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http://dx.doi.org/10.1097/JS9.0000000000000378 | DOI Listing |
J Biomed Res
September 2025
State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University; Nanjing, Jiangsu 211166, China.
Non-obstructive azoospermia (NOA), characterized by impaired spermatogenesis and the complete absence of sperm in the ejaculate, represents one of the most severe forms of male infertility. Current diagnostic strategies rely on invasive procedures such as testicular sperm extraction, underscoring the urgent need for reliable, non-invasive alternatives. In the present study, we performed untargeted metabolomic profiling of human seminal plasma to identify biomarker panels capable of stratifying azoospermia subtypes through a stepwise approach.
View Article and Find Full Text PDFNanoscale Horiz
September 2025
Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Extracellular vesicles (EVs) have emerged as valuable sources for liquid biopsy in disease diagnostics, given their protein and nucleic acid cargoes (, miRNA, mRNA, glycoRNA) can serve as critical biomarkers. DNA nanotechnology, leveraging its inherent programmability, high specificity, and powerful signal amplification capability, offers a transformative approach for the bioanalysis of EVs. This review summarizes recent advances in DNA nanotechnology-based analytical methodologies for detecting EV-associated proteins and nucleic acids.
View Article and Find Full Text PDFCancer Biol Med
September 2025
Department of Oncology, Hunan Provincial People's Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 410013, China.
Objective: Osimertinib (OSI) therapy, a cornerstone in treating non-small cell lung cancer (NSCLC), has been severely limited by rapidly developing acquired resistance. Inhibition of bypass activation using a combination strategy holds promise in overcoming this resistance. Biguanides, with excellent anti-tumor effects, have recently attracted much attention for this potential.
View Article and Find Full Text PDFJ Am Chem Soc
September 2025
Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
Non-hydrogenative para-hydrogen-induced polarization (nhPHIP) has proven a powerful tool for the enhanced NMR detection of several classes of metabolites in complex mixtures. Particularly, compounds carrying an α-amino acid motif have been previously detected and quantified in biological samples and natural extracts at submicromolar concentrations using 2D nhPHIP NMR spectroscopy. This technique is here applied for the first time in a semi-targeted metabolomics NMR study on urine from patients suffering from Pyridoxine-Dependent Epilepsy (PDE), currently diagnosed by the presence of dilute unique biomarkers.
View Article and Find Full Text PDFNeuropsychopharmacol Rep
September 2025
Trivedi Science Research Laboratory Pvt. Ltd., Thane (W), Maharashtra, India.
Reduced physical activities, social support, old age, and increased exposure to regular stressful life events are crucial factors for poor mental health. Although several medicines are available to support mental health-related symptoms, due to more side effects and non-compliance for long-term use, these conventional medicines are not appropriate to tackle these symptoms. In this context, the study aimed to determine whether spiritual energy therapy with an experienced spiritual energy practitioner could manage adults' psychological and mental health-related symptoms.
View Article and Find Full Text PDF