[The structure and phosphorus or potassium deficiency induced expression of a calmodulin-like protein gene in Arabidopsis].

Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao

The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310029, China.

Published: October 2005


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Article Abstract

According to our previous microarray analysis, we found a putative calmodulin gene related to Pi deficiency and designated AtPsiCaM (Arabidopsis Pi-starvation-induced CaM). Results of structural analysis indicate that AtPsiCaM has three conserved EF-hands motif and belongs to calmodulin-like proteins family (Figs. 1-3). Northern blot analysis revealed that this gene could be induced by potassium and phosphate deficiency and not by potassium deficiency or high salinity (Fig. 4). The results of RT-PCR and GUS histochemical staining assays of the AtPsiCaM promoter::GUS transgenic plants showed that this gene can be expressed in all tissues to different expression levels (Figs. 5, 6).

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