Colorimetric and fluorescent probe assisted by smartphone app for monitoring fish freshness.

Food Chem

College of Food Science and Engineering, College of Chemistry and Materials Engineering, Institute of Ocean, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Bohai University, Jinzhou 121013, PR China

Published: May 2025


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Article Abstract

In this study, a novel "OFF-ON" fluorescent probe MPZ ((E)-5-((10-ethyl-2-methoxy-10H-phenothiazin-3-yl)methylene)thiazolidine-2,4-dione) based on phenothiazine is synthesized, which can rapidly (7 s) detect biogenic amines (BAs) through deprotonation, utilizing both colorimetric and fluorescent dual channels. An app for visual portable detection of fish freshness, named "Visual Evaluation", is independently developed. This app integrates several functions, including image capture, editable scanning of red, green, and blue (RGB) values, data analysis fitting, data storage, and verification. More importantly, the smart detection platform composed of the app and MPZ-loaded filter paper tags (MPZ/FPS) can quantitatively detect the total volatile basic nitrogen (TVB-N) value in salmon and has been validated for its accuracy and reliability using the national standard method of China. This smart detection platform is expected to provide new ideas for the field of portable visual inspection, and also offer merchants, and regulators a new instrument-free and intelligent reading tool for assessing the freshness of salmon.

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http://dx.doi.org/10.1016/j.foodchem.2025.143013DOI Listing

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