Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The large-scale loach (Paramisgurnus dabryanus; Cypriniformes: Cobitidae) is primarily distributed in East Asia. It is an important economic fish species characterized by fast growth, temperature-dependent sex determination and the ability to breathe air. Currently, molecular mechanism studies related to some aspects such as sex determination, toxicology, feed nutrition, growth and genetic evolution have been conducted. However, the lack of a high-quality reference genome has hindered further research. In this study, we performed PacBio HiFi and Hi-C sequencing on a female (ZW) specimen and assembled the first high-quality chromosome-level genome of the large-scale loach. The assembled genome comprises 24 chromosomes, with a total length of 1.04 Gb, a scaffold N50 of 41.7 Mb, and a BUSCO completeness of 95.8%, including 28,311 protein-coding genes. These findings not only provide new insights into the genome structure of the large-scale loach but also establish a crucial reference point for omics studies and serve as an essential genomic resource for breeding programs in this species.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770070 | PMC |
| http://dx.doi.org/10.1038/s41597-025-04498-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Identifying in vivo genetic dependencies of melanocyte and melanoma development.
Elife
August 2025
Department of Cancer Biology and Genetics, Memorial Sloan Kettering Cancer Center, New York, United States.
The advent of large-scale sequencing in both development and disease has identified large numbers of candidate genes that may be linked to important phenotypes. We have developed a rapid, scalable system for assessing the role of candidate genes using zebrafish. We generated transgenic zebrafish in which Cas9 was knocked in to the endogenous locus, a master transcription factor of the melanocyte lineage.
View Article and Find Full Text PDFA systems genetics approach identifies roles for proteasome factors in heart development and congenital heart defects.
PLoS Genet
August 2025
Center for Developmental Biology and Regenerative Medicine, Seattle Children's Research Institute, Seattle, Washington, United States of America.
Congenital heart defects (CHDs) occur in about 1% of live births and are the leading cause of infant death due to birth defects. While there have been remarkable efforts to pursue large-scale whole-exome and genome sequencing studies on CHD patient cohorts, it is estimated that these approaches have thus far accounted for only about 50% of the genetic contribution to CHDs. We sought to take a new approach to identify genetic causes of CHDs.
View Article and Find Full Text PDFCapturing nematic order on tissue surfaces of arbitrary geometry.
Nat Commun
August 2025
School of Physics, UNSW, Sydney, NSW, Australia.
A leading paradigm for understanding the large-scale behavior of tissues is via generalizations of liquid crystal physics; much like liquid crystals, tissues combine fluid-like, viscoelastic behaviors with local orientational order, such as nematic symmetry. Whilst aspects of quantitative agreement have been achieved for flat monolayers, the most striking features of tissue morphogenesis-including symmetry breaking, folding and invagination-concern surfaces with complex curved geometries in three dimensions. As yet, however, characterizing such behaviors has been frustrated due to the absence of proper image analysis methods; current state-of-the-art methods almost exclusively rely on two-dimensional intensity projections of multiple image planes, which superimpose data and lose geometric information that can be crucial.
View Article and Find Full Text PDFIdentification and functional analysis of a novel potent anti-angiogenesis peptide SAIF-B2 derived from shark cartilage.
Eur J Pharmacol
October 2025
Institute of Biomedicine & Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China; State Key Laboratory of Bioactive Molecules and Druggability Assessment, Jinan University, Guangzhou, 510632, China; National Engineering Research Center of Gene
Marine-derived bioactive molecules represent promising candidates for lead drug development. Structural optimisation of these lead drugs significantly enhances their suitability as drugs, setting the framework for large-scale manufacturing. We previously identified shark-derived angiogenesis inhibition factor (SAIF), a 33 amino acid peptide from shark cartilage, which has anti-angiogenic activity but has inadequate in vivo stability, thereby limiting clinical translation.
View Article and Find Full Text PDFStructural and genetic determinants of zebrafish functional brain networks.
Sci Adv
July 2025
Centre de recherche CERVO, Québec (Québec), Canada.
Network science has revealed universal brain connectivity principles across species. However, several macroscopic network features established in human neuroimaging studies remain underexplored at cellular scales in small animal models. Here, we use whole-brain calcium imaging in larval zebrafish to investigate the structural and genetic basis of functional brain networks.
View Article and Find Full Text PDF