Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The role of peptidoglycan-associated lipoprotein (Pal) in A. baumannii pathogenesis remains unclear. Here, we illustrated its role by constructing a pal deficient A. baumannii mutant and its complementary strain.Transcriptome analysis of the WT and pal mutant revealed a total of 596 differentially expressed genes. Gene Ontology analysis revealed that pal deficiency caused the downregulation of genes related to material transport and metabolic processes. The pal mutant showed a slower growth and was sensitive to detergent and serum killing compared to WT strain, whereas, the complemented pal mutant showed rescued phenotype. The pal mutant caused decreased mortality in mice pneumonia infection compared to WT strain, while the complemented pal mutant showed increased mortality. Mice immunized with recombinant Pal showed 40% protection against A. baumannii-mediated pneumonia. Collectively, these data indicate Pal is a virulence factor of A. baumannii and may serve as a potential target for preventive or therapeutic interventions.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ygeno.2023.110590 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nuclear hormone receptor regulation of PAL-1/Caudal mediates ventral nerve cord assembly in C. elegans.
Dev Biol
September 2025
Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Canada. Electronic address:
The regulatory network governed by CDX/Caudal family transcription factors plays critical roles in shaping embryonic neural development. In C. elegans, we found that proper expression of pal-1, the C.
View Article and Find Full Text PDFUse of p53 immunohistochemistry can improve diagnostic agreement for differentiated vulvar intraepithelial neoplasia (dVIN): an international reproducibility study.
Histopathology
August 2025
Department of Pathology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Aims: Differentiated or HPV-independent vulvar intraepithelial neoplasia (dVIN) can progress rapidly to invasive cancer and accurate pathological diagnosis is essential to facilitate appropriate interventions. Histological similarities of dVIN with non-neoplastic lesions, however, often make the diagnosis less reproducible. We investigated among a diverse group of pathologists whether the diagnostic agreement improves with the use of p53 immunohistochemistry (IHC) interpreted using the pattern-based schema.
View Article and Find Full Text PDFMotifs in the histone H3 and H4 and chromatin modifying factors are crucial to regulate the multifactorial zinc response pathway in Saccharomyces cerevisiae.
Arch Biochem Biophys
October 2025
Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal, India. Electronic address:
Zinc is a trace element that plays a crucial role in regulating various biological processes. To identify the factors involved in maintaining zinc homeostasis, we analysed the growth of histone mutants and gene deletions of chromatin-modifying factors. Growth assays revealed that specific regions and amino acid residues of histones H3 and H4, along with several chromatin-modifying factors, are essential for survival under zinc stress.
View Article and Find Full Text PDFMutagenesis of Highland barley (Hordeum vulgare L. Var. nudum) using nitrogen ion beam implantation: screening of phenotypic Var.ations and comparative transcriptome analysis.
BMC Genomics
July 2025
Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu, 610064, P. R. China.
Background: Highland barley (Hordeum vulgare L. var. nudum) is a key crop of the Qinghai-Tibet Plateau, renowned for its nutritional value and exceptional adaptability to high-altitude environments.
View Article and Find Full Text PDFImmunization with glutamine synthetase-deficient live attenuated parasites induces the differentiation of memory T cells to protect hosts from virulent infection of Leishmania donovani.
Vaccine
July 2025
Cellular Immunology and Vector Molecular Biology Laboratory, Department of Zoology, West Bengal State University, Barasat, North 24 Parganas, West Bengal, India. Electronic address:
Visceral leishmaniasis (VL) is a major threat to economically challenged sectors of the world, and there are only limited options in vaccines available at present. Vaccination using live attenuated strains is preferred for their retention of true antigens, however, the major concern about these vaccines is raised by their ability to protect from virulent challenge at the early time point and also bearing the risk of a potential outbreak of their own. In this study, we reported a metabolically deficient glutamine synthetase double knock-out live attenuated strain of Leishmania donovani (LdGS) that, with a very low inoculum single dose, is highly effective in generating an IFN-γ Th1-mediated immunity during virulent challenge as early as 2 weeks post-challenge time point.
View Article and Find Full Text PDF