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In this study, high internal phase Pickering emulsions (HIPPEs) were stabilized by the complexes of peanut protein isolate (PPI) and cellulose nanocrystals (CNCs) for encapsulation β-carotene to retard its degradation during processing and storage. CNCs were prepared by HSO hydrolysis (HCNCs), APS oxidation (ACNCs) and TEMPO oxidation (TCNCs), exhibiting needle-like or rod-like structures with nanoscale size and uniformly distributed around the spherical PPI particle, which enhanced the emulsifying capability of PPI. Results of optical micrographs and droplet size measurement showed that Pickering emulsions stabilized by PPI/ACNCs complexes exhibited the most excellent stability after 30 days of storage, which indicated that ACNCs had the most obvious effect to improve emulsifying capability of PPI. HIPPEs encapsulated β-carotene (βc-HIPPEs) were stabilized by PPI/ACNCs complexes and showed excellent inverted storage stability. Moreover, βc-HIPPEs exhibited typical shear thinning behavior investigated by rheological properties analysis. During thermal treatment, ultraviolet radiation and oxidation, the retentions of β-carotene encapsulated in HIPPEs were improved significantly. This research holds promise in expanding Pickering emulsions stabilized by proteins-polysaccharide particles to delivery systems for hydrophobic bioactive compounds.
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http://dx.doi.org/10.1016/j.ijbiomac.2024.131196 | DOI Listing |
Food Res Int
November 2025
Hainan University-HSF/LWL Collaborative Innovation Laboratory, College of Food Sciences & Engineering, Hainan University, 58 People Road, Haikou 570228, China; Haikou Key Laboratory of Special Foods, Haikou, Hainan 570228, China.
In this study, we explored the application of lactoferrin-(-)-epigallocatechin-3-gallate (LF-EGCG) complex with rapeseed, soybean, walnut, peanut and sesame oil for the preparation of Pickering emulsions and its spray-dried microcapsules. Spectroscopy and molecular docking revealed that LF-EGCG binds via hydrogen bonds, hydrophobic interactions, and van der Waals forces. Structural analysis demonstrated that 0.
View Article and Find Full Text PDFLangmuir
September 2025
Process Engineering in Life Science Engineering, HTW Berlin, Wilhelminenhofstraße 75 A, 12459 Berlin, Germany.
Pickering emulsions (PEs), where water-in-oil (w/o) droplets are stabilized by nanoparticles (NPs), offer a promising platform for biocatalysis by providing a large interfacial area crucial for efficient substrate conversion. While several lipase catalyzed reactions in PEs have been demonstrated, the exact interfacial structure is unknown. This study focuses on the interfacial network formed by NPs and lipase (CRL) at the octanol/water-interface by varying pH and NP charge.
View Article and Find Full Text PDFACS Appl Mater Interfaces
September 2025
Institute of Postharvest and Food Sciences, Agricultural Research Organization (ARO), Volcani Center, 68 HaMaccabim Road, Rishon Letzion 7505101, Israel.
Cultivating fat for edible tissue presents significant challenges, due to the high costs associated with growth and differentiation factors, alongside the poor viability of adipocytes resulting from cell clustering. Additionally, there is a gap in research regarding the rapid accumulation of fats within cells. To that end, this study presents the development of a biodegradable soy protein colloidosome system for an efficient application: direct delivery of oils into bovine satellite cells, enabling rapid intracellular fat accumulation without the need for adipogenic differentiation.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, 201418, China. Electronic address:
Fresh walnuts are prone to moisture loss and spoilage after harvest, leading to reduced appearance and sensory quality. In this study, a multifunctional chitosan (CS)-based film was fabricated by incorporating a bacterial cellulose/oregano essential oil (BC/OEO) Pickering emulsion, with hydrogen bonding promoting cohesive matrix integration. The film's physicochemical properties, along with its antimicrobial and antioxidant activities, were systematically evaluated.
View Article and Find Full Text PDFCarbohydr Polym
November 2025
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address:
Environmentally friendly food packaging has emerged as a viable strategy to replace traditional plastic films. In this study, eugenol Pickering emulsion was constructed with konjac glucomannan (KGM) and tragacanth gum (GT) as stabilizers, and was introduced into the KGM/chitosan (CS) composite film by electrostatic action to develop a new type of active packaging film. Interfacial characterization revealed optimal emulsion stability at a 1:5 KGM-to-GT mass ratio.
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