Fine mapping of the dominant female sterility gene and novel model of hybrid seed production in cabbage (Brassica oleracea L. var. capitata).

Hybrid breeding based on male sterility requires the removal of male parents, which is time- and labor-intensive; however, the use of female sterile male parent can solve this problem. In the offspring of distant hybridization between Brassica oleracea and Brassica napus, we obtained a mutant, 5GH12-279, which not only fails to generate gynoecium (thereby causing female sterility) but also has serrated leaves that could be used as a phenotypic marker in seedling screening. Genetic analysis revealed that this trait was controlled by a single dominant gene.

Multi-Time Point Transcriptome Analysis and Functional Validation Revealed Negatively Regulates Black Rot Resistance in Cabbage.

4-coumarate-CoA ligase (4CL) plays a crucial role in the phenylpropanoid metabolic pathway and is a key enzyme involved in plant growth and stress responses. Black rot, caused by pv. () is a major bacterial disease affecting the production of global cruciferous crop-like cabbage ( var.

Rapid design of transgene-free cabbage with desired anthocyanin contents via HI-Edit.

The HI-Edit system combines haploid induction and CRISPR/Cas-based genome editing to provide a promising way to design crops with desired traits in a rapid, precise and transgene-free manner. HI-Edit was applied to produce cabbages with desired anthocyanin contents.

Clubroot resistant in cruciferous crops: recent advances in genes and QTLs identification and utilization.

Clubroot, caused by , poses a serious threat to cruciferous crop production worldwide. Breeding resistant varieties remains the most cost-effective strategy to mitigate yield losses, yet achieving durable, stable, and broad-spectrum resistance continues to be a formidable challenge. Recent advances in genetic and genomic technologies have improved the understanding of complex host-pathogen interactions, leading to the identification of key resistance loci, including dominant resistance genes such as and , as well as quantitative trait loci.

Research on the Layout Design of Auxiliary Support Modules for Suppressing Machining Chatter in Thin-Walled Beams.

A well-designed clamping layout significantly enhances the dynamic stiffness of a manufacturing system, improving its stability and suppressing cutting chatter in workpieces. This paper focuses on the machining of thin-walled beams, which are prone to vibration and have low stiffness, especially under hydraulic floating clamping conditions. By analyzing the system stability domain, we propose a method to improve system stiffness through strategic design of support module layouts.

Transcriptome Analysis of Cabbage Near-Isogenic Lines Reveals the Involvement of the Plant Defensin Gene in Wilt Resistance.

wilt of cabbage ( var. ), caused by f. sp.

Transcriptome and Metabolome Analyses Offer New Insights into Bolting Time Regulation in Broccoli.

The globular buds and stems are the main edible organs of broccoli. Bolting is an important agronomic trait, and the timing of its occurrence is particularly critical when breeding and domesticating broccoli. The molecular mechanism that regulates broccoli bolting time is not well-understood.

Genome-Wide Characterization of / Gene Family in Cabbage ( var. ): Expression Profiling and Functional Implications During Infection.

Calmodulin (CaM) and calmodulin-like proteins (CMLs) are crucial for calcium signal transduction in plants. Although / genes have been extensively studied in various plant species, research on these genes in is still limited. In this study, 14 and 75 genes were identified in the genome through a genome-wide search.

Genome-wide identification of the ECERIFERUM (CER) gene family in cabbage and critical role of BoCER4.1 in wax biosynthesis.

The ECERIFERUM (CER) gene family is essential for the biosynthesis of plant cuticular wax. In this study, 32 BoCER genes were identified in cabbage through genome-wide analysis. We found that the BoCER genes are highly conserved with their homologous counterparts in Arabidopsis thaliana.

Two large inversions seriously suppress recombination and are essential for key genotype fixation in cabbage ( L. var. ).

Chromosomal inversion is an important structural variation that usually suppresses recombination and is critical for key genotype fixation. In a previous study, an 11.47 Mb recombination suppression region was identified in the yellow-green leaf locus on chromosome 1, but the cause of recombination suppression is still unclear.

Generation of novel bpm6 and dmr6 mutants with broad-spectrum resistance using a modified CRISPR/Cas9 system in Brassica oleracea.

Using an optimized CRISPR/Cas9 system to knock out the BTB-POZ and MATH domain gene BoBPM6 and the DOWNY MILDEW RESISTANCE 6 gene in Brassica oleracea resulted in new lines with broad-spectrum disease resistance.

Rapid introgression of the clubroot resistance gene into cabbage skeleton inbred lines through marker assisted selection.

Unlabelled: Clubroot, caused by , is a globally pervasive soil-borne disease that poses a significant challenge primarily in cruciferous crops. However, the scarcity of resistant materials and the intricate genetic mechanisms within cabbage present major obstacles to clubroot resistance (CR) breeding. In our previous research, we developed an Ogura CMS cabbage variety, "17CR3", which harbors the gene, crucial for CR.

Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants.

This review provides a comprehensive summary of the latest international research on detection methods for glucosinolates in cruciferous plants. This article examines various analytical techniques, including high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), enzyme-linked immunosorbent assay (ELISA), and capillary electrophoresis (CE), while highlighting their respective advantages and limitations. Additionally, this review delves into recent advancements in sample preparation, extraction, and quantification methods, offering valuable insights into the accurate and efficient determination of glucosinolate content across diverse plant materials.

Fine mapping and identification of the downy mildew resistance gene BoDMR2 in Cabbage (Brassica oleracea L. var. capitata).

Background: Cabbage (Brassica oleracea L. var. capitata) is an important crop within the Brassica oleracea species and is extensively cultivated worldwide.

Optimization Method of Floating Fixture Layout for Distortion Control of Low-Stiffness Thin-Walled Beams.

The aim is to reduce the elastic deformation of the web and side walls of low-stiffness thin-walled beams when the floating fixture method is used. This paper takes the number and position of fixture points as the optimization variables, establishes a calculation model of elastic deformation, and constructs the objective function of maximum total elastic deformation. An optimized solution utilizing the augmented multiplier method is employed, which forms the basis for the fixture layout optimization method to reduce the elastic deformation of low-stiffness thin-walled beams.

Neuroprotective Effects of Metformin on Cerebral Ischemia-Reperfusion Injury: Modulation of JNK and p38 MAP Kinase Signaling Pathways.

Cerebral ischemia-reperfusion injury (CIRI) is a significant pathological process in stroke, characterized by neuronal cell death and neurological dysfunction. Metformin, commonly used for diabetes management, has been noted for its neuroprotective properties, though its effects on CIRI and the mechanisms involved remain unclear. This study explored the neuroprotective impact of metformin on CIRI, focusing on its potential to modulate the c-Jun N-terminal kinase (JNK) and p38 MAP kinase (p38) signaling pathways.

Chloroplast C-to-U editing, regulated by a PPR protein BoYgl-2, is important for chlorophyll biosynthesis in cabbage.

Leaf color is an important agronomic trait in cabbage ( L. var. ), but the detailed mechanism underlying leaf color formation remains unclear.

Plant non-coding RNAs: The new frontier for the regulation of plant development and adaptation to stress.

Most plant transcriptomes constitute functional non-coding RNAs (ncRNAs) that lack the ability to encode proteins. In recent years, more research has demonstrated that ncRNAs play important regulatory roles in almost all plant biological processes by modulating gene expression. Thus, it is important to study the biogenesis and function of ncRNAs, particularly in plant growth and development and stress tolerance.

Large-scale gene expression alterations introduced by structural variation drive morphotype diversification in Brassica oleracea.

Brassica oleracea, globally cultivated for its vegetable crops, consists of very diverse morphotypes, characterized by specialized enlarged organs as harvested products. This makes B. oleracea an ideal model for studying rapid evolution and domestication.

Transcriptome Analysis Reveals Key Genes and Pathways Associated with the Regulation of Flowering Time in Cabbage ( L. var. ).

Flowering time is an important agronomic trait in cabbage ( L. var. ), but the molecular regulatory mechanism underlying flowering time regulation in cabbage remains unclear.

A Whole-Genome Assembly for , A Pathogen Causing Downy Mildew in Cabbage ( var. L.).

is a global pathogen that can cause leaf necrosis and seedling death, severely threatening the quality and yield of cabbage. However, the genome sequence and infection mechanisms of are still unclear. Here, we present the first whole-genome sequence of isolate BJ2020, which causes downy mildew in cabbage.

Introgression of clubroot resistant gene into L. from based on homoeologous exchange.

Clubroot is a soil-borne disease in cabbage ( L. var. L.

Comparative Transcriptome Analysis Reveals a Potential Regulatory Network for Ogura Cytoplasmic Male Sterility in Cabbage ( L.).

Ogura cytoplasmic male sterility (CMS) lines are widely used breeding materials in cruciferous crops and play important roles in heterosis utilization; however, the sterility mechanism remains unclear. To investigate the microspore development process and gene expression changes after the introduction of and , cytological observation and transcriptome analysis were performed using a maintainer line, an Ogura CMS line, and a restorer line. Semithin sections of microspores at different developmental stages showed that the degradation of tapetal cells began at the tetrad stage in the Ogura CMS line, while it occurred at the bicellular microspore stage to the tricellular microspore stage in the maintainer and restorer lines.

Glucosinolate Biosynthetic Genes of Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis.

Cabbage ( var. ) is a vegetable rich in glucosinolates (GSLs) that have proven health benefits. To gain insights into the synthesis of GSLs in cabbage, we systematically analyzed GSLs biosynthetic genes (GBGs) in the entire cabbage genome.

protects against ischemic stroke by targeting .

Background: Ischemic stroke is a brain dysfunction disease caused by vascular obstruction. The expression of many kinds of microRNAs (miRNAs) is related to ischemic stroke. MiRNA has the ability to reduce or save ischemic injury.