potential protective effect of hydrogen-rich saline against brain injury in experimental head trauma model in rats.

‎Head trauma is one of the main reasons for morbidity and mortality in motor vehicle and work accidents. This study aimed to evaluate the effect of hydrogen on head trauma injury. The study groups were divided into four groups, with six rats in each group as follows: Control or no treatment (group C), Head Trauma Injury (group HI), Hydrogen-rich Saline (group H2), and Head Trauma Injury + hydrogen-rich saline (group HI+H2).

Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems.

Drought stress significantly impacts crop productivity, yet its influence on genomic and epigenetic variation in quinoa remains poorly understood. This study aimed to assess DNA damage and cytosine methylation alterations in six quinoa genotypes (Titicaca, Rainbow, Moqu Arrochilla, Cherry Vanilla, China, and White) exposed to five irrigation levels (5%, 10%, 25%, 50%, and 100% field capacity). Genomic changes were evaluated using inter-primer binding site (iPBS) markers, while DNA methylation was analyzed via CRED-iPBS.

Prediction of Mature Body Weight of Indigenous Camel () Breeds of Pakistan Using Data Mining Methods.

The determination of the live body weight of camels (required for their successful breeding) is a rather difficult task due to the problems with handling and restraining these animals. Therefore, the main aim of this study was to predict the ABW of eight indigenous camel () breeds of Pakistan (Bravhi, Kachi, Kharani, Kohi, Lassi, Makrani, Pishin, and Rodbari). Selected productive (hair production, milk yield per lactation, and lactation length) and reproductive (age of puberty, age at first breeding, gestation period, dry period, and calving interval) traits served as the predictors.

Exogenously applied putrescine and chitosan-putrescine nanocomposite alleviate the negative effects of chilling stress on iceberg lettuce seedlings.

Climate change-induced stressors such as extreme temperatures negatively affect plant growth and development. Therefore, methods are needed to improve plant tolerance to abiotic stresses. The aim of this study was to evaluate whether the foliar application of putrescine (Put) and a chitosan-putrescine nanocomposite (Ch-Put) can modulate the antioxidant response and membrane properties of lettuce under chilling stress.

The Cytotoxic Effect on HepG2 Cell Line and In Vitro, In Silico Evaluation of 1,2,4-Triazine Compounds as Inhibitors of Acetylcholinesterase and Glutathione S-Transferase.

The inhibitory effects of several 1,2,4-triazine compounds (Tr-1, Tr-2, Tr-3) on acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes were explored. IC values for 1,2,4-triazine compounds were determined, ranging from 2.45 to 9.

Chemical Profiling via LC-ESI-MS/MS and Functional Bioactivities of Foeniculum vulgare subsp. Capillaceum: Insights Into Antioxidant, Antidiabetic, and Antibacterial Potentials for Food Applications.

Driven by the increasing interest in the incorporation of naturally derived ingredients in functional foods, researchers have placed greater emphasis on the process of identifying bioactive constituents from medicinal plants, as they can provide health benefits and nutritional potential. This study offers a phytochemical profiling and bioactivities investigation conducted for the first time for an Algerian wild Foeniculum vulgare subsp. capillaceum, focusing on its potential for functional food applications.

Assessment of Eurygaster integriceps parameters to improve integrated pest management in Southeastern Türkiye wheat fields.

Cereals, as fundamental agricultural products, are a global food security concern. In Türkiye, cereals account for 50% of arable land, with wheat representing 67% of all cereal production. Sunn pest, Eurygaster integriceps (Puton) is the main pest of wheat and other cereals in Türkiye.

Prevalence of Hand Hygiene in Post-Disaster Environments and Affecting Factors: A Study on Earthquake-Affected Populations in Turkey.

Introduction: Natural disasters can increase the risk of infection by severely disrupting access to basic needs, including clean water and sanitation. Hand hygiene, one of the simplest and most effective ways to prevent infections, often becomes a challenge in such situations. The study focused on individuals living in temporary housing following the earthquakes in Turkey on February 6, 2023.

Recombinant Expression of L-methioninase from Brevibacterium linens and Evaluation of its Anticarcinogenic Properties against MiaPaCa-2 Cells.

Introduction: This study aimed to investigate the anti-carcinogenic effects of recombinant L- methioninase (rBlmet) on the pancreatic cancer cell line MiaPaCa-2.

Methods: In this study, rBlmet was initially cloned, expressed, and purified. To increase enzyme activity, the His-tags on the enzyme were removed using thrombin.

Synthesis, characterization, In vitro and In silico investigations of novel 1,2,3-triazole substituted salicylic acid phenolic hydrazones hybrids targeting TGF-β2 expression in colorectal carcinoma.

In this study, sixteen novel 1,2,3-triazole-substituted salicylic acid phenolic-hydrazone hybrids were synthesized and characterized using NMR, IR, HRMS, and HPLC techniques. The compounds were evaluated for their anticancer potential against HCT-116, Caco-2 and HT-29 colorectal carcinoma cells and normal BEAS-2B epithelial cells. Among them, compound 10k exhibited potent antiproliferative effects on HCT-116, Caco-2 and HT-29 with IC values of 6.

Effects of repetitive firings on mechanical and phase formation changes in zirconia produced by CAD-CAM and 3D printing.

Statement Of Problem: Although 3-dimensionally (3D) printed zirconia has been developed as an alternative to milled computer-aided design and computer-aided manufactured (CAD-CAM) zirconia, knowledge on the material's mechanical and phase properties, as well as how these properties change after repeated firing is lacking.

Purpose: The purpose of this in vitro study was to determine the effects of repetitive firings on the phase transformation and mechanical properties of zirconia materials produced by 2 different techniques.

Material And Methods: A total of 104 rectangular prisms (5×5×1 mm) and 8 disks (Ø6×1.

Enhancing Disaster Preparedness and Response: Key Strategies and Interventions.

Disasters, both natural and human-made, pose significant challenges to public health systems worldwide. This Research Letter examines the latest strategies and interventions in disaster preparedness and response. Our study highlights key practices that enhance the readiness and resilience of healthcare professionals and communities against disasters.

Immobilization of L-asparaginase on genipin and divinyl sulfone cross-linked multi-walled carbon nanotubes and silica supports for acrylamide mitigation.

A recombinant L-asparaginase from Geobacillus kaustophilus was immobilized onto multi-walled carbon nanotubes and silica supports using divinyl sulfone and genipin crosslinkers. The optimal pH was determined to be 8.5 for all enzyme forms, while the optimal temperature was 55 °C for the free enzyme and 60 °C for all immobilized forms.

Evaluation of In Vivo and In Vitro Toxicity of Chestnut ( Blume) Plant: Developmental Toxicity in Zebrafish Embryos Cytotoxicity, Antioxidant Activity, and Phytochemical Composition by LC-ESI-MS/MS.

The search for novel therapeutic agents has led to increasing interest in natural products, driven by the recognition that they may offer safer and more sustainable alternatives to synthetic drugs. This study aims to fill the gap in knowledge regarding the biological activity and safety of the water extract of chestnut () (chestnut), a plant species with a long history of use in traditional medicine, by conducting a comprehensive evaluation of its antioxidant, antidiabetic, and neuroprotective properties. This study presents a comprehensive analysis of the water extract of chestnut for the first time using various bioanalytical antioxidant methods.