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In this study, a novel "OFF-ON" fluorescent probe MPZ ((E)-5-((10-ethyl-2-methoxy-10H-phenothiazin-3-yl)methylene)thiazolidine-2,4-dione) based on phenothiazine is synthesized, which can rapidly (7 s) detect biogenic amines (BAs) through deprotonation, utilizing both colorimetric and fluorescent dual channels. An app for visual portable detection of fish freshness, named "Visual Evaluation", is independently developed. This app integrates several functions, including image capture, editable scanning of red, green, and blue (RGB) values, data analysis fitting, data storage, and verification. More importantly, the smart detection platform composed of the app and MPZ-loaded filter paper tags (MPZ/FPS) can quantitatively detect the total volatile basic nitrogen (TVB-N) value in salmon and has been validated for its accuracy and reliability using the national standard method of China. This smart detection platform is expected to provide new ideas for the field of portable visual inspection, and also offer merchants, and regulators a new instrument-free and intelligent reading tool for assessing the freshness of salmon.
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http://dx.doi.org/10.1016/j.foodchem.2025.143013 | DOI Listing |
Chemistry
September 2025
Institute for Advanced Study, Shenzhen University, Shenzhen, 518060, P. R. China.
The long-term visualization of intracellular Fe dynamics and lysosomal activity is crucial for investigating the physiological roles and functions of lysosomes during the growth of organisms. The lysosome-targeted fluorescent probe (RBH-EdC), derived from rhodamine-nucleoside conjugates, demonstrates a sophisticated dual-activation design: one is Fe⁺ response, triggering spirolactam ring-opening to form xanthine structures, resulting in ≥ 1000-fold fluorescence enhancement with visible colorimetric transition (colorless→pink). Another is pH sensitivity, demonstrating protonation-dependent fluorescence amplification at the dC at site N3 (pK= 2.
View Article and Find Full Text PDFMikrochim Acta
September 2025
Department of Surgical Oncology, Shaanxi Provincial People's Hospital, 256 Friendship West Road, Beilin District, Xi'an, 710068, Shaanxi, China.
Mycoplasma pneumonia, a primary aetiological agent of atypical pneumonia, necessitates the implementation of rapid point-of-care diagnostics. Lateral flow immunoassays (LFIAs) hold promise for point-of-care testing (POCT), yet their sensitivity levels are frequently constrained by probe affinity and matrix interference. We introduce an orientational labelling strategy that employs magnetic nanoparticles (MNPs) functionalized with staphylococcal protein A (SPA) to simultaneously enhance antibody orientation and facilitate magnetic enrichment.
View Article and Find Full Text PDFMikrochim Acta
September 2025
Department of Analytical Chemistry, China Pharmaceutical University, 24 TongJiaXiang, Nanjing, 210009, Jiangsu, China.
A nanozyme-mediated cascade reaction system for fluorometric and colorimetric dual-mode detection of sarcosine (SA) was developed. The nanozymes (Zn-Glu@Hemin) were synthesized via a rapid self-assembly within 10 min at room temperature. Importantly, the Zn-Glu@Hemin exhibited strong peroxidase (POD)-mimicking activity, catalyzing the generation of hydroxyl radical (·OH) and superoxide anion (O) from hydrogen peroxide (HO), enhancing the fluorescence reaction of o-phenylenediamine (OPD) and the colorimetric reaction of 3,3',5,5'-tetramethylbenzidine (TMB).
View Article and Find Full Text PDFAnal Chim Acta
November 2025
Guangxi Key Laboratory of Natural Polymer Chemistry and Physics, Key Laboratory of Nanobiosensor Analysis, College of Chemistry and Materials, Nanning Normal University, Nanning, 530001, PR China. Electronic address:
Background: Hexavalent chromium ions (Cr(VI)), a notorious toxic heavy metal pollutant with proven carcinogenicity, endangers human health and the environment. Meanwhile, l-ascorbic acid (L-AA), a vital biological antioxidant, has abnormal levels closely tied to various diseases. Developing efficient synchronous detection methods for these two key analytes is of great value in clinical and environmental monitoring.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Key Laboratory of Jianghuai Agricultural Product Fine Processing and Resource Utilization of Ministry of Agriculture and Rural Affairs, College of Food and Nutrition, Anhui Agricultural University, Hefei, 230036, China. Electronic address:
In this study, aloe emodin and β-d-glucose pentaacetate were added into potato starch/polyvinyl alcohol for the construction of colorimetric/fluorescent dual-pass intelligent response labels. Inspired by the lotus leaf structure, retained the advantages of the label itself and solved the hydrophilic problem of the label, and further developed a multi-functional dual-channel smart label with hydrophobicity and self-adhesion. The water contact angle of the prepared T-AEB label was 120.
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