Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/10428194.2021.1894643 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection of Microorganisms Causing Human Respiratory Infection Using One-Tube Multiplex PCR.
Infect Dis Rep
August 2025
Instituto de Biotecnologia, Universidade Federal de Uberlândia, Uberlândia 38405-302, Minas Gerais, Brazil.
Due to the significant overlap in symptoms between COVID-19 and other respiratory infections, a multiplex PCR-based platform was developed to simultaneously detect 22 respiratory pathogens. Target sequences were retrieved from the GenBank database and aligned using Clustal Omega 2.1 to identify conserved regions prioritized for primer design.
View Article and Find Full Text PDFA multiplex allele specific PCR capillary electrophoresis (mASPCR-CE) assay for simultaneously analysis of / copy number and loss-of-function variants.
Clin Chem Lab Med
August 2025
Department of Medical Genetics, School of Basic Medical Sciences, 70570 Southern Medical University, Guangzhou, China.
Objectives: Spinal muscular atrophy (SMA) is a severe inherited neuromuscular disorder with a high carrier frequency and incidence rate. An accurate molecular method for genes is crucial in carrier screening, clinical diagnosis, outcome assessment and precision therapies.
Methods: Comprehensively using the multiplex allele specific PCR (mASPCR) and capillary electrophoresis (CE), a novel single tube assay was developed to simultaneously determine the copy number of // genes and five common loss-of-function variants in .
Simultaneous detection of five shrimp pathogens using a single-tube EvaGreen real-time PCR assay with differential melting temperature.
Appl Environ Microbiol
August 2025
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Key Laboratory of Maricultural Org
The World Organisation for Animal Health (WOAH) has assessed crustacean diseases, such as infections with white spot syndrome virus (WSSV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), decapod iridescent virus 1 (DIV1), and acute hepatopancreatic necrosis disease (AHPND), as listed diseases, and infection with (EHP) as an emerging disease, all of which significantly threaten the shrimp industry. This study developed a quintuplex EvaGreen-based melting curve real-time PCR method for the simultaneous detection of WSSV, IHHNV, DIV1, AHPND-causing (), and EHP. In the specific assay, only the target pathogen demonstrated efficient and detectable amplification, thereby indicating that the method exhibits high specificity.
View Article and Find Full Text PDFDevelopment and validation of a multiplex assay system for simultaneous detection of HIV-1, HBV, and human STRs for forensic application on CE system.
Int J Legal Med
July 2025
Department of Forensic Medicine, School of Basic Medical Sciences, Central South University, No172. Tongzipo Road, Changsha, 410013, Hunan Province, China.
Human immunodeficiency virus (hereafter, "HIV") and hepatitis B virus (hereafter, "HBV") are prevalent infectious pathogens. In many countries, criminal law applies to those who intentionally transmit or expose others to these two dangerous pathogens. Viral infection is a significant characteristic in forensic investigations, and there are few reports on the simultaneous detection of DNA viruses, RNA viruses, and human short tandem repeats (hereafter, "STRs") in forensic laboratories.
View Article and Find Full Text PDFHigh sensitivity and specificity analysis of multiple miRNAs.
Anal Chim Acta
October 2025
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, No 163, Xianlin Avenue, Nanjing, 210023, PR China. Electronic address:
Background: The aberrant expression of microRNAs (miRNAs) is closely associated with the onset and progression of many diseases, particularly in malignant tumors, where it usually involves coordinated changes in the expression levels of multiple miRNAs. However, existing miRNA detection techniques still face many technical challenges in achieving multiplex detection within a single tube. Therefore, there remains a critical need to develop multiplex miRNA detection methods with high specificity, simplicity, and sensitivity.
View Article and Find Full Text PDF