Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Penicillin G (PG) is a common antibiotic, and its accumulation in the environment can pose a threat to the ecological system and ultimately impact human health. Nanozymes have emerged as highly stable enzyme mimics that can be utilized as sensors to achieve the sensitive detection of specific antibiotics. Herein, we report on a dual single-atom Fe/Zn nanozyme (DSAzyme) synthesized from Fe-imidazole as the guest and zeolite imidazole framework-8 as the host. The DSAzyme exhibits intriguing properties that mimic the activities of two natural enzymes: peroxidase and lactamase. Both activities are utilized for the design of a colorimetric sensor for the specific detection of PG: the peroxidase activity enables color generation from 3,3',5,5'-tetramethylbenzidine and HO, and the lactamase activity provides the recognition of PG. The nanozyme consists of many Fe-N and Zn-N site and mechanistic characterizations by experimental investigations and theoretical calculations identify Fe-N as the main active center for the peroxidase activity and Zn-N as the main binding site for PG. The sensor can achieve a limit of detection of 47 nM, is able to detect PG from real-life samples, remains fully functional after 8-month storage, and retain high activities after reuse for fives times. Taken together, our study provides a new approach to the detection of antibiotics in environmental samples.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.analchem.4c02203 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Kinetic and Mechanistic Discrepancies of Single/Dual-Atom Nanozymes Drive a Triple-Channel Sensing Array for Machine Learning-Assisted Antioxidant Discrimination.
Anal Chem
September 2025
Anhui Key Laboratory of Biomedical Materials and Chemical Measurement, Key Laboratory of Functional Molecular Solids, Ministry of Education, Anhui Key Laboratory of Molecule-Based Materials, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241002, P.R. China.
Current colorimetric sensing arrays for antioxidant detection often struggle with discrimination due to cross-reactive signals from individual nanozymes. These signals are typically modulated by external factors such as pH or chromogenic substrates, offering limited kinetic and mechanistic diversity. To overcome this, we present a novel triple-channel colorimetric sensing array utilizing two distinct single-atom nanozymes (Cu SA and Fe SA) and one dual-atom nanozyme (CuFe DA).
View Article and Find Full Text PDFAxial engineering of bilayer single-atom catalysts for enhanced bifunctional oxygen electrocatalysis.
Phys Chem Chem Phys
September 2025
Institute of Materials Intelligent Technology, Liaoning Academy of Materials, Shenyang 110004, China.
Axial ligand engineering is a promising strategy to enhance the performance of single-atom catalysts (SACs) in electrocatalysis. However, a single non-metallic axial coordination atom linked to monolayer SACs (MSACs) often exhibits insufficient stability. In this work, we designed a series of bilayer SACs (BSACs) with vertically stacked FeN and MN (M = Sc-Zn) layers bridged by axial non-metallic atoms (C, N, O, P, S, and Se).
View Article and Find Full Text PDFElectrochemistry-Accelerated Water-Gas Shift Reaction on IrN-RhN Dual Sites Catalysts for Efficient Hydrogen Production.
Angew Chem Int Ed Engl
September 2025
State Key Laboratory of Chemo and Biosensing, College of Chemistry and Chemical Engineering, International Joint Lab of Energy Electrochemistry of the Ministry of Education, Hunan University, Changsha, 410082, China.
The water-gas shift reaction (WGSR) is crucial to the hydrogen economy, which is hampered by the harsh conditions and complicated purification process. In this work, the spatially separated efficient CO conversion and high-purity H production are realized by electrochemistry-accelerated water-gas shift reaction (WGSR) with IrN-RhN dual sites single atom catalysts (IrRh-NC) in high-temperature polymer-electrolyte-membrane electrolyzer. In this reaction, the Ir single atoms in the catalysts can rapidly dissociate HO at an extremely low potential to supply abundant *OH, which ensures the *OH groups bind to the spontaneously adsorbed *CO on neighboring Rh sites to further accelerate CO conversion.
View Article and Find Full Text PDFAtomic Engineering of a FeFe Dual Single-Atom Nanozyme for Enhanced Peroxidase-like Activities To Build Chemical Tongue for Discrimination of Aromatic Amines.
Anal Chem
September 2025
Department of Chemistry, Capital Normal University, Beijing 100048, China.
Single-atom nanozymes have made important progress in the field of sensors, but their catalytic performance as natural enzyme substitutes is far from satisfactory. We describe here a FeFe dual single-atom nanozyme (FeNCN) with a Fe loading of 0.89 wt %, and it shows a synergistic effect and a peroxidase (POD)-like activity.
View Article and Find Full Text PDFLight modulating Ni electronic structure to create dual single-atom Ni and Ni on ZnInS for enhanced photoreduction of CO.
J Colloid Interface Sci
August 2025
State Key Laboratory of Photocatalysis on Energy and Environment, Fuzhou University, Fuzhou, Fujian 350116, China.
Constructing dual single-atom catalysts with distinct electronic structures holds significance for the design of catalytic active centers, yet it remains highly challenging. Here, a novel light-induced approach was created to construct Ni and Ni dual single-atom sites on ZnIn₂S₄ nanosheets (Ni-Ni/ZIS) for the photocatalytic reduction of CO₂. Characterizations and density functional theory (DFT) calculations results indicate that Ni and Ni single-atom sites can be selectively anchored in the Zn vacancies and lattice interstitials on the surface of ZIS, respectively.
View Article and Find Full Text PDF