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The gene encoding 9-cis-epoxycarotenoid dioxygenase 3 () functions in abscisic acid (ABA) biosynthesis, plant growth and development, and tolerance to adverse temperatures, drought and saline conditions. In this study, three rice lines were used to explore the function of , these included an OsNCED3-overexpressing line (), a knockdown line () and wild-type rice (WT). These rice lines were infested with the brown plant hopper (BPH; ) and examined for physiological and biochemical changes, hormone content, and defense gene expression. The results showed that activated rice defense mechanisms, which led to an increased defense enzyme activity of superoxide dismutase, peroxidase, and polyphenol oxidase. The overexpression of decreased the number of planthoppers and reduced oviposition and BPH hatching rates. Furthermore, the overexpression of increased the concentrations of jasmonic acid, jasmonyl-isoleucine and ABA relative to WT rice and the line. These results indicate that improved the stress tolerance in rice and support a role for both jasmonates and ABA as defense compounds in the rice-BPH interaction.
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http://dx.doi.org/10.3389/fpls.2024.1359315 | DOI Listing |
3 Biotech
October 2025
ICAR-National Rice Research Institute, Cuttack, Odisha 753006 India.
Just as Gregor Mendel's laws of inheritance laid the foundation for modern genetics, the emergence of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas systems has catalyzed a new era in precision genome engineering. CRISPR/Cas has revolutionized rice ( L.) breeding by enabling precise, transgene-free edits to improve yield, nutrition, and stress tolerance.
View Article and Find Full Text PDFPlant Physiol Biochem
August 2025
Institute of Rice Industry Technology Research, Key Laboratory of Functional Agriculture Of Guizhou Provincial Department of Education, Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province, Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountain
Amino acids, which serve as the main source of organic nitrogen, are typically transported within plants via amino acid transporter proteins. In this research, we discovered haplotype variations in the promoter sequence of amino acid transporter OsAAP8 among rice germplasm resources. Notably, we observed that OsAAP8 expression was significantly elevated in indica rice varieties.
View Article and Find Full Text PDFPlant Physiol Biochem
August 2025
College of Resource, Sichuan Agricultural University, 211 Huimin Road, Chengdu, Sichuan, 611130, China. Electronic address:
Rice (Oryza sativa L.) is one of the most important food crops worldwide, with a strong capacity for Cd accumulation. Cd accumulation in rice is regulated by multiple genes.
View Article and Find Full Text PDFBiology (Basel)
July 2025
College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.
GOLDEN2-LIKEs (GLKs) are important transcription factors for the chloroplast development influencing photosynthesis, nutrition, senescence, and stress response in plants. Sunflower () is a highly photosynthetic plant; here, a -homologues gene was identified from the sunflower genome by bioinformatics. To analyze the bio-function of , transgenic rice plants overexpressing () were constructed and characterized via phenotype.
View Article and Find Full Text PDFPlanta
September 2025
National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, 54874, Republic of Korea.
OsWRKY113 functions as a negative regulator of rice immunity against Fusarium fujikuroi by attenuating jasmonic acid-mediated immune responses, unveiling its previously uncharacterized role within the OsWRKY transcription factor family. The fungal pathogen Fusarium fujikuroi is a major causal agent of bakanae disease in rice (Oryza sativa), a globally important staple crop. WRKY transcription factors are key regulators in the interaction between rice and this pathogen.
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