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http://dx.doi.org/10.1002/ctm2.1454 | DOI Listing |
J Genet
July 2025
College of Agronomy, Center for Crop Genome Engineering, Henan Agricultural University, Zhengzhou 450046, People's Republic of China,
Heterotrimeric G-proteins are multifunctional modulators that participate in a wide range of growth and developmental processes in eukaryotic species, including yeast, plants, and animals. In this study, we characterized a maize mutant, , that showed a compact architecture and reproductive-organ-related phenotypic variation. Heredity analysis indicated that the mutant phenotypes resulted from monogenic inheritance.
View Article and Find Full Text PDFInfect Med (Beijing)
June 2025
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Backgroud: Coltiviruses are spherical, non-enveloped viruses with 12 double-stranded RNA segments, belonging to the family , and predominantly transmitted by ticks. This study isolated and characterized a novel coltivirus, designated Woodland tick reovirus (WLTRV), from ticks collected in Helong City, Jilin Province, in Northeastern China.
Methods: SW-13 and Vero cells were used to isolate WLTRV through three blind passages, while seven mammalian cell lines assessed viral growth.
Nucleic Acids Res
March 2024
Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Center for Infection Research (HZI), Würzburg, Germany.
RNA-protein interactions determine the cellular fate of RNA and are central to regulating gene expression outcomes in health and disease. To date, no method exists that is able to identify proteins that interact with specific regions within endogenous RNAs in live cells. Here, we develop SHIFTR (Selective RNase H-mediated interactome framing for target RNA regions), an efficient and scalable approach to identify proteins bound to selected regions within endogenous RNAs using mass spectrometry.
View Article and Find Full Text PDFACS Nano
December 2023
Institute of Molecular Medicine (IMM), Renji Hospital, Shanghai Jiao Tong University School of Medicine, and College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Aptamers are single-stranded DNA or RNA molecules capable of recognizing targets via specific three-dimensional structures. Taking advantage of this unique targeting function, aptamers have been extensively applied to bioanalysis and disease theranostics. However, the targeting functionality of aptamers in the physiological milieu is greatly impeded compared with their applications.
View Article and Find Full Text PDFViruses
October 2023
Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City 21934, Egypt.
Despite its first description in 1977 and numerous reports of its presence in various plant species in many countries, the molecular information available in GenBank for artichoke Italian latent virus (AILV) is still limited to a single complete genome sequence (RNA1 and 2) of a grapevine isolate (AILV-V) and a partial portion of the RNA2 sequence from an isolate of unknown origin and host. Here, we report the results of molecular analyses conducted on the RNA2 of some AILV isolates, sequenced for the first time in this study, together with the first-time identification of AILV in a new host plant species, namely chard ( subsp. vulgaris), associated with vein clearing and mottling symptoms on leaves.
View Article and Find Full Text PDF