Fabrication of phenylboronic acid modified graphene oxide-silica biosensor for label-free electrochemical detection of circulating tumor cells.

Early and accurate detection of circulating tumor cells (CTCs) is vital for cancer diagnosis and personalized treatment. Despite their clinical significance, the identification of CTCs remains challenging because of the biological complexity and lower concentration. Therefore, a cost-effective, and label-free electrochemical biosensor based on phenyl boronic acid functionalized graphene oxide-silica (PBA@GO-SiO) is developed to selectively recognize sialic acid-rich glycoproteins on HepG2 cells.

Human serum N-glycome profiling via the newly developed asparagine immobilized cellulose/polymer nanohybrid.

Human serum N-linked glycans expression levels change during the disease progression. The low abundance, structural diversity, and coexisting matrices hinder their detection in mass spectrometry analysis. Considering the hydrophilic nature of N-glycans, cellulose/polymer (1,2-Epoxy-5-hexene) nanohybrid is fabricated with oxirane groups functionalized of asparagine to develop solid phase extraction based hydrophilic interaction liquid chromatography sorbent (cellulose/1,2-Epoxy-5-hexene/asparagine).