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Correction for 'Strategies for the detection of site-specific DNA methylation and its application, opportunities and challenges in the field of electrochemical biosensors' by Chenliu Ye , , 2024, , 5496-5508, https://doi.org/10.1039/D4AY00779D.
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http://dx.doi.org/10.1039/d5ay90024g | DOI Listing |
Nat Metab
September 2025
Cellular and Molecular Physiology Department, Yale School of Medicine, New Haven, CT, USA.
The essential cofactor coenzyme A (CoASH) and its thioester derivatives (acyl-CoAs) have pivotal roles in cellular metabolism. However, the mechanism by which different acyl-CoAs are accurately partitioned into different subcellular compartments to support site-specific reactions, and the physiological impact of such compartmentalization, remain poorly understood. Here, we report an optimized liquid chromatography-mass spectrometry-based pan-chain acyl-CoA extraction and profiling method that enables a robust detection of 33 cellular and 23 mitochondrial acyl-CoAs from cultured human cells.
View Article and Find Full Text PDFAnal Chem
September 2025
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, P. R. China.
Electrogenerated chemiluminescence (ECL) methods have been widely used in clinical diagnosis. Although ECL peptide-based biosensors continue to grow with good sensitivity and signal flexibility, little emphasis has been placed on the effect of the peptide sequence on ECL sensitivity. We herein studied the nuanced effects of different peptide sequences on the analytical performance of ECL peptide-based biosensors for matrix metalloproteinase 2 (MMP-2) assay, in which [(pbz)Ir(DMSO)Cl] (pbz = 3-(2-pyridyl)benzoic acid) was used as the ECL emitter while a specific peptide was used as the molecular recognition element.
View Article and Find Full Text PDFBiosaf Health
August 2025
NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
The epidemiological characteristics of emerging infectious disease outbreaks in recent years have underscored the critical importance of controlling imported infectious diseases. In this study, we implemented dynamic tracking of microbial invasions by monitoring environmental microbes at the customs and ports. From July to September 2024, a total of 126 environmental samples were collected from three ports of entry in Shenzhen, China.
View Article and Find Full Text PDFWater Res
August 2025
Department of Civil and Environmental Engineering, Pusan National University, Busan 46241, Republic of Korea; Institute for Environment and Energy, Pusan National University, Busan 46241, Republic of Korea. Electronic address:
Wastewater-based epidemiology (WBE) is increasingly used as a complementary tool for monitoring drug use at the population level, providing anonymized, real-time estimates of community drug consumption. Site-specific applications of WBE can identify localized patterns that national or municipal surveys may overlook. This study presents the first comprehensive, site-specific assessment of illicit drug use in South Korea using WBE.
View Article and Find Full Text PDFTalanta
August 2025
School of Public Health &Jiangxi Provincial Key Laboratory of Disease Prevention and Public Health, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, 330031, PR China. Electronic address:
Hemorrhagic fever with renal syndrome (HFRS), caused by Hantaan virus, poses a serious public health threat. Current diagnostic methods remain limited by low sensitivity, complex procedures, and high sample requirements. To address this, we developed a highly sensitive single-molecule biosensor using multi-fluorophore nucleic acid probes and STORM imaging for the detection of Hantaan virus RNA.
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