Publications by authors named "Dingkun Jiang"
Article Synopsis
- Paralogous transcription factors (TFs) often bind to similar DNA motifs, but their homodimerization allows them to be distinguished by different configurations and specificity.
- Using high-throughput SELEX to analyze 40 R2R3-MYB TFs, researchers identified 833 DNA motif models and highlighted how homodimerization alters the binding specificity of these factors.
- Specifically, AtMYB2 was shown to gain the ability to recognize unique regulatory sequences due to its homodimerization, suggesting an evolutionary conserved mechanism that helps related TFs target distinct regulatory sites.
Given the key role of energy grasses in biomass energy, electricity, biofuels, and carbon sequestration, the Energy Grass Omics Database (EGDB) integrates germplasm data with genomics, transcriptomics, epigenomics, and phenomics data to support functional genomic research on diverse energy grass species. EGDB also currently supplies the largest epigenetic data set of energy grasses: a high-resolution chromatin modification, chromatin accessibility, and gene expression landscape of pearl millet to provide insights into regulatory traits essential for sustainable energy production.
View Article and Find Full Text PDFThe Arabidopsis transcription factor WUSCHEL-related homeobox 14 (AtWOX14) plays versatile roles in plant growth and development. However, its biochemical specificity of DNA binding, its genome-wide regulatory targets, and how these are affected by DNA methylation remain uncharacterized. To clarify the biochemistry underlying the regulatory function of AtWOX14, using the recently developed 5mC-incorporation strategy, this study performed SELEX and DAP-seq for AtWOX14 both in the presence and absence of cytosine methylation, systematically curated 65 motif models and identified 51,039 genomic binding sites for AtWOX14, and examined how 5mC affects DNA binding of AtWOX14 through bioinformatic analyses.
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