Performance of konjac glucomannan/tragacanth gum-stabilized eugenol particles in konjac glucomannan/chitosan films and their application in blueberry preservation.

Environmentally friendly food packaging has emerged as a viable strategy to replace traditional plastic films. In this study, eugenol Pickering emulsion was constructed with konjac glucomannan (KGM) and tragacanth gum (GT) as stabilizers, and was introduced into the KGM/chitosan (CS) composite film by electrostatic action to develop a new type of active packaging film. Interfacial characterization revealed optimal emulsion stability at a 1:5 KGM-to-GT mass ratio.

Polysaccharide-based antifreeze hydrogels in food preservation: From molecular design to smart food packaging applications.

Polysaccharide-based antifreeze hydrogels mark a significant advancement in cold-chain food preservation technology. This hydrogel exhibits excellent water retention and freeze resistance, thereby effectively safeguarding the quality of food in low-temperature environments. This review explores these materials' fundamental principles and practical applications in food science, focusing on their role in smart packaging systems.

Design and applications of antifreeze polysaccharide-based hydrogels for cryoprotection and biotechnological advancements: A review.

Polysaccharide-based hydrogels possess desirable characteristics such as flexibility, biocompatibility, and biodegradability. Nevertheless, these hydrogels frequently lose their inherent traits and functionality under low-temperature circumstances, which significantly restricts their potential applications in cold environments. Antifreeze hydrogels provide a promising solution to this challenge by maintaining their properties at cold temperatures, showcasing remarkable advantages.

Centrosome-assisted assembly of the Balbiani body.

The Balbiani body (Bb), which was discovered about 170 years ago, is a membraneless organelle in the oocyte in most species. In organisms like and Zebrafish, Bb accumulates mitochondria, endoplasmic reticulum (ER), and germline determinants and regulates the proper localization of germline determinants. The Bb forms around the centrosome in the oocyte during early oogenesis.

Dzip1 is dynamically expressed in the vertebrate germline and regulates the development of Xenopus primordial germ cells.

Primordial germ cells (PGCs) are the precursors of sperms and oocytes. Proper development of PGCs is crucial for the survival of the species. In many organisms, factors responsible for PGC development are synthesized during early oogenesis and assembled into the germ plasm.

Dzip1 is dynamically expressed in the vertebrate germline and regulates the development of primordial germ cells.

Primordial germ cells (PGCs) are the precursors of sperms and oocytes. Proper development of PGCs is crucial for the survival of the species. In many organisms, factors responsible for PGC development are synthesized during early oogenesis and assembled into the germ plasm.

Folic acid-maltodextrin polymer coated magnetic graphene oxide as a NIR-responsive nano-drug delivery system for chemo-photothermal synergistic inhibition of tumor cells.

The combination of chemo-photothermal therapy with high efficiency and fewer side effects has a good application prospect in cancer treatment. It is of great significance to construct a nano-drug delivery system with cancer cell targeting, high drug loading and excellent photothermal conversion efficiency. Therefore, a novel nano-drug carrier MGO-MDP-FA was successfully constructed by coating folic acid-grafted maltodextrin polymers (MDP-FA) on the surface of FeO-modified graphene oxide (MGO).

Fancd2os Reduces Testosterone Production by Inhibiting Steroidogenic Enzymes and Promoting Cellular Apoptosis in Murine Testicular Leydig Cells.

Backgruound: It is well-established that serum testosterone in men decreases with age, yet the underlying mechanism of this change remains elusive.

Methods: The expression patterns of Fancd2 opposite-strand (Fancd2os) in BALB/c male mice and testicular tissue derived cell lines (GC-1, GC-2, TM3, and TM4) were assessed using real-time polymerase chain reaction (RT-PCR), Western blot and immunofluorescence. The Fancd2os-overexpressing or knockdown TM3 cells were constructed by infecting them with lentivirus particles and were used to evaluated the function of Fancd2os.

Folic acid and deoxycholic acid derivative modified FeO nanoparticles for efficient pH-dependent drug release and multi-targeting against liver cancer cells.

The novel nano-drug carrier (FDCA-FA-MNPs) was constructed by grafting formyl deoxycholic acid (FDCA) and folic acid (FA) on the surface of FeO magnetic nanoparticles (MNPs), possessing the advantages of superparamagnetism, good stability, low cytotoxicity and good blood compatibility. The hydrophobic anti-cancer drug doxorubicin hydrochloride (DOX) was successfully loaded onto FDCA-FA-MNPs through supramolecular interactions (hydrogen bond between FDCA and drug and hydrophobic interaction and π-π stacking between drug and drug). The drug loading amount and drug loading capacity were 509.

miR-222-3p is involved in neural tube closure by directly targeting Ddit4 in RA induced NTDs mouse model.

Previously our results showed miR-222-3p was significantly downregulated in retinoic acid-induced neural tube defect (NTD) mouse model through transcriptome. Down-regulation of miR-222-3p may be a causative biomarker in NTDs. In this study, RNA was extracted from mouse embryos at E8.

Triformyl cholic acid and folic acid functionalized magnetic graphene oxide nanocomposites: Multiple-targeted dual-modal synergistic chemotherapy/photothermal therapy for liver cancer.

Photo-chemotherapy (PCT) reveals great potential in hepatocellular carcinoma (HCC) treatment, therefore the construct of smart PCT nano-agents with high photothermal conversion efficiency and accurate drug delivery is of great significant. Herein, a novel hybrid nanomaterial MGO-TCA-FA has been designed and constructed by grafting the triformyl cholic acid (TCA) and folic acid (FA) on the surface of FeO modified graphene oxide (MGO). The doxorubicin hydrochloride (DOX) as a model drug could be effectively loaded on the MGO-TCA-FA via hydrogen bonding and π-π stacking (the drug loading amount was 1040 mg/g).

Exosomes from human umbilical cord Mesenchymal stem cells attenuates stress-induced hippocampal dysfunctions.

Human Mesenchymal Stem Cells (MSCs) especially human umbilical cord MSCs is the novel regenerative cell resource for regenerative therapy. However, the biological underpinning of MSCs in neuroprotections requires deep understanding. Exosomes is an important biological factor due to its multiple types of contents with various biological function.

Nkx2.1 downregulation is involved in brain abnormality induced by excess retinoic acid.

Abnormal development of central nervous system (CNS) caused by neural tube defects is not only a major contributor in the prevalence of stillbirths and neonatal deaths but also causes lifelong physical disability in surviving infants. Due to insufficient known investigated causes, CNS developmental abnormality has brought sever burden on health around the world. From previous results of high throughput transcriptome sequencing, we selected transcription factor Nkx2.

Comparative proteomic analysis of round and elongated spermatids during spermiogenesis in mice.

Spermiogenesis in mammals is an exclusive process during which haploid round spermatids mature into spermatozoa in the testis. Any abnormality in the process of spermiogenesis may result in male infertility. The aim of the present study was to characterize the differentially expressed proteins between round and elongated spermatids in mice using label-free quantitative mass spectrometry.

TMPyP4-regulated cell proliferation and apoptosis through the Wnt/β-catenin signaling pathway in SW480 cells.

Background: The aim of this study was to investigate the potential effects of the 5, 10, 15, 20-tetrakis (1-methylpyridinium-4-yl) porphyrin (TMPyP4) on the proliferation and apoptosis of SW480 cells and the underlying mechanisms by which TMPyP4 exerted its actions.

Methods: After treated with different doses of TMPyP4, cell viability was determined by MTT method, the apoptosis was observed by flow cytometry (FCM) and the expression of Wnt, GSK-3β, β-catenin and cyclinD1 was measured by RT-PCR and Western blot analysis.

Results: The analysis revealed that TMPyP4 potently suppressed cell viability and induced the apoptosis of SW480 cells in a dose-dependent manner.

[Preparation and identification of DNA G-quadruplex antibody].

Objective: To construct a prokaryotic expression plasmid of DNA G-quadruplex antibody, express it in E.coli BL21 (DE3) bacterial expression system, purify and identify the antibody.

Methods: Chemically synthesized BG4 gene of DNA G-quadruplex antibodies was inserted into pSANG10 plasmid to construct DNA G-quadruplex antibody expression vector pSANG10-BG4.

Suppression of invasive properties of colorectal carcinoma SW480 cells by 15-hydroxyprostaglandin dehydrogenase gene.

Colorectal carcinoma (CRC) is often lethal when invasion and/or metastasis occur. NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH), an enzyme involved in prostaglandin (including PGE(2)) bio-inactivation, is down-expressed in several epithelial malignancies including CRC. Although its role in the suppression of colon tumorigenesis has been well learned, little is known about the role of 15-PGDH in the process of tumor metastasis.

ECRG2 inhibits cancer cell migration, invasion and metastasis through the down-regulation of uPA/plasmin activity.

The esophageal cancer-related gene 2 (ECRG2) is a novel gene that shows sequence similarity to KAZAL-type serine protease inhibitor. In this study, the migration and invasion of PG cancer cells were inhibited by ectopic expression of ECRG2 in vitro, and metastases decreased after injecting PG/pcDNA3.1-ECRG2 cells into the tail veins of nude mice.

[Inhibitory effects of esophageal cancer related gene 2 on proliferation of human esophageal cancer cell EC9706].

Objective: To investigate the inhibitory role of esophageal cancer related gene 2 (ECRG2) on proliferation of human esophageal cancer cell EC9706.

Methods: Recombinant plasmid pcDNA3.1-ECRG2 with ECRG2 open reading frame was constructed.