A nanofiber-hydrogel composite from green synthesized AgNPs embedded to PEBAX/PVA hydrogel and PA/Pistacia atlantica gum nanofiber for wound dressing.

A polyamide/Pistacia atlantica (P.a) gum nanofiber, fabricated by electrospinning method, was coated on a layer of PEBAX/PVA hydrogel embedded with green synthesized Ag nanoparticles (AgNPs) and the prepared nanofiber-hydrogel composite was assessed for wound dressing application. The AgNPs were characterized using ultraviolet-visible (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zeta potential analysis. The PEBAX/PVA/Ag hydrogel, prepared using solution casting method, displayed strong mechanical properties as Young's modulus and the elongation at break for the hydrogel containing AgNPs increased by 12 % and 96 %, respectively. The PEBAX/PVA/Ag hydrogel showed a high antimicrobial activity towards the E. coli (22.8 mm) with no cytotoxicity. The effect of adding the P.a gum on the properties of polyamide nanofiber was investigated using FTIR, SEM, and tensile tests. Samples were assessed by swelling, degradation, and water vapor transfer measurements. Very fine and continuous fibers with average diameters of ≤200 nm were observed by SEM analysis due to the addition of the P.a gum. The result of tensile test indicated that the addition of P.a gum improves the mechanical properties of nanofibers. The physical properties and biocompatibility of the two layers were shown to be complementary when combined.