Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Background: High rates of misdiagnosis and missed diagnosis of schizophrenia highlight the urgent need for objective diagnostic indicators. Previous studies showed that microRNAs and circular RNAs are potential candidates. This study aimed to investigate the regulatory patterns and brain validation of circular RNAs-microRNAs-messenger RNAs in early-onset schizophrenia.
Methods: Differentially expressed circular RNAs and microRNAs were identified in blood samples from 32 patients with early-onset schizophrenia and 29 healthy controls. Circular RNAs were confirmed to possess circular structures. The interactions among circular RNAs, microRNAs, and messenger RNAs were examined using dual-luciferase reporter assays. The circRNA of interest was knocked down and overexpressed in the SH-SY5Y cell line to evaluate changes in gene expression. Brain validation of the axis was performed in animal models.
Results: Compared with healthy controls, 8 circular RNAs and 3 microRNAs exhibited significant and stable differential expression, with 6 of the 8 circular RNAs confirmed to have circular structures. Hsa-circ-0000713 was identified as containing the response element for hsa-miR-370-3p, which targeted ANK3 and MGLL. Hsa-circ-0000713 acted as a sponge, mitigating the inhibitory effects of hsa-miR-370-3p on ANK3 and MGLL. The expression patterns of this axis in the prefrontal cortex were consistent with those observed in peripheral blood samples from clinical patients.
Conclusion: This study identified a specific multi-molecular axis in early-onset schizophrenia, wherein hsa-circ-0000713 functioned as a sponge to reduce the inhibitory effects of hsa-miR-370-3p on ANK3 and MGLL. This axis may contribute to the pathogenesis of early-onset schizophrenia by mediating abnormalities in prefrontal cortex development.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12301123 | PMC |
| http://dx.doi.org/10.2147/NDT.S521634 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Circ_0000437 promotes proliferation, invasion, migration and epithelial-mesenchymal transition of breast cancer cells by targeting the let-7b-5p/CTPS1 axis].
Nan Fang Yi Ke Da Xue Xue Bao
August 2025
College of Laboratory Medicine, Wannan Medical College, Wuhu 241000, China.
Objectives: To investigate the role of circular RNA circ_0000437 in regulating biological behaviors of breast cancer cells and the molecular mechanism.
Methods: Breast cancer MCF-7 and MDA-MB-231 cells were transfected with sh-circ_0000437, mimics, inhibitor, si-CTPS1, or their respective negative controls. qRT-PCR was used to detect the expression levels of circ_0000437, let-7b-5p, CTPS1, Notch1, Hes1, and Numb in breast cancer cell lines and tissues.
CircTTC3 regulates the osteogenic differentiation of adipose-derived mesenchymal stem cells via miR-205/Smad3 axis.
Exp Cell Res
September 2025
State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu City 610041, China. Electronic address:
Adipose-derived mesenchymal stem cells (ADSCs) hold great promise for bone tissue repair and regeneration. Circular RNAs (circRNAs) play a crucial role in regulating the osteogenic differentiation and bone remodeling of ADSCs; however, the underlying molecular mechanisms remain unclear. In this study, we conducted whole transcriptome sequencing (WTS) on ADSCs and constructed a competing endogenous RNA (ceRNA) regulatory network to identify the circTTC3/miR-205/mothers against decapentaplegic homolog 3 (Smad3) signaling axis.
View Article and Find Full Text PDFStructural Dynamics of Dengue Virus UTRs and Their Cyclization.
Biophys J
September 2025
Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Drive, Lethbridge, AB, T1K 3M4, Canada; Li Ka Shing Institute of Virology, University of Alberta, Edmonton T6G 2E1, Alberta, Canada; Department of Microbiology, Immunology
The dengue virus (DENV) poses a significant threat to human health, accounting for approximately 400 million infections each year. Its genome features a circular structure that facilitates replication through long-range RNA-RNA interactions, utilizing cyclization sequences located in the untranslated regions (UTRs). To gain new insights into the organization of the DENV genome, we purified the 5' and 3' UTRs of DENV in vitro and examined their structural and binding properties using various biophysical techniques combined with computational methods.
View Article and Find Full Text PDFPolymorphic RNA-Templated Modulation of Photophysics and Dynamics of a Molecular Rotor with Contrasting Affinity: A Demonstration of Sequential Energy Transfer-Triggered Bright White Light Generation.
Langmuir
September 2025
Biophysical Chemistry Laboratory, Physical Chemistry Section, Department of Chemistry, Jadavpur University, Raja S. C. Mullick Road, Jadavpur, Kolkata 700032, India.
Photophysical studies on the interaction of small molecules with various forms of nucleic acids are attracting attention nowadays in order to delineate the molecular level mechanism of various biological processes occurring in vivo. Herein, we employed vivid steady-state and time-resolved spectroscopic techniques to elucidate the detailed characterization of the binding interaction of a biologically active cationic dye thioflavin T (ThT) with double and triple helical forms of RNA - A.U duplex and U.
View Article and Find Full Text PDFCircSHPRH inhibits malignancy progression of head and neck squamous cell carcinoma by regulating PI3K/AKT/mTOR signaling pathway.
Funct Integr Genomics
September 2025
Department of Otolaryngology-Head and Neck Surgery, Maternal and Child Health Hospital of Hubei Province, NO.745 Wuluo Road, Hongshan District, Wuhan, 430070, Hubei Province, China.
Background: Head and neck squamous cell carcinoma (HNSCC) stands as a significant global health concern, marked by its substantial impact on both morbidity and mortality rates. Although previous studies have suggested that circular RNAs (circRNAs) may influence HNSCC progression, the underlying mechanisms remain largely unclear.
Methods: In this study, we first used quantitative real-time polymerase chain reaction (qRT-PCR) to measure the expression levels of circSHPRH in HNSCC tissues and cell lines.