Host-Gated Enzymatic Release (H-GER) Enables Colorimetric Transduction for Enzyme Measurement.

We introduce Host-Gated Enzymatic Release (H-GER) as an alternative colorimetric signal transduction mechanism for measuring amylase activity. This assay uses a visually colored complex formed when hydroxypropyl-γ-cyclodextrin (HP-γ-CD) binds to the aggregachromic dye CRANAD-2, with the HP side chains playing a key role in the complexation. The analytical capability of this visually addressable assay relies on changes in dye dispersity, triggered by the enzymatic release of gated CRANAD-2 from HP-γ-CD host.

Post-Acupuncture Acute Cervical Spinal Epidural Hematoma With Hemiplegia Misdiagnosed as Cerebral Infarction: A Case Report and Literature Review.

This case report describes a rare but serious complication termed post-acupuncture acute cervical spinal epidural hematoma (paACSEH). The patient presented with right-sided hemiplegia and cervical pain following acupuncture therapy. Initial misdiagnosis as cerebral infarction led to inappropriate alteplase thrombolytic therapy, which was promptly discontinued after CT imaging confirmed cervical spinal epidural hematoma.

High dynamic range fringe projection profilometry with binary fringes differencing demodulation.

Fringe projection profilometry (FPP) using gray-scale patterns like sinusoidal stripes often encounters challenges for high dynamic range (HDR) applications. These challenges arise due to the presence of shiny surfaces or abrupt changes in reflectivity, leading to either reconstruction failure or substantial errors. We present a robust modulation and reconstruction method that divides the grayscale sinusoidal fringe into eight binary fringes and employs a differencing approach to mitigate HDR surface interference and enable demodulation, significantly reducing vulnerability to reflectivity fluctuations.

Inhibitory effects of herbal monomers on ferroptosis in renal fibrosis: a review and mechanistic study.

Background And Purpose: Renal fibrosis is a common characteristic of chronic kidney disease (CKD). Studies have confirmed the role of ferroptosis in the pathogenesis of various kidney diseases, making it a new research hotspot in the field of renal fibrosis. Monomers of Chinese herbal medicines (CHMs) can improve renal fibrosis by multi-target inhibition of ferroptosis.

The effect of Ti doping on He migration behavior in β-TMH (TM = Sc and Er): first-principles calculations.

In this work, the stability and migration behavior of He in Ti doped β-TMH (TM = Sc and Er) are investigated by using density functional theory (DFT) calculations. The addition of Ti atoms reduces the formation energy of He in TMH (TM = Sc and Er). Ti atoms repel He atoms in TMH (TM = Sc and Er), reducing the stability of He in the interstitial sites.

The role of mA RNA methylation in a love-hate relationship between porcine rotavirus and host cells.

N6-methyladenosine (mA), the most abundant mRNA modification, regulates various mRNA metabolism to affect numerous physiological processes, including immune response. Interestingly, many RNA viruses contain internal mA modifications that contribute to viral replication and innate immune escape process, but its mechanisms remain unclear. Porcine rotavirus (PoRV) is a common cause of diarrhea and gastroenteritis in piglets.

Immune stress impairs broiler performance by affecting hypothalamic methylation modification and intestinal 5-HT synthesis.

In this experiment, a chronic immune stress model was established in broiler chickens by injecting lipopolysaccharide (LPS). The objective was to investigate the impact of immune stress on performance and intestinal mucosal barrier function in broiler chickens and identify key molecular and neuroendocrine pathways contributing to these effects. We randomly divided 160 one-day-old male white-feathered broilers into two groups: the control group (CON) and the LPS immune stress group (LPS), with 8 replicates of 10 chickens each.

The influence of hydrogel stiffness on axonal regeneration after spinal cord injury.

The core challenge in spinal cord injury(SCI) treatment is promoting axonal regeneration and forming new neural connections in damaged areas. However, mature CNS neurons have limited regenerative capacity, causing long-term dysfunction. Axonal regeneration involves elongating axons guided by growth cones, which sense and respond to external mechanical signals, integrating them into cytoskeleton reconstruction.

N-methyladenosine reader YTHDF3-mediated CEBPA translation maintains genomic stability and stem cell function to prevent liver injury.

Liver injury is a major health issue with significant implications for liver function and overall well-being, but precise mechanisms of the N-methyladenine (mA) reader YTHDF3 in liver injury remain severely understudied. Here, we discovered that Ythdf3 knockout exacerbated CCL-induced liver injury with a reduction in functional hepatocytes and liver stem cells using single cell RNA-sequencing and organoid culture. Furthermore, Mettl14 and YTHDF3-dependent RNA mA dysregulation induced DNA damage.

Dual-Signal Probing of Molecular Subtypes of Breast Cancer: Synergistic Chirality and Charge-Transfer Effect Enable Enhanced Accuracy.

Tumor molecular subtyping is essential for guiding personalized treatment strategies. However, existing detection methods are constrained by complex procedures, prolonged processing times, and high costs. Therefore, the development of rapid and straightforward detection methods remains a critical unmet need.

Polydimethylsiloxane-Zinc Oxide Nanorod-Based Triboelectric Nanogenerator for Compression Applications.

In this study, to enhance the output performance of a contact-separation mode triboelectric nanogenerator (TENG), a zinc oxide nanorod (ZnO NR) film with piezoelectric properties was integrated into a Polydimethylsiloxane (PDMS) film as the dielectric layer. The working mechanism of the PDMS-ZnO NR-based TENG was theoretically analyzed in two stages: charge transfer during contact electrification on the material surface and charge movement in the electrostatic induction process. The output characteristics of the PDMS-ZnO NR-based TENG were investigated and compared with those of a PDMS-based TENG.

Pathogenesis of Focal Segmental Glomerulosclerosis Caused by a Leu754Val Mutation in ARHGAP32.

Focal segmental glomerulosclerosis (FSGS) shows a poor response to hormones and other treatment schemes and rapidly progresses to end-stage renal disease. Genetic factors are important causes of FSGS. We recently identified a new candidate pathogenic ARHGAP32 mutation in a family affected by FSGS and further investigated its functional impact through in vivo and in vitro studies.

Identification of Nuclear Localization Sequence (NLS) Sites in R2R3-MYB Transcription Factor Involved in Anther Development.

The R2R3-MYB family of transcription factors (TFs) plays a crucial role in cell specification and secondary metabolism regulation during plant development. In Arabidopsis, MS188, a typical R2R3-MYB protein, is essential for tapetal development and pollen wall formation. However, the nuclear localization sequence (NLS) responsible for directing MS188 into the nucleus has not been fully elucidated.

Interaction between energy level and starch:fat ratio on intestinal energy metabolism of layer pullets.

During the growing period, the gastrointestinal tract of layer pullets is not yet well developed and may be susceptible to dietary energy level. The energy level and composition might impact the intestinal energy metabolism of layer pullets. To test this hypothesis, a total of 480 "Jing Tint 6" layer pullets were used in an 8-week study and allocated to 4 groups, each consisting of 8 replicates, with 15 birds per replicate.

Preoperatively predicting failure to achieve the minimum clinically important difference and the substantial clinical benefit in patient-reported outcome measures for total hip arthroplasty patients using machine learning.

Background: Attention to the collection of patient-reported outcomes measures (PROMs) associated with total hip arthroplasty (THA) is growing. The aim of this study was to preoperatively predict failure to achieve the minimal clinically important difference (MCID) and substantial clinical benefit (SCB) between pre- and postoperative PROMs. In addition, we sought to identify factors predictive of failure to achieve MCID and SCB in patients undergoing a THA.

Integration of metabolomics and transcriptomics reveals the mechanism of TMEM30A downregulation induced FSGS podocyte injury.

Podocyte injury plays a critical role in the pathogenesis and progression of focal and segmental glomerulosclerosis (FSGS). Transmembrane protein 30 A (TMEM30A) downregulation participates in podocyte injury. This study aimed to identify the critical pathways and molecules associated with the downregulation of TMEM30A in the context of FSGS podocyte injury.

Using Large Language Models to Detect and Understand Drug Discontinuation Events in Web-Based Forums: Development and Validation Study.

Background: The implementation of large language models (LLMs), such as BART (Bidirectional and Auto-Regressive Transformers) and GPT-4, has revolutionized the extraction of insights from unstructured text. These advancements have expanded into health care, allowing analysis of social media for public health insights. However, the detection of drug discontinuation events (DDEs) remains underexplored.

INFORMING INTENSIVE CARE UNIT DIGITAL TWINS: DYNAMIC ASSESSMENT OF CARDIORESPIRATORY FAILURE TRAJECTORIES IN PATIENTS WITH SEPSIS.

Understanding clinical trajectories of sepsis patients is crucial for prognostication, resource planning, and to inform digital twin models of critical illness. This study aims to identify common clinical trajectories based on dynamic assessment of cardiorespiratory support using a validated electronic health record data that covers retrospective cohort of 19,177 patients with sepsis admitted to intensive care units (ICUs) of Mayo Clinic Hospitals over 8-year period. Patient trajectories were modeled from ICU admission up to 14 days using an unsupervised machine learning two-stage clustering method based on cardiorespiratory support in ICU and hospital discharge status.

Corrigendum to 'Machine Learning-Based Predictive Models for 90-Day Readmission of Total Joint Arthroplasty Using Comprehensive Electronic Health Records and Patient-Reported Outcome Measures'. [Arthroplasty Today Volume 25, February 2024, 101308].

[This corrects the article DOI: 10.1016/j.artd.

Investigation of the causal relationship between patient portal utilization and patient's self-care self-efficacy and satisfaction in care among patients with cancer.

Objective: The objective of this study was to examine the causal relationship between the usage of patient portals and patients' self-care self-efficacy and satisfaction in care outcomes in the context of cancer care.

Methods: The National Institute's HINTS 5 Cycle 1-4 (2017-2020) data were used to perform a secondary data analysis. Patients who reported being ever diagnosed with cancer were included in the study population.

Ferulic Acid Alleviates Lipid and Bile Acid Metabolism Disorders by Targeting FASN and CYP7A1 in Iron Overload-Treated Mice.

Iron overload is a common complication in various chronic liver diseases, including non-alcoholic fatty liver disease (NAFLD). Lipid and bile acid metabolism disorders are regarded as crucial hallmarks of NAFLD. However, effects of iron accumulation on lipid and bile acid metabolism are not well understood.

Ketamine attenuates kidney damage and depression-like behaviors in mice with cisplatin-induced acute kidney injury.

Acute kidney injury (AKI) is a serious condition characterized by decreased urine output, often accompanied by psychiatric symptoms like depression. However, there are limited pharmacological treatments available for AKI and its associated depressive symptoms. In this study, we investigated whether cisplatin-induced AKI in mice leads to depression-like behaviors and whether ketamine could alleviate both the kidney injury and these behaviors.