Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Cluster of Differentiation 83 (CD83) is a multifunctional regulatory molecule that has been identified in several mammals, birds, and fishes; however, no reports have described CD83 in ducks to date. Given the important role ducks play in the transmission of avian influenza viruses (AIVs), this study aimed to characterize the molecular features of duck CD83 (duCD83) and investigate its potential role during H9N2 AIV infection. We successfully cloned the coding sequence (CDS) and extracellular domain of duCD83 for the first time and expressed the recombinant protein in human embryonic kidney 293T (HEK293T) cells. The results showed that, similar to other species, duCD83 exists as a dimeric glycoprotein and is present in both membrane-bound (dumCD83) and soluble (dusCD83) forms. Sequence analysis revealed high homology between duCD83 and CD83 from swans and chickens, but lower similarity to those from mammals and fishes. Furthermore, duCD83 expression was upregulated in duck embryo fibroblasts (DEFs) following H9N2 AIV infection. Exogenous addition of dusCD83 enhanced viral replication, while knockdown of CD83 expression suppressed it. These findings suggest that duCD83 may play a role in modulating viral replication during H9N2 AIV infection.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329118 | PMC |
| http://dx.doi.org/10.1016/j.psj.2025.105570 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Panaxadiol acts as an HIF-1α inhibitor to suppress H9N2-induced inflammation.
Vet Microbiol
October 2025
School of Medicine, Shaoxing University, Shaoxing 312000, China; School of Medicine, Shanxi University of Chinese Medicine, Taiyuan 030000, China. Electronic address:
The H9N2 avian influenza virus (AIV) represents a considerable threat to both poultry industries and public health, not only due to its widespread prevalence but also because of its potential to facilitate the emergence of more virulent influenza strains through genetic reassortment. Recent studies have highlighted the pivotal role of hypoxia-inducible factor 1-alpha (HIF-1α) in viral pathogenesis, immune modulation, and the regulation of inflammatory responses, positioning it as a promising target for antiviral strategies. In this study, we identified that HIF-1α actively contributes to the inflammatory response triggered by H9N2 AIV infection in MH-S cells.
View Article and Find Full Text PDFMDCK cell line expressing H9N2 avian influenza virus NS1 protein promotes replication of the NS1 gene truncation virus.
Vet Microbiol
October 2025
College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu 225009, PR China; Joint Laboratory Safety of International Cooperation of Agricu
H9N2 subtype avian influenza virus (AIV) remains a major threat to poultry industry. Our previously developed live-attenuated vaccine candidate rTX-NS1-128(mut) demonstrated promising immunogenicity, but its truncated NS1 gene reduced replication in MDCK cells relative to the parental rTX strain. In this study, we engineered an MDCK derived cell line (2G8D5) to enhance replication of interferon-sensitive AIV candidates.
View Article and Find Full Text PDFBivalent Oral Vaccine Using Attenuated Gallinarum Delivering HA and NA-M2e Confers Dual Protection Against H9N2 Avian Influenza and Fowl Typhoid in Chickens.
Vaccines (Basel)
July 2025
College of Veterinary Medicine, Jeonbuk National University, Specialized Campus, Iksan 54596, Republic of Korea.
Fowl typhoid (FT), a septicemic infection caused by Gallinarum (SG), and H9N2 avian influenza are two economically important diseases that significantly affect the global poultry industry. We exploited the live attenuated Gallinarum (SG) mutant JOL3062 (SG: ∆ ∆ ∆) as a delivery system for H9N2 antigens to induce an immunoprotective response against both H9N2 and FT. To enhance immune protection against H9N2, a prokaryotic and eukaryotic dual expression plasmid, pJHL270, was employed.
View Article and Find Full Text PDFAvian Influenza Surveillance Among Migratory Birds, Poultry, and Humans Around Nansi Lake, China, 2021-2024.
Viruses
August 2025
The Key Laboratory of Environmental Pollution Monitoring and Disease Control, School of Public Health, Ministry of Education, Guizhou Medical University, Guiyang 550025, China.
Avian influenza A viruses (AIVs) pose a significant pandemic threat due to their cross-species transmission potential. However, AIV surveillance at the critical "migratory birds-poultry-exposed population" interface remains limited. Between 2021 and 2024, we implemented a prospective One Health surveillance program around Nansi Lake, monitoring AIVs in migratory birds, poultry, and environmental samples, as well as serological investigations against representative AIVs among migratory birds or poultry-exposed subjects.
View Article and Find Full Text PDFAn emerging PB2-627 polymorphism increases the zoonotic risk of avian influenza virus by overcoming ANP32 host restriction in mammalian and avian hosts.
J Virol
August 2025
National Key Laboratory of Veterinary Public Health Security, Key Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Alterations in the PB2-627 domain of avian influenza virus (AIV) can potentially increase the risk of cross-host species infections in humans and mammals. Recently, there has been a rise in human cases of AIV infections without the presence of the known mammalian determinant PB2-E627K. Here, we identified a variant, PB2-627V, which has evolved in poultry and has contributed to the increase in human AIV infections.
View Article and Find Full Text PDF