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Watermelon juice was exposed to the enzyme masazyme at varying enzyme concentrations (0.01-0.1 % w/w) and different time (20-120 min) and temperature (30-50 °C) combinations. The effects of the treatments on selected responses (juice recovery, total dissolved solids (TDS), viscosity, turbidity, cloud stability and L value) were determined employing a second order Box Behnken Design in combination with Response Surface Methodology. Enzymatic treatment effectively degraded polysaccharides, resulting in reduced viscosity, turbidity and absorbance value and increased juice recovery, total dissolved solids and lightness. R(2) value for all models for the dependent variables were greater than 90 %. The maximum juice recovery (86.27 %), TDS (8.7°Brix) and L value (17.57) while minimum viscosity (0.0020 Pa.s.), turbidity (39.37 NTU) and cloud stability (0.033 abs) were obtained when enzyme treatment was set up with 0.09 % w/w enzyme concentration at 46.90 °C and 117.45 min.
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http://dx.doi.org/10.1007/s13197-012-0720-1 | DOI Listing |
Mikrochim Acta
September 2025
Faculty of Science, Shenyang University of Chemical Technology, Shenyang, 110142, China.
A sensitive electrochemical glucose biosensor using ZrO₂@CNTs nanocomposite was developed for real-time metabolism monitoring for athletes. The nanocomposite was prepared by a simple ultrasound-assisted technique, and the glucose oxidase (GOx) was covalently immobilized to improve the biorecognition ability. CNTs treated with acid served as a highly conductive framework, and ZrO₂ nanoparticles can provide structural stability and catalytic performance, thus showing synergistic enhancement of electron transfer kinetics and enzyme loading capacity.
View Article and Find Full Text PDFTalanta
September 2025
Department of Physical and Environmental Sciences, University of Toronto, Scarborough, Toronto, Ontario, M1C 1A4, Canada. Electronic address:
An one-pot method was used to prepare bimetallic nanozymes, with chitosan (CS) and l-tyrosine (L-Tyr) as stabilized dispersed colloidal solutions and a carrier for gold-platinum single atoms (Au-Pt SAs), which exhibited excellent peroxidase activity. A colorimetric method based on CS/L-Tyr/Au-Pt SAs nanozymes was constructed for the colorimetric detection of quercetin (QR) in human serum and orange juice. The synthesized bimetallic nanozymes were characterized by SEM, TEM, HAADF-STEM, FT-IR, XRD and XPS techniques to demonstrate the successful synthesis of CS/L-Tyr/Au-Pt SAs nanozymes.
View Article and Find Full Text PDFAnal Chem
September 2025
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China.
Covalent organic frameworks (COFs) exhibit exceptional adsorption capacity but suffer from challenging desorption. In this study, a bromine-functionalized magnetic COF composite (FeO@COF-Br) was synthesized via bromodimethylsulfonium bromide-mediated bromination of imine-linked COF on FeO nanospheres (FeO@COF), where the COF framework was constructed by 1,3,5-tris(4-formylphenyl)benzene and 2,6-diamino-3,5-diethynylpyridine. Compared with pristine FeO@COF, FeO@COF-Br enhanced desorption efficiencies for brominated contaminants by about 30% while maintaining adsorption capacity.
View Article and Find Full Text PDFFood Microbiol
January 2026
College of Food Science and Engineering, Northwest University, Xi'An, 710069, Shaanxi, China; Laboratory of Nutritional and Healthy Food-Individuation Manufacturing Engineering, Xi'An, 710069, Shaanxi, China; Research Center of Food Safety Risk Assessment and Control, Xi'An, 710069, Shaanxi, China.
The protein kinase Hog1 plays a central role in cellular responses, including cell volume and gene expression regulation during osmoregulation in the model yeast Saccharomyces cerevisiae. Despite sharing the conserved kinase Hog1 for osmotic response, Zygosaccharomyces rouxii and S. cerevisiae exhibit markedly different sugar resistance.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
August 2025
Institute of Biomedical Precision Testing and Instrumentation, College of Artificial Intelligence, Taiyuan University of Technology, Jinzhong 030600, Shanxi, China. Electronic address:
Excessive pesticide residues have posed a severe risk to human health and ecological environment, making stringent testing of them a crucial step in food safety supervision. Herein, a flexible paper-based SERS platform composed of AgNPs and MoS nanoflowers was developed for the rapid and ultra-sensitive detection of thiram in juice. Among it, the hydrophobic MoS-modified paper was first fabricated, followed by co-depositing small-sized AgNPs and thiram molecules onto its surface for SERS analysis.
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