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In this work, a photobioreactor with microalgae biofilm was proposed to enhance CO biofixation and protein production using nickel foam with the modified surface as the carrier for immobilizing microalgae cells. The results demonstrated that, compared with microalgae suspension, microalgae biofilm lowered mass transfer resistance and promoted mass transfer efficiency of CO from the bubbles into the immobilized microalgae cells, enhancing CO biofixation and protein production. Moreover, parametric studies on the performance of the photobioreactor with microalgae biofilm were also conducted. The results showed that the photobioreactor with microalgae biofilm yielded a good performance with the CO biofixation rate of 4465.6 µmol m s, the protein concentration of effluent liquid of 0.892 g L, and the protein synthesis rate of 43.11 g m h. This work will be conducive to the optimization design of microalgae culture system for improving the performance of the photobioreactor.
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http://dx.doi.org/10.1007/s00449-018-2055-4 | DOI Listing |
Water Res
August 2025
MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, PR China.
Microalgal-bacterial biofilm could realize synergistic pollutants removal, CO sequestration, and resource transformation from wastewater. Pre-designed biofilm with clear microbial composition would benefit resource transformation, yet little is known about its nutrients removal performance under axenic conditions, not to mention the comparison with non-axenic conditions over extended operation. To fill in this knowledge gap, this study first investigated the growth characteristics and nutrients removal performances of a pre-designed microalgae dominant biofilm.
View Article and Find Full Text PDFEnviron Pollut
August 2025
Universite de Pau et des Pays de L'Adour, E2S-UPPA, CNRS, IPREM, Pau, France.
Biofilms are integral to the biogeochemical cycles of aquatic ecosystems, primarily through complex interactions among microorganism that mediate the transformation and movement of key elements. In a previous study, we reported that Cyanobacteria within biofilms grown in outdoor mesocosms exhibited sensitivity to cobalt (Co) when exposed to increasing Co concentrations (background concentrations, 0.1, 0.
View Article and Find Full Text PDFBiofabrication
August 2025
Marine Biological Section, Department of Biology, University of Copenhagen, Strandpromenaden 5, Helsingør, DK 3000, Denmark.
The intricate three dimensional architecture at different spatial length scales affects the functionality and growth performance of immobilized photosynthesizing cells in biofilms and bioprinted constructs. Despite the tremendous potential of 3D bioprinting in precisely defining sample heterogeneity and composition in spatial context, cell metabolism is mostly measured in media surrounding the constructs or by destructive sample analyzes. The exploration and application of non-invasive techniques for monitoring physico-chemical microenvironments, growth and metabolic activity of cells in 3D printed constructs is thus in strong demand.
View Article and Find Full Text PDFSheng Wu Gong Cheng Xue Bao
July 2025
College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
Microalgae possess high photosynthetic efficiency, robust adaptability, and substantial biomass, serving as excellent biological resources for large-scale cultivation. Malic enzyme (ME), a ubiquitous metabolic enzyme in living organisms, catalyzes the decarboxylation of malate to produce pyruvate, CO, and NAD(P)H, playing a role in multiple metabolic pathways including energy metabolism, photosynthesis, respiration, and biosynthesis. In this study, we identified the malic enzyme gene () and its biological functions, aiming to provide excellent target genes for the genetic improvement of higher plants.
View Article and Find Full Text PDFMar Biotechnol (NY)
August 2025
Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Instituto Andaluz de Biotecnología y Desarrollo Azul (IBYDA), Campus Universitario de Teatinos S/N, 29071, Málaga, Spain.
Vibrio proteolyticus DCF12.2 has demonstrated its ability to be used as a probiotic for fish species. This study investigates how different culture conditions influence the activity of its extracellular products (ECPs) in aquaculture, focusing on enzymatic and antibacterial activity, cytotoxicity, biofilm modulation, short-chain fatty acid (SCFA) profiles, and effects on Photobacterium damselae subsp.
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