Longitudinal changes in bodyweight, body condition, and muscle condition in ageing pet cats: findings from the Cat Prospective Ageing and Welfare Study.

Body composition metrics such as bodyweight, body condition score (BCS) and muscle condition score (MCS) can be readily recorded as part of veterinary examinations in ageing cats. However, the description of how these parameters change with age, whilst accounting for sex and age-related morbidity, is limited. The aim of this prospective cohort study was to evaluate age, sex and health-related changes in bodyweight, BCS and MCS in client-owned pet cats.

Clinical Outcomes of a Multicenter Study Following Implantation of an Isofocal Optic IOL with Double C-Loop Haptics.

Purpose: To report clinical outcomes in patients implanted with an isofocal optic-design intraocular lens (IOL) with double C-loop haptics following cataract surgery.

Methods: This was a multicentre-prospective-study involving 108 eyes (54 subjects) implanted with the Isopure Serenity (BVI, Inc). IOL.

Rapid Atrial Pacing After TAVI for Pacemaker Prediction.

Despite continued advancements in transcatheter aortic valve implantation (TAVI) techniques, the incidence of permanent pacemaker implantation (PPI) remains substantial. Established predictors of PPI include advanced age, pre-existing electrocardiographic conduction abnormalities, prosthetic valve type, implantation depth, and anatomical parameters, such as membranous septum length, which are currently under active investigation. In routine clinical practice, the management strategy often involves the temporary placement of a transvenous pacemaker lead, followed by a period of observation.

Updated Trends in Valvular Heart Disease-Related Heart Failure in G20-the Group of Twenty Countries: Insights From the Global Burden of Disease Study 2021.

Background: Valvular heart disease (VHD), including both non-rheumatic valvular heart disease (NRVHD) and rheumatic valvular heart disease (RVHD), is a major global health concern. Moreover, the progression of VHD to heart failure (HF) poses substantial clinical and public health challenges. In light of the global population aging, alongside increasing cardiovascular risk factors, and the additional strain imposed by the COVID-19 pandemic, a timely reassessment of the VHD-related HF burden is urgently needed.

Immediate and durable effects of maternal tobacco consumption on placental DNA methylation: a replication and discovery study.

An increasing number of epigenome-wide association studies report tobacco smoking-associated DNA methylation levels. However, comprehensive replication studies remain scarce, particularly in placenta, despite their crucial interest in such a large-scale context. Using DNA methylation data from the EPIC array of 341 new placentas (85 smokers, 219 non-smokers, and 37 former smokers) from the EDEN cohort, we used a candidate approach to replicate maternal smoking-associated CpGs and regions previously identified using the 450K array, and an exploratory approach to discover new associations within EPIC-specific CpGs.

Ammonia-to-Urea Ratio: A Noninvasive First-Line Tool for Detecting Clinically Significant Portal Hypertension.

Introduction: Cirrhosis progresses from compensated to decompensated phases, often marked by portal hypertension and complications like ascites, variceal hemorrhage, and hepatic encephalopathy. The ammonia-to-urea (A-to-U) ratio, reflecting urea cycle efficiency, may offer superior diagnostic performance compared to plasma ammonia levels alone. This study compared the diagnostic accuracy of the A-to-U ratio and plasma ammonia levels for identifying portal hypertension.

Transmission networks of long-term and short-term knowledge in a foraging society.

Cultural transmission across generations is key to cumulative cultural evolution. While several mechanisms-such as vertical, horizontal, and oblique transmission-have been studied for decades, how these mechanisms change across the life course, beyond childhood, remains unclear. Furthermore, it is under-explored whether different mechanisms apply to distinct learning processes: long-term learning-where individuals invest time and effort to acquire skills-and short-term learning-where individuals share information of immediate use.

A hybrid 1DCNN-GRU deep learning framework for classifying caprine granulosa cell fertility potential using single-cell transcriptomics.

Background And Aim: Granulosa cells (GCs) are crucial mediators of follicular development and oocyte competence in goats, with their gene expression profiles serving as potential biomarkers of fertility. However, the lack of a standardized, quantifiable method to assess GC quality using transcriptomic data has limited the translation of such findings into reproductive applications. This study aimed to develop a hybrid deep learning model integrating one-dimensional convolutional neural networks (1DCNNs) and gated recurrent units (GRUs) to classify GCs as fertility-supporting (FS) or non-fertility-supporting (NFS) using single-cell RNA sequencing (scRNA-seq) data.

Clonal overlap and resistance profiles of multidrug-resistant in humans and domestic animals in Brazil: A One Health molecular epidemiology study.

Background And Aim: The global rise of multidrug-resistant (MDR) poses a serious threat to human and animal health. Close proximity between humans and domestic animals may facilitate zoonotic transmission of MDR strains, underscoring the need for integrated surveillance strategies. This study aimed to investigate the genetic diversity, resistance mechanisms, and virulence gene profiles of isolates from domestic animals and humans in Mato Grosso, Brazil, within the One Health framework.

Optimizing whole-body cryostimulation exposure duration: Effects of age, sex, and body mass index in achieving target skin temperature.

The optimal Whole-body cryostimulation (WBC) exposure duration to reach the target skin temperature may vary depending on individual factors such as age, sex, and body mass index (BMI). Maintaining skin temperatures below 13.6°C is necessary to trigger significant cold-induced analgesia.

Rationally Designed InhA Inhibitors: A Comparative Anti-Tubercular Activity Study of Sulfonate Esters of Isoniazid Hydrazones and Their Structurally Flexible Benzyl Analogues.

Molecular hybridization of isoniazid with hydrophobic aromatic moieties represents a promising strategy for the development of novel anti-tubercular therapeutics. In this study, a series of hybrid molecules (5a-i) was synthesized by linking isoniazid with aromatic sulfonate esters via a hydrazone bridge. Molecular docking studies revealed that these compounds interact effectively with the catalytic triad of the InhA enzyme (Y158, F149, and K165), suggesting their potential as InhA inhibitors.

Interaction studies by NMR on the multivalent interaction between chondroitin sulfate E derivatives and the langerin receptor.

In this paper, we present the NMR analysis of multivalent compounds displaying chondroitin sulfate E (CS-E) disaccharide ligands and their interaction with langerin. The disaccharides correspond to the two alternative sequences of CS-E: GlcA-GalNAc and GalNAc-GlcA. Firstly, we studied the conformation of the two corresponding series of glycodendrimers free in solution and in the presence of langerin.

Green thermocatalytic ammonia synthesis assisted by catalytic hydrogen transfer reactions from alcohols.

This preliminary work opens a possible innovative route to investigating a paradigm shift in ammonia synthesis. It involves a hydrogen donor molecule (HDM) to favor ammonia synthesis at low pressure and temperature (1 bar, < 200 °C), subsequently reducing the environmental footprint of NH synthesis.

Assessing Severity in Anorexia Nervosa Using Alternative Criteria to the DSM-5 in a Cross-Sectional Study.

Objective: This study compared overvaluation of weight and shape (OWS), drive for thinness (DT), and their combination (OWS-DT) as alternative severity classifications for anorexia nervosa (AN) to the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) severity classification.

Method: 312 AN treatment-seeking outpatients (mean age = 26.8, SD = 10.

Evaluating the safety of a robotic supine gait training device for pediatric rehabilitation - a retrospective study.

PurposeThis study aimed to test a robotic supine gait training (RSGT) device's safety when treating children and adolescents with a variety of diagnoses, to ensure their safety and the standardization of clinical practices.MethodsThis retrospective observational study included 280 patients who underwent one or more treatment sessions with a RSGT device (DPA Med) at the Nantes Regional Children's and Adolescent Health Care Center. These patients' medical files, indexed in the digital medical file manager program, were examined in search of evidence of adverse events presumably associated with the treatment.

Ru-Catalyzed Asymmetric Transfer Hydrogenation of α-iminophosphonates: A Novel Synthetic Approach for Valuable Chiral α-Aminophosphonates.

A novel Ru-catalyzed asymmetric transfer hydrogenation (ATH) strategy has been developed for the efficient synthesis of valuable chiral α-aminophosphonates from readily available α-iminophosphonates. This method enables the conversion of both acyclic and cyclic substrates in high yields (up to 97%) and excellent enantioselectivities (up to >99:1), providing a practical entry to phosphorus-containing chiral amines. Notably, this is the first reported application of ATH to α-iminophosphonates, offering a robust and operationally convenient alternative to conventional asymmetric hydrogenation (AH) approaches.

Feasibility of mapping cross-country population coronavirus disease 2019 metrics in a federated design: learnings from a HealthData@EU Pilot use case.

The European Health Data Space aims to transform health data management across the EU, supporting both primary and secondary uses of health data while ensuring trust through General Data Protection Regulation compliance. As part of the HealthData@EU Pilot, this study investigates coronavirus disease 2019 (COVID-19) testing, vaccination, and hospitalization metrics across six European countries, with a focus on socioeconomic disparities and challenges in cross-border data access and standardization. This observational, retrospective cohort study used a federated analysis framework across Belgium, Croatia, Denmark, Finland, and France.

User journeys in cross-European secondary use of health data: insights ahead of the European Health Data Space.

The European Health Data Space (EHDS) regulation aims to facilitate cross-border sharing of health data across Europe. However, practical challenges related to data access, interoperability, quality, and interpretive competence remain, particularly when working with health systems across countries. This study aimed to evaluate and report the user journey of researchers accessing and utilizing health data across four European countries for secondary research purposes prior to implementation of EHDS.

Restoring Chloride Efflux in Cystic Fibrosis with TMEM16a Antisense Oligonucleotides.

Patients with cystic fibrosis (CF) who are non-responsive to treatments due to specific mutations need alternative CFTR-independent therapies. This study aims to assess the impact of TMEM16a expression by a specific oligonucleotide (TMEM16a ASO) on dysregulated parameters in CF, which will help prepare for preclinical studies. In this study, we analyzed the effects of TMEM16a oligonucleotide within a CF context by evaluating the impact, optimal administration route, toxicity, and specificity in primary cells and various mouse models.

Series of Microporous Redox-Active Pillared Metal-Organic Frameworks Based On Alloxazine Ligands.

Two series of robust pillared metal-organic frameworks (MOFs) are obtained under solvothermal conditions by combining a metal salt with either Hbpdc, biphenyl-4,4'-dicarboxylic acid, or Hpda, 1,4-phenylenediacrylic acid, forming 2D layers, which are pillared by L, an alloxazine derivative of 1,4-di(pyridin-4-yl)benzene using a one-pot three-component strategy. Crystallographic studies reveal the formation of two isomorphous series of compounds, namely 1-M (from Hbpdc with M = Co, Ni, Cu, and Zn) and 2-M (from Hpda with M = Co or Cu). The multifunctional compounds have high decomposition temperatures, and their sorption properties were measured, revealing relatively low surface areas.

Strengthening population and organizational health literacy to reduce social inequalities within the JA PreventNCD.

Aims: Health literacy is considered a key social determinant of health. It plays an important role in the prevention and management of non-communicable diseases (NCDs), contributing to better health and well-being. Therefore, the overall aim of the health literacy focus (Action) within the European Joint Action to prevent NCDs (JA PreventNCD) is to promote general, digital, mental and organizational health literacy to improve health outcomes and counteract NCD-related health inequities in Europe.

Hypoglycemia incidence and behavioural adjustments during free-living unstructured physical activity in adults with type 1 diabetes using AID systems: Results from the RAPPID study.

Aims: To assess the frequency and management of hypoglycaemia during unstructured physical activity (PA) in adults with type 1 diabetes (T1D) using automated insulin delivery (AID) systems in real-life settings.

Materials And Methods: RAPPID is a prospective, multicenter, observational study conducted over 1 month in four French tertiary care centres. Adults with T1D using one of three AID systems (MiniMed 780G, Tandem t:slim X2 with Control-IQ, or Ypsopump with CamAPS FX) and performing ≥2 unstructured PA sessions per week were included.

Injuries and risk factors in men's beach soccer: Japanese national championships 2013-2023.

Understanding the most common injuries in beach soccer and their risk factors is essential for ensuring player safety. We aimed to describe the injury patterns and identify factors associated with the risk of injury in men's beach soccer. We prospectively recorded injuries reported by players at an on-site aid station during the Japanese National Beach Soccer Championships between 2013 and 2023 (9 tournaments).

Impact of forward and backward walking on gait parameters across parkinson's disease stages and severity: a prospective observational study.

Background: Parkinson's disease (PD) is characterized by motor symptoms altering gait domains such as slow walking speed, reduced step and stride length, and increased double support time. Gait disturbances occur in the early, mild to moderate, and advanced stages of the disease in both backward walking (BW) and forward walking (FW), but are more pronounced in BW. At this point, however, no information is available about BW performance and disease stages specified using the Hoehn and Yahr (H&Y) scale.