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Methods to synthesize influenza virus inhibitors with fluoro, phosphono, and/or sulfo functional groups are described. The resulting sialic acid analogues are produced from the natural substrate N-acetylneuraminic acid as starting material. Fluorescent assay methods for inhibition of influenza neuraminidase and virus proliferation are also provided.
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http://dx.doi.org/10.1007/978-1-0716-2635-1_20 | DOI Listing |
Phytomedicine
August 2025
School of Chinese Materia Medica, Beijing University of Chinese Medicine, 102488, China. Electronic address:
Background: Diabetic nephropathy (DN), a serious diabetic complication, currently has limited treatment options. Yulan Jiangtang capsules (YL) are a clinically approved traditional Chinese medicine formula for glycemic control and diabetes-related complications. Nevertheless, the underlying mechanisms of their therapeutic effects remain incompletely elucidated.
View Article and Find Full Text PDFJ Hum Genet
September 2025
Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan.
GNE myopathy is an autosomal recessive distal myopathy resulting from biallelic pathogenic variants in the GNE gene, a key enzyme in sialic acid biosynthesis. Although most pathogenic variants are missense variants, recent advances have enabled the identification of copy number variations, deep intronic variants, and regulatory changes in the promoter region, significantly enhancing diagnostic accuracy. Progress in genetic diagnostics now allows detection of rare and complex variants.
View Article and Find Full Text PDFPLoS Pathog
September 2025
State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Grass carp reovirus (GCRV) causes hemorrhagic disease and substantial economic losses in the aquaculture of grass carp (Ctenopharyngodon idella), a commercially important fish species in China. Although viral entry depends on interactions between viral proteins and host receptors, the specific host molecules mediating this process have not been fully elucidated. Here, we identify cell surface sialic acid (SA) as a critical functional receptor for GCRV.
View Article and Find Full Text PDFMol Hum Reprod
September 2025
Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, India.
Human fertilization is a coordinated process involving nteraction of sperm with the oocyte. As the sperm pass through the female reproductive tract (FRT), they are presented with numerous challenges. These include navigating through highly viscous cervical mucus while evading immune responses to successfully fertilize the oocyte.
View Article and Find Full Text PDFBioelectrochemistry
September 2025
Division of Analytical Chemistry, Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan. Electronic address:
Early and accurate detection of circulating tumor cells (CTCs) is vital for cancer diagnosis and personalized treatment. Despite their clinical significance, the identification of CTCs remains challenging because of the biological complexity and lower concentration. Therefore, a cost-effective, and label-free electrochemical biosensor based on phenyl boronic acid functionalized graphene oxide-silica (PBA@GO-SiO) is developed to selectively recognize sialic acid-rich glycoproteins on HepG2 cells.
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