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Article Abstract
Nowadays, efficient electrochemiluminescence (ECL) emitters are urgently demanded to enhance the biosensor's sensitivity. In this work, we selected Pd-based metal organic frameworks (Pd-MOFs) as nanocarrier to assemble with tris (bipyridine) ruthenium (RuP) for ECL emission, and utilized it as bio-label in ECL biosensors. Three ligands with varying amino groups were employed to regulate RuP's ECL responses, including 2-nitro-1,4-phenylenediamine (NPD), 1,2,4,5-benzenetetramine tetrahydrochloride (BTA) and hexaaminobenzene (HAB). These flexible coordination ligands allowed us to study the orbital energy levels and electroactivity of the Pd-MOFs, revealing a clear relationship between the ECL efficiency and MOFs. The efficient ECL signal transduction was achieved using the competitive recognition of RuP-Pd-HAB-linked glypican-3 (GPC) with free GPC on its aptamer assembled streptavidin magnetic beads. Our ECL biosensor showed a wide detection range, a low detection limit of 20.0 fg mL, and high clinical diagnostic accuracy in real human serum. This work expands variable regulation strategies in MOFs-based ECL emitter design and highlights novel biomarker detection potential in biochemical fields.
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