Identification of novel sources and genetic mapping for Bacterial Leaf Streak resistance in a geographically diverse panel of wheat.

Bacterial leaf streak (BLS), caused by pv. (), has recently emerged as a significant threat to wheat production in the Northern Great Plains region of the US. Deploying resistant cultivars is an economical and practical method of controlling BLS.

Lymphoma driver mutations at the root of somatic evolution of nerve-damaging autoantibodies in myelin associated glycoprotein neuropathy.

Background: In autoimmune disease it is not understood how self-reactive B cells escape immune tolerance checkpoints to produce pathogenic autoantibodies.

Objective: In patients with demyelinating polyneuropathy caused by IgM autoantibodies against myelin associated glycoprotein (MAG) and the sulphated trisaccharide CD57, we aimed to test the hypothesis that B cells making the autoantibody escaped tolerance by acquiring lymphoma driver somatic mutations.

Methods: Deep single-cell RNA, DNA, flow cytometric and antibody specificity analysis of blood from three patients with MAG neuropathy.

Point-of-care ultrasound of the upper airway in difficult airway management: a systematic review and meta-analysis.

Introduction: The utility of bedside screening tests for the prediction of difficult airways is limited. There is growing interest in the role of point-of-care-ultrasound in airway assessment and management. This systematic review and meta-analysis aimed to determine the diagnostic utility and clinical application of various upper airway point-of-care-ultrasound parameters in the prediction of difficult airways.

Evaluating the Effect of a Sleep Prehabilitation Intervention in Patients Awaiting Elective Surgery: Protocol for a Single-Blind Randomised Trial.

Adequate sleep health is critical for surgical recovery. Disrupted sleep can impede wound healing and cognitive performance and contribute to poor surgical outcomes. Preoperative intervention aimed at improving surgical outcomes is often referred to as prehabilitation and commonly uses exercise, nutrition or psychological intervention.

Improving Patient Sleep Before Surgery: The Case for Sleep Prehabilitation.

Sleep health includes quality, quantity, and timing of sleep and is negatively affected by surgery. Poor preoperative sleep health is associated with worse surgical outcomes, including greater morbidity and hospital length of stay. In this narrative review, we describe the rationale and supporting evidence for preoperative sleep interventions, or sleep prehabilitation, to improve perioperative outcomes.

TEMPORARY REMOVAL: Causes of Myocardial Infarction in Younger Patients: Troponin-Elevation in Persons ≤65 Years Old in Olmsted County.

The publisher regrets that this article has been temporarily removed. A replacement will appear as soon as possible in which the reason for the removal of the article will be specified, or the article will be reinstated. The full Elsevier Policy on Article Withdrawal can be found at https://www.

Spirometry update 2019: phase 4 forced maximal inspiration and new acceptability criteria.

https://bit.ly/44Cqz6v.

Procedural and Clinical Outcomes After Orbital Atherectomy Versus Intravascular Lithotripsy in Patients With Calcified Nodules.

Background: Calcified nodules (CNs) remain a major challenge in percutaneous coronary intervention (PCI). We sought to compare procedural and clinical outcomes after orbital atherectomy (OA) versus intravascular lithotripsy (IVL)-facilitated PCI in patients with CNs.

Methods: We identified patients with optical coherence tomography (OCT)-defined CNs who underwent PCI with either OA or IVL between 2012 and 2022 and had both pre- and post-PCI OCT images available.

The Effect of Sleep Disorder Interventions in Patients With Obstructive Sleep Apnea Undergoing Solid-Organ Transplants: A Systematic Review.

Poor sleep results in a wide range of health consequences, and is prevalent among patients undergoing solid-organ transplant, who have high rates of obstructive sleep apnea (OSA). Our objective was to synthesize the evidence examining the impact of OSA therapies such as continuous positive airway pressure (CPAP) on outcomes in the solid-organ transplant setting. We conducted a systematic review registered via PROSERO (CRD42024512577) and reported in accordance with PRISMA guidelines.

Assessing the role of drug interventions in the polycystic ovary syndrome model.

The research was devised to assess the prophylactic effects of agomelatine, a melatonin receptor antagonist, against letrozole-induced polycystic ovarian syndrome (PCOS) in 56 female rats divided randomly into seven groups of eight rats each. Group I served as the control and was exposed to behavioral tests such as the elevated plus maze (EPM) and Morri's water maze (MWM). Group II received 10 ml/kg of 1% carboxymethyl cellulose (CMC) orally for 28 days as a vehicle control.

Visualizing Relaxation in Wearables: Multi-Domain Feature Fusion of HRV Using Fuzzy Recurrence Plots.

Traditional relaxation techniques such as meditation and slow breathing often rely on subjective self-assessment, making it difficult to objectively monitor physiological changes. Electrocardiograms (ECG), which are commonly used by clinicians, provide one-dimensional signals to interpret cardiovascular activity. In this study, we introduce a visual interpretation framework that transforms heart rate variability (HRV) time series into fuzzy recurrence plots (FRPs).

RNA extraction-free CRISPR-based SARS-CoV-2 detection in viral transport medium and dry swab-a comparative analysis: Short title: Extraction-free CRISPR-based Covid detection.

CRISPR-Cas-based methods have shown high efficacy in detecting SARS-CoV-2. Amidst the Covid-19 pandemic, numerous studies have explored SARS-CoV-2 detection methods without the need for RNA extraction, aiming to reduce cost and processing time. Here, we assessed a CRISPR-based SARS-CoV-2 detection method's ability to detect the virus in viral transport medium (VTM).

Aspirin, Birthweight, and Large-For-Gestational-Age Neonates: A Secondary Analysis of the ASPRE Trial.

Objective: To investigate the effects of aspirin on the distribution of birthweight and its impact on the rates of large-for-gestational-age (LGA) neonates.

Design: Secondary analysis of the Combined Multimarker Screening and Randomised Patient Treatment with Aspirin for Evidence-based Preeclampsia Prevention (ASPRE) trial.

Setting: Thirteen hospitals in England, Spain, Belgium, Greece, Italy and Israel.

The Impact of Chronic Exercise on Cognitive Function: An Overview of Reviews.

Understanding the associations between cognitive function and chronic exercise has gained significant attention in the last decade. This overview aims to consolidate current knowledge and shed light on potential benefits of long-term exercise on cognitive abilities. Relevant databases, including Scopus, PubMed, and Web of Science, were searched through predefined keywords related to chronic exercise and cognitive function.

Design and Validation of Low-Cost, Portable Impedance Analyzer System for Biopotential Electrode Evaluation and Skin/Electrode Impedance Measurement.

This paper presents a novel, low-cost, portable impedance analyzer system designed for biopotential electrode evaluation and skin/electrode impedance measurement, critical for enhancing bioelectrical signal quality in healthcare applications. In contrast with conventional systems that depend on external PCs or host devices for data acquisition, visualization, and analysis, this design integrates all functionalities into a single, compact platform powered by the Analog Devices AD5933 impedance converter and a Raspberry Pi 4. The design incorporates custom analog circuitry to extend the measurement range from 10 Hz to 100 kHz and supports a wide impedance spectrum through switchable feedback resistors.

Automated Real-Time Percutaneous Coronary Intervention Risk Model Leveraging Electronic Health Records.

Background: Automated individualized risk prediction tools linked to electronic health records (EHRs) are not available for patients undergoing percutaneous coronary interventions (PCIs).

Objectives: Our goal was to automatically extract data elements used in the Mayo Clinic PCI models from EHR to enable point of care risk assessment.

Methods: Using the Mayo Clinic PCI registry, variables in the Mayo Clinic PCI risk score were trained and tested in an EHR to identify in-hospital death, stroke, bleeding, acute kidney injury (AKI) in patients who underwent PCI from 2016 to 2024.

Sleep, Health Care-Seeking Behaviors, and Perceptions Associated With the Use of Sleep Wearables in Canada: Results From a Nationally Representative Survey.

Background: The popularity of sleep-tracking wearables has surged worldwide. Yet, there are significant gaps in understanding the real-life implications of this phenomenon. While wearables may offer insights about sleep and promote sleep health awareness, evidence remains mixed on whether they lead to improved sleep outcomes or fuel sleep anxiety.

Impact of Renal Function and Acute Kidney Injury on Long-term Outcomes After Percutaneous Coronary Intervention.

Objective: To determine the incidence, predictors, and prognostic implications of acute kidney injury (AKI) after percutaneous coronary intervention (PCI).

Patients And Methods: Retrospective analysis of the Mayo Clinic PCI registry identified 9199 patients who underwent PCI from January 1, 2009, through June 30, 2023.

Results: A total of 856 patients (9.

A Retrospective Study of Ultramassive Transfusion in Trauma Patients: Is There a Value After Which Additional Transfusions Are Futile?

Background: Hemorrhage is a leading cause of preventable mortality in trauma. During times of blood shortages, it may be prudent to consider a transfusion threshold during massive transfusion after which additional transfusions are futile due to nonsurvivability. The main objective of this study is to examine outcomes associated with ultramassive transfusion (UMT; defined as ≥20 units of red blood cells [RBC] within 24 hours) and determine if there is a threshold beyond which additional transfusion efforts should cease.

Evaluating the role of bromelain in diabetes management: a systematic review of research evidence and mechanisms of action.

Background: The use of complementary and alternative medicine is becoming increasingly important in the prevention and management of complications related to diabetes.

Methods: A comprehensive literature search was conducted across major databases (EMBASE, Scopus, PubMed, Google Scholar, Web of Science, Cochrane Library) up to December 2024. The search focused on studies examining the effect of bromelain on glycemic control, lipid profiles, and related mediators in diabetes.

iPSC-RPE patch restores photoreceptors and regenerates choriocapillaris in a pig retinal degeneration model.

Dry age-related macular degeneration (AMD) is a leading cause of untreatable vision loss. In advanced cases, retinal pigment epithelium (RPE) cell loss occurs alongside photoreceptor and choriocapillaris degeneration. We hypothesized that an RPE-patch would mitigate photoreceptor and choriocapillaris degeneration to restore vision.

Expanded T cell clones with lymphoma driver somatic mutations accumulate in refractory celiac disease.

Intestinal inflammation continues in a subset of patients with celiac disease despite a gluten-free diet. Here, by applying multi-omic single-cell analysis to duodenal biopsies, we found that low-grade malignancies with lymphoma driver mutations in patients with refractory celiac disease type 2 (RCD2) are comprised by surface CD3-negative (sCD3) lymphocytes stalled at an innate lymphoid cell (ILC)-progenitor T cell stage undergoing extensive , , and TCR recombination. In people with refractory celiac disease type 1 (RCD1), a disease currently lacking explanation, we identified sCD3 T cells with lymphoma driver mutations in 6 of 10 individuals with RCD1 and in one of the patients with active, recently diagnosed celiac disease.

Natural History of Microperimetry and Optical Coherence Tomography in USH2A-Retinopathy: A Structure-Function Association Study.

Purpose: To describe the natural history of optical coherence tomography (OCT) and mesopic microperimetry (MP) parameters in the Rate of Progression of USH2A-related Retinal Degeneration (RUSH2A) study.

Design: Multicenter, international, prospective, longitudinal natural history study.

Methods: Study Population: 105 participants with biallelic disease-causing variants in USH2A with a clinical diagnosis of Usher Syndrome or autosomal recessive retinitis pigmentosa who underwent annual testing over 4 years.

Pretreatment With P2Y Inhibitors in Contemporary Practice: Where Do We Stand?

The management of antiplatelet strategies among patients presenting with acute coronary syndrome (ACS) continues to evolve. Earlier studies have shown ischemic benefit with P2Y pretreatment in the setting of ACS; however, large-scale contemporary randomized trials supporting this strategy are lacking. This issue assumes relevance among patients with high bleeding risk or those referred for urgent/emergent coronary artery bypass surgery following coronary angiography.