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Viscosity, as an important micro-environment parameter, is closely related to the occurrence and progression of various diseases. Herein, we have developed a novel fluorescent probe (BIB) for lysosomal viscosity detection. Interestingly, using BIB, imaging of viscosity fluctuations in tumoral ferroptosis and rheumatoid arthritis models has been successfully achieved.
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http://dx.doi.org/10.1039/d5cc03040d | DOI Listing |
J Fluoresc
September 2025
School of Intelligent Manufacturing, Huzhou College, Huzhou, 313000, P.R. China.
The antibiotic contamination in aquatic environments, particularly in aquaculture systems, poses substantial risks to ecological balance and human health. To address this issue, we engineered a novel ratiometric fluorescent probe utilizing dual-emission carbon dots (D-CDs) synthesized from sustainable biomass carrot and nitrogen-rich precursors (melamine and o-phenylenediamine) through an efficient one-pot hydrothermal approach. The D-CDs exhibited dual emission peaks at 425nm and 540 nm under 370nm excitation.
View Article and Find Full Text PDFAnal Bioanal Chem
September 2025
Hebei Key Laboratory of Public Health Safety, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Public Health, College of Chemistry and Materials Science, Hebei University, Baoding, 071002, China.
This work presents the development of a highly sensitive, selective, and efficient aptamer-based fluorescent sensor for detecting cortisol in human urine. Carbon quantum dots-nucleic acid aptamer (CQDs-Apt) synthesized with excellent photoluminescent properties and stability, were selected as the fluorescent probe. In the presence of MoS-NSs, CQDs-Apt adsorbed onto the surface of MoS-NSs via electrostatic and π-π interactions, leading to strong and rapid fluorescence quenching due to static quenching mechanism between them.
View Article and Find Full Text PDFChem Asian J
September 2025
School of Chemistry and Chemical Engineering, Hainan University, Haikou, 570228, China.
Molecules that exhibit excited-state intramolecular proton transfer (ESIPT) have demonstrated great promise in fluorescent probes. The electronic effect of substituents has an important influence on the ESIPT process. In this study, we investigated the effects of substituents on the ESIPT mechanism and the photophysical behavior of single-benzene fluorophore (SBF) derivatives with computational chemistry methods.
View Article and Find Full Text PDFChemistry
September 2025
International School for Optoelectronic Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China.
Alzheimer's disease (AD) is a neurodegenerative disease characterized by β-amyloid (Aβ) deposition, imposing significant social and economic burdens globally. Despite extensive efforts have been devoted to developing fluorescent probes for Aβ imaging, further improving the luminescent efficiency of prevailing probes still remains a significant challenge. Herein, we investigated the inner mechanism of constructing high-efficient Aβ probes via a structural cyclization strategy.
View Article and Find Full Text PDFInt J Nanomedicine
September 2025
Department of Orthopedics, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.
Peptide-based fluorescent probes have found widespread applications in biomedical research, including bio-imaging, disease diagnosis, drug discovery, and image-guided surgery. Their favorable properties-such as small molecular size, low toxicity, minimal immunogenicity, and high targeting specificity-have contributed to their growing utility in both basic research and translational medicine. This review provides a comprehensive overview of recent advances in peptide-based fluorescent probes, emphasizing design strategies, biological targets, and diverse functional applications.
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