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http://dx.doi.org/10.1161/ATVBAHA.121.314689 | DOI Listing |
Cancer Sci
September 2025
Department of Molecular Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan.
Histone H3K4 modifications are altered in the regulation of gene expression and in multiple cellular processes during cancer development and progression. Understanding the roles of H3K4-modifying enzymes will provide novel insights into therapeutic tools for cancer treatment. H3K4-modifying enzymes catalyze the addition or removal of covalent modifications with specific substrate preferences.
View Article and Find Full Text PDFMol Ther Nucleic Acids
September 2025
Department of Genetics and Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
Epigenetic modulation enables precise gene regulation without altering DNA sequences. While histone acetylation has been widely utilized for gene activation, the therapeutic potential of histone methylation remains underexplored. In this study, we developed a new epigenetic activator by fusing the histone methyltransferase SETD7 to deactivated Cas9 (dCas9).
View Article and Find Full Text PDFJ Biol Chem
August 2025
Pharmaceutical analysis Laboratory, College of Pharmacy, University of Manitoba, 750 McDermot Avenue West, Winnipeg, Manitoba, R3E 0T5, Canada; Paul Albrechtsen Research Institute, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada. Electronic address:
SET7/9 (SETD7) is a SET domain protein lysine methyltransferase (PKMT). We characterized its activity using a mass spectrometry (MS) assay showing that it follows an ordered sequential enzyme kinetic mechanism where SAM is the first substrate to bind followed by histone H3, and mono-methylated histone H3 is the first product to dissociate, followed by SAH. Full-length histones H2A, H2B and H4 are also substrates for SET7/9.
View Article and Find Full Text PDFInt J Mol Sci
August 2025
Department of Biology, Indiana State University, Terre Haute, IN 47809, USA.
The Complex Proteins Associated with Set1 (COMPASS)-like complex regulates developmental gene expression via histone 3 lysine 4 (H3K4) methylation and other transcriptional mechanisms. Several members of the lysine methyltransferase 2C and D (KMT2C/D)-COMPASS-like complex are implicated in human congenital heart and vascular defects. The investigation of the orthologous Trithorax-related (Trr)-COMPASS-like complex in (the fruit fly) offers a versatile model to explore gene function in the developing heart.
View Article and Find Full Text PDFPlant Cell Environ
August 2025
College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin, China.
Rhizoctonia solani is a soil-borne pathogen causing destructive diseases on various economically important crops. Beneficial plant and fungus interactions are well acknowledged to enhance plant resistance to biotic stresses. However, the underlying mechanisms are not fully understood, especially the contribution of fungal epigenetic regulation remains poorly understood.
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