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This study analysed the effect of prior high-pressure processing (HPP; 200-600 MPa, 2 min), freezing (-30 °C, 48 h), and frozen storage (-18 °C, 6 months) on the macroelement and trace element content in brine-canned mackerel (). Most elements (, , , , , , , , , and ) showed an increased ( < 0.05) presence in mackerel muscle canned after freezing. A content increase ( < 0.05) was also observed for and if prior frozen storage was also applied; on the contrary, , , , , , , and showed a content decrease ( < 0.05) as a result of such storage. Freezing, frozen storage, and canning led to lower values ( < 0.05) in canned fish for , , , and . Prior HPP led to relevant content decreases ( < 0.05) for , , , , , , , and contents in fish canned after the freezing step; HPP provoked additional decreases ( < 0.05) in , , and levels in samples corresponding to 6-month frozen storage. On the contrary, prior HPP led to marked increases ( < 0.05) for , , and contents in all canned samples. Content changes are explained on the basis of modifications of other constituents and liquor losses from muscle.
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http://dx.doi.org/10.3390/foods9121868 | DOI Listing |
Ann Lab Med
September 2025
Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea.
Cryopreserved umbilical cord blood (CB) for transplantation is occasionally exposed to room temperature during storage in cord blood banks. We evaluated the effect of room temperature exposure on the quality of cryopreserved CB. Forty frozen CB units stored in liquid nitrogen tanks were exposed to room temperature until they reached a target temperature of -130°C (group I), -60°C (group II), -40°C (group III), or -25°C (group IV) (N=10 in each group) and then re-stored.
View Article and Find Full Text PDFFood Chem
September 2025
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, China; National Engineering Rese
Investigating how cereal-derived polysaccharides mitigate the cold denaturation of dough carries substantial significance for improving the quality of cereal-based products. This study aimed to assess the effects of laboratory-prepared oat β-glucan and commercial oat β-glucan on the structural properties and quality of wheat flour, frozen dough, and the resultant steamed bread. Our data indicated that β-glucan with higher molecular weight (1.
View Article and Find Full Text PDFFood Chem X
August 2025
School of Life Science, Anqing Normal University, Jixian North Road1318, Yixiu District, Anqing 246052, Anhui Province, China.
Frozen storage deteriorates the texture and digestibility of frozen rice dough by damaging gliadin structure and starch integrity. This study investigated carboxymethyl chitosan (CMCh) and sodium carboxymethyl cellulose (CMCNa) as cry-oprotectants to mitigate these effects. Comprehensive analysis utilizing nuclear magnetic resonance (NMR), texture profile analysis (TPA), dynamic contact angle measurement (DCAT21), reversed-phase high-performance liquid chromatography (RP-HPLC), and circular dichroism (CD) demonstrated that 1.
View Article and Find Full Text PDFBiotechniques
September 2025
Woman, Mother + Baby Research Institute, Tufts Medicine, Boston, MA, USA.
MicroRNAs (miRNAs) are considered more stable than mRNA, but the impact of progressive thawing of biological samples after freezing as may happen during shipping delays has not been quantified. To address this, we utilized digital PCR to estimate the absolute concentrations of select miRNAs following progressive thawing of human plasma and maintenance at ambient temperature. Specifically, we quantified let-7b-3p, miR-144-5p, miR-150-5p, miR-517a-3p, miR-524-5p, and miR-1283, which have varying abundance in plasma.
View Article and Find Full Text PDFJ Microbiol Methods
September 2025
Dynamics of Respiratory Infections Group, Helmholtz Centre for Infection Research-HZI Braunschweig, Braunschweig, Germany; Department of Respiratory Medicine and Infectious Diseases, Hannover Medical School, German Center for Lung Research (DZL), BREATH, Hannover, Germany.
Purpose: The accuracy of oral microbiome research depends significantly on specimen sampling protocols, as well as their storage and preservation. Traditional methods, such as freezing, may not only involve logistical hurdles but can also impact the quality of microbial data, leading to difficulties in the comparability between different studies. This study evaluates the effectiveness of the room temperature nucleic acid preservation protocol using DNA/RNA Shield buffer as compared to standard freezing in preserving oral microbial communities over the course of 7 days.
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