Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Low back pain influences people of every age and is one of the major contributors to the global cost of illness. Intervertebral disc degeneration (IVDD) is a major contributor to low back pain, but its pathogenesis is unknown. Recently, ferroptosis has been shown to have a substantial role in modulating IVDD progression. However, the function of ferroptosis-related long non-coding RNAs (lncRNAs) has rarely been reported in IVDD. Consequently, the research was conducted to explore the ferroptosis-related lncRNA signature in the IVDD occurrence and development. We analyzed two datasets (GSE167199 and GSE167931) archived in the NCBI Gene Expression Omnibus (GEO) public database. We screened differentially expressed genes (DEGs) and differentially expressed lncRNAs (DELncs) in these datasets using the limma package. Ferroptosis-related genes (FRGs) were derived from the FerrDb V2 website and the intersection of DEGs and FRGs was considered as differentially expressed ferroptosis-related genes (DFGs). These genes were then subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. Correlations between DFGs and DELncs were shown by Pearson test to determine differential expression of ferroptosis-related lncRNAs. The Pearson test showed that CPEB1-HTR2A-AS1 and ACSL3-DNAJC27-AS1 pairs had correlation coefficients over 0.9. Twenty ferroptosis-related lncRNAs were identified and validated in IVDD. Eight of these lncRNAs were upregulated in IVDD nucleus pulposus cells, including HTR2A-AS1, MIF-AS1, SLC8A1-AS1, LINC00942, DUXAP8, LINC00161, LUCAT1 and LINC01615. Twelve were downregulated in IVDD nucleus pulposus cells, including DNAJC27-AS1, H19, LINC01588, LINC02015, FLNC1, CARMN, PRKG1-AS1, APCDD1L-DT, LINC00839, LINC00536, LINC00710 and LINC01535. Eighteen of the 20 lncRNAs (excluding H19 and LUCAT1) were identified as ferroptosis-related lncRNAs for the first time and verified in IVDD. We have identified a ferroptosis-related lncRNA signature involved in IVDD and revealed a close relationship between CPEB1 and HTR2A-AS1, and between ACSL3 and DNAJC27-AS1. Our findings indicate that ferroptosis-related lncRNAs are a new target set for the early detection and therapy of IVDD.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.gene.2024.148381 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A-to-I edited SNHG3 promotes non-small cell lung cancer metastasis by promoting fatty acid oxidation and resisting ferroptosis.
Commun Biol
September 2025
The Key Laboratory of Advanced Interdisciplinary Studies, School of Public healthy, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
Adenosine-to-inosine (A-to-I) RNA editing is a critical post-transcriptional modification that enhances tumor genome diversity and contributes to cancer progression. In non-small cell lung cancer (NSCLC), while specific A-to-I editing events have been identified, their functional mechanisms and clinical relevance remain poorly understood. Here, through whole-transcriptome analysis of NSCLC specimens, we discovered a hyper-editing event at position c.
View Article and Find Full Text PDFSingle-cell, spatial and bulk transcriptome data analysis revealed LINC00467-mediated Sertoli cell ferroptosis is a potential therapeutic target and biomarker for azoospermia.
Free Radic Biol Med
August 2025
NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China; Clinical Research Center for Reproduction and Genetics in Hunan Province, Reproductive and Genet
Human spermatogenesis is an important physiological process related to programmed cell death. However, which type of programmed cell death playing a key role in normal and abnormal human spermatogenesis remains obscure. This study integrated single-cell, bulk RNA and spatial transcriptome data analysis and found that the ferroptosis signal plays a potential role in spermatogenesis and significantly elevate in testicular samples from humans with non-obstructive azoospermia (NOA) due to various factors.
View Article and Find Full Text PDFPotential novel diagnostic biomarkers of atrial fibrillation: four ferroptosis-related genes linking immune infiltration.
Eur J Med Res
August 2025
Department of Cardiology, The Second Affiliated Hospital, Guangxi Medical University, No.166, Daxue Dong Road, Nanning, 530007, Guangxi, People's Republic of China.
Background: Atrial fibrillation (AF) is a common atrial arrhythmia in clinic, regulated by the immune system and associated with ferroptosis. We hypothesized that combining the analysis of ferroptosis and immune infiltration in AF will help identify more precise diagnostic biomarkers.
Methods: We analyzed two gene expression omnibus (GEO) data sets (GSE41177 and GSE122188) and extracted characteristic ferroptosis-related genes related to sinus rhythm and AF via bioinformatic analysis.
LncRNA HNF1A-AS1 inhibits ferroptosis in oral squamous cell carcinoma cells through the miR-124/ PKM2 axis.
J Stomatol Oral Maxillofac Surg
August 2025
School of Stomatology, Jiangxi Medical College, Nanchang University, Nanchang 330006, China; Jiangxi Provincial Key Laboratory of Oral Diseases, Nanchang 330006, China; Jiangxi Provincial Clinical Research Center for Oral Diseases, Nanchang 330006, China. Electronic address:
Objectives: The aim of this study was to illustrate the molecular mechanism of lncRNA HNF1A-AS1 in ferroptosis in OSCC, providing novel therapeutic implications for OSCC treatment.
Methods: Human OSCC cell lines (CAL-27, SCC-15, HSC-3, WSU-HN12) and normal human oral keratinocytes (NHOK) were used for in vitro experiments. The function of lncRNA HNF1A-AS1 on ferroptosis in OSCC was evaluated through measurement of cell proliferation, gene expression, protein expression levels, and ferroptosis-related indicators.
A ferroptosis-related risk model for non-coding RNA AC002331.1 in colon cancer: construction via competing endogenous RNA network analysis.
Discov Oncol
August 2025
Department of Clinical Research Center, Central Hospital, Shandong First Medical University, No. 105, Jiefang Road, Lixia District, Jinan City, 250013, Shandong Province, China.
Background: Colon cancer, a globally prevalent malignancy with high mortality, involves lncRNA regulation, ferroptosis pathway abnormalities, and gut microbiota dysbiosis. Ferroptosis-related gene models may aid prognostic evaluation, while microbiota metabolites modulate ferroptosis in contexts like ulcerative colitis.
Methods: Using the GEO dataset (GSE97300), we screened differentially expressed lncRNAs (e.