Article Synopsis
- Paralogous transcription factors (TFs) often bind to similar DNA motifs, but their homodimerization allows them to be distinguished by different configurations and specificity.
- Using high-throughput SELEX to analyze 40 R2R3-MYB TFs, researchers identified 833 DNA motif models and highlighted how homodimerization alters the binding specificity of these factors.
- Specifically, AtMYB2 was shown to gain the ability to recognize unique regulatory sequences due to its homodimerization, suggesting an evolutionary conserved mechanism that helps related TFs target distinct regulatory sites.
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Article Abstract
Paralogous transcription factors (TFs) frequently recognize highly similar DNA motifs. Homodimerization can help distinguish them according to their different dimeric configurations. Here, by studying R2R3-MYB TFs, we show that homodimerization can also directly change the recognized DNA motifs to distinguish between similar TFs. By high-throughput SELEX, we profiled the specificity landscape for 40 R2R3-MYBs of subfamily VIII and curated 833 motif models. The dimeric models show that homodimeric binding has evoked specificity changes for AtMYBs. Focusing on AtMYB2 as an example, we show that homodimerization has modified its specificity and allowed it to recognize additional regulatory sequences that are different from the closely related CCWAA-box AtMYBs and are unique among all AtMYBs. Genomic sites described by the modified dimeric specificities of AtMYB2 are conserved in evolution and involved in AtMYB2-specific transcriptional activation. Collectively, this study provides rich data on sequence preferences of VIII R2R3-MYBs and suggests an alternative mechanism that guides closely related TFs to respective regulatory sites.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11995187 | PMC |
| http://dx.doi.org/10.1002/imt2.70009 | DOI Listing |
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