SET7/9 exhibits sigmoidal kinetics on nucleosomes, hyperbolic kinetics on histones by an ordered sequential mechanism and methylates lysine and arginine.

SET7/9 (SETD7) is a SET domain protein lysine methyltransferase (PKMT). We characterized its activity using a mass spectrometry (MS) assay showing that it follows an ordered sequential enzyme kinetic mechanism where SAM is the first substrate to bind followed by histone H3, and mono-methylated histone H3 is the first product to dissociate, followed by SAH. Full-length histones H2A, H2B and H4 are also substrates for SET7/9.

Electrographic Features of Spontaneous Recurrent Seizures in a Mouse Model of Extended Hippocampal Kindling.

Epilepsy is a chronic neurological disorder characterized by spontaneous recurrent seizures (SRS) and comorbidities. Kindling through repetitive brief stimulation of a limbic structure is a commonly used model of temporal lobe epilepsy. Particularly, extended kindling over a period up to a few months can induce SRS, which may simulate slowly evolving epileptogenesis of temporal lobe epilepsy.