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Article Abstract
Sugarcane is of important economic value for producing sugar and bioethanol. (old name: ) is an intergeneric wild species of sugarcane that has desirable resistance traits for improving sugarcane varieties. However, the scarcity of chromosome markers has hindered the cytogenetic study of . Here we applied maize chromosome painting probes (MCPs) to identify chromosomes in sorghum and using a repeated fluorescence in situ hybridization (FISH) system. Sequential FISH revealed that these MCPs can be used as reliable chromosome markers for , even though has diverged from maize over 18 MYs (million years). Using these MCPs, we identified chromosomes based on their sequence similarity compared to sorghum and labeled them 1 through 10. Then, the karyotype of was established by multiple oligo-FISH. Furthermore, FISH results revealed that 5S rDNA and 35S rDNA are localized on chromosomes 5 and 6, respectively, in . Altogether, these results represent an essential step for further cytogenetic research of in sugarcane breeding.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8395171 | PMC |
| http://dx.doi.org/10.3390/ijms22168539 | DOI Listing |
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