Melatonin Implantation Improves the Reproductive Performance of Estrus-Synchronized Ewes During Seasonal Anestrus and Enhances the Antioxidant and Steroidogenic Capacities of Granulosa and Luteal Cells.

Seasonal reproduction in sheep reduces reproductive efficiency. Melatonin (MT) plays a crucial role in reproductive processes. The purpose of this study was to assess the effects of a 5-day MT implant pretreatment on estrus synchronization and reproductive performance in sheep during seasonal anestrus.

Compulsive coping behaviour, developed predominantly by sign-trackers, is exacerbated by chronic atomoxetine.

Background: Loss of control over coping strategies can result in the development of impulsive/compulsive spectrum disorders (ICSDs) such as obsessive-compulsive disorder or trichotillomania. Rats, like humans, show individual differences in their tendency to engage in and maintain control over coping behaviours. While most rats exposed to a schedule-induced polydipsia (SIP) procedure develop controlled, moderate, polydipsic coping, some vulnerable individuals engage in excessive, compulsive drinking, or hyperdipsia.

Relationship between elevated habituation tendencies and impulsivity, compulsivity, and childhood adversity among abstinent patients with methamphetamine use disorders.

Elevated habituation tendencies have been identified in several subgroups of patients with substance use disorders, however, their intricate relationship with substance use disorders remains unknown. A total of 133 individuals with methamphetamine use disorder (PwMUD) and 131 healthy controls (HC) were initially recruited for this study. After exclusion of outliers, data from 128 PwMUD and 125 HC were included in the analyses.

Development of a novel method of biochemical oxygen demand colorimetric detection and its application in actual water.

In this study, a novel and efficient colorimetric biosensor was constructed for biochemical oxygen demand (BOD) detection based on visual analysis and Qualcomm measurement with enzyme-labeling instrument. Microorganisms cultured in situ with actual water and Prussian blue were used as model bacteria and indicator of colorimetric biosensor, respectively. To accurately detect BOD content in water, the reaction conditions such as cell concentration, K[Fe(CN)] concentration, reaction temperature, and reaction time were optimized.

Structural and functional disconnections in non-acute post-stroke patients.

Background: Structural alterations and functional reorganizations related to motor dysfunction after stroke remain unclear. This study aims to investigate alterations in structural connectivity (SC) and functional connectivity (FC) in non-acute post-stroke patients, and their associations with motor performance.

Methods: Thirty-six non-acute post-stroke patients and thirty-eight well-matched healthy controls (HCs) were included.

Fluorescent Nanozyme Based on Bimetallic Fe/Co-Doped Carbon Dots for Dual-Mode Detection of Ascorbic Acid and Hydrogen Peroxide.

Despite extensive research on nanozymes in analytical sensing, single-mode detection strategies remain constrained by reliability challenges. We developed bimetallic Fe/Co-doped carbon dots nanozymes (FeCo-CDs) for dual-mode (colorimetric/fluorescent) detection of ascorbic acid (AA) and hydrogen peroxide (HO). The synergistic Fe/Co codoping strategy generates abundant catalytically active sites, significantly enhancing peroxidase (POD)-like activity while simultaneously amplifying intrinsic fluorescence.

NiFe-LDH/BiVO-BOD Photocatalytic Fuel Cell for Bias-Free and Selective 5-Hydroxymethylfurfural Oxidation to 2,5-Furandicarboxylic Acid.

The efficient conversion of renewable biomass into value-added products is essential for sustainable development. In this work, we report a two-chamber photocatalytic fuel cell (PFC) consisting of a NiFe-LDH/BiVO/FTO photoanode and a BOD/TCPP/BP/ITO biocathode, which allows for the simultaneous accomplishment of energy conversion and bias-free production of 2,5-furandicarboxylic acid (FDCA). After optimization, the PFC achieves a maximum power density of 859.

Enhanced electrochemical performance of N-doped carbon coated NaFePOF cathode materials for sodium-ion batteries: achieving high capacity and cycle stability.

Iron-based phosphates, known for their abundance, cost-effectiveness, and eco-friendliness, have garnered significant attention as viable cathode materials for sodium-ion batteries. However, synthesizing pure phase, high-capacity cathodes remains a substantial challenge. This research focuses on NaFePOF and achieves a significant enhancement in its electrochemical properties through a refined preparation process, leading to the development of NaFePOF@C.

Harnessing Infinite Coordination Polymers to Eliminate Harmful Environmental DNA for Water Purification.

Harmful environmental DNA (eDNA) contamination in water poses significant health risks, yet traditional treatments fall short of achieving efficient removal. Here, we introduce 1MIA-Zr, a bioinspired infinite coordination polymer catalyst modeled after natural nucleases. 1MIA-Zr achieves a DNA hydrolysis rate approximately 40 times greater than that of UiO-66 REO, the previous best zirconium-based catalyst.

High-Valence Mn MOF Inspired by Laccase Mediators Enables Versatile Nature-Mimicking Catalysis.

In nature, active Mn -ligand complexes produced by laccase catalyzed oxidation can act as the low-molecular mass, diffusible redox mediators to oxidize the phenolic substrates overcoming the limitations of natural enzymes. Learning from the metal-ligand coordination of natural functional units, high-valence Mn metal-organic framework (Mn MOF) is constructed to simulate the catalysis in natural mediator system. Benefiting from the characteristics of nanoscale size, rich metal coordination unsaturated sites, and mixed valence state dominated by Mn(III), Nano Mn(III)-TP exhibits superior laccase-mimicking activity, whose V (maximal reaction rate) is much higher than that of natural laccase.

3,6-Dimethoxythieno[3,2-b]thiophene-Based Bifunctional Electrodes for High-Performance Electrochromic Supercapacitors Prepared by One-Step Electrodeposition.

An integrated visual energy system consisting of conjugated polymer electrodes is promising for combining electrochromism with energy storage. In this work, we obtained copolymer bifunctional electrodes poly(3,6-dimethoxythieno[3,2-b]thiophene--2,3-dihydrothieno[3,4-b][1,4]dioxin-3-ylmethanol)(P(TT-OMe--EDTM)) by one-step electrochemical copolymerization, which exhibits favorable electrochromic and capacitive energy storage properties. Because of the synergistic effect of PTT-OMe and PEDTM, the prepared copolymers show better flexibility.

Cell Wall Binding Strategies Based on CuSbS Nanoparticles for Selective Bacterial Elimination and Promotion of Infected Wound Healing.

Utilizing nanomaterials as an alternative to antibiotics, with a focus on maintaining high biosafety, has emerged as a promising strategy to combat antibiotic resistance. Nevertheless, the challenge lies in the indiscriminate attack of nanomaterials on both bacterial and mammalian cells, which limits their practicality. Herein, CuSbS nanoparticles (NPs) capable of generating reactive oxygen species (ROS) are discovered to selectively adsorb and eliminate bacteria without causing obvious harm to mammalian cells, thanks to the interaction between O of N-acetylmuramic acid in bacterial cell walls and Cu of the NPs.

A Multi-view Molecular Pre-training with Generative Contrastive Learning.

Molecular representation learning can preserve meaningful molecular structures as embedding vectors, which is a necessary prerequisite for molecular property prediction. Yet, learning how to accurately represent molecules remains challenging. Previous approaches to learning molecular representations in an end-to-end manner potentially suffered information loss while neglecting the utilization of molecular generative representations.

S-Doping Regulated Iron Spin States in Fe-N-C Single-Atom Material for Enhanced Peroxidase-Mimicking Activity at Neutral pH.

Designing biomimetic nanomaterials with peroxidase (POD)-like activity at neutral pH remains a significant challenge. An S-doping strategy is developed to afford an iron single-atom nanomaterial (Fe@CN-S) with high POD-like activity under neutral conditions. To the best of knowledge, there is the first example on the achievement of excellent POD-like activity under neutral conditions by regulating the active site structure.

Nanozyme sensor array based on Fe, Se co-doped carbon material for the discrimination of Sulfur-containing compounds.

Developing methods for the accurate identification and analysis of sulfur-containing compounds (SCCs) is of great significance because of their essential roles in living organisms and the diagnosis of diseases. Herein, Se-doping improved oxidase-like activity of iron-based carbon material (Fe-Se/NC) was prepared and applied to construct a four-channel colorimetric sensor array for the detection and identification of SCCs (including biothiols and sulfur-containing metal salts). Fe-Se/NC can realize the chromogenic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by activating O without relying on HO, which can be inhibited by different SCCs to diverse degrees to produce different colorimetric response changes as "fingerprints" on the sensor array.

CuFe Layered Double Hydroxide as Self-Cascade Nanoreactor for Efficient Antibacterial Therapy.

Nanozyme-induced reactive oxygen species (ROS)-dependent catalytic therapy has been developed into a powerful strategy against bacterial wound infections. However, the limited endogenous supply or instability of HO, the reliance on external stimuli for the generation of ROS, and the highly expressed glutathione (GSH) level make it a challenge to achieve high-performance therapeutic efficiency. In this work, a facile therapeutic strategy against bacterial infections with pristine CuFe layered double hydroxide (LDH) as the self-cascade nanoreactor is proposed without modification or additional energy input.

Size transformation of Au nanoclusters for enhanced photocatalytic hydrogen generation: Interaction behavior at nanocluster/semiconductor interface.

Recently, atomically precise metal nanoclusters (NCs) become a new class of photosensitizer for light energy conversion in metal-cluster-sensitized semiconductor (MCSS) system. However, fundamental understanding for the suitable combination of NCs and semiconductor is still unclear. Aside from aspects of light harvesting, energy level alignment and catalytic activity, interfacial interaction behavior at NCs/semiconductor interface is also crucial due to its important influence in charge transportation.

NoiseMol: A noise-robusted data augmentation via perturbing noise for molecular property prediction.

Simplified Molecular-Input Line-Entry System (SMILES) is one of a widely used molecular representation methods for molecular property prediction. We conjecture that all the characters in the SMILES string of a molecule are essential for making up the molecules, but most of them make little contribution to determining a particular property of the molecule. Therefore, we verified the conjecture in the pre-experiment.

A New Fluorescent Probe Tool: ERNathG.

β-d-Glucuronidase (GUS) plays a pivotal role in both clinical treatment assessment and environmental monitoring. Existing tools for GUS detection suffer from (1) poor continuity due to a gap between the optimal pH of the probes and the enzyme and (2) diffusion from the detection site due to lack of an anchoring structure. Here we report a novel GUS pH-matching and endoplasmic reticulum-anchoring strategy for GUS recognition.

Specific Nanodrug for Diabetic Chronic Wounds Based on Antioxidase-Mimicking MOF-818 Nanozymes.

Chronic wound is a common complication for diabetic patients, which entails substantial inconvenience, persistent pain, and significant economic burden to patients. However, current clinical treatments for diabetic chronic wounds remain unsatisfactory. A prolonged but ineffective inflammation phase in chronic wounds is the primary difference between diabetic chronic wounds and normal wounds.

MRI Assessment of the Relationship Between Cortical Morphological Features and Hemiparetic Motor-Related Outcomes in Chronic Subcortical Stroke Patients.

Background: It is unclear which cortical regions are specific to or commonly associated with the impairments of the upper/lower limbs and the activities of daily life (ADL) in stroke patients.

Purpose: To investigate the relationships between MRI-assessed surface-based morphometry (SBM) features and motor function as well as ADL in participants with chronic stroke.

Study Type: Prospective.

Altered cerebellar functional connectivity in chronic subcortical stroke patients.

Background: Previous studies demonstrated that cerebellar subregions are involved in different functions. Especially the cerebellar anterior lobe (CAL) and cerebellar posterior lobe (CPL) have been postulated to primarily account for sensorimotor and cognitive function, respectively. However, the functional connectivity (FC) alterations of CAL and CPL, and their relationships with behavior performance in chronic stroke participants are unclear so far.

Platinum-Gold Alloy Catalyzes the Aerobic Oxidation of Formic Acid for Hydrogen Peroxide Synthesis.

On-site hydrogen peroxide production through electrocatalytic and photocatalytic oxygen reduction reactions has recently attracted broad research interest. However, practical applications have thus far been plagued by the low activity and the requirement of complex equipment. Here, inspired by the process of biological hydrogen peroxide synthesis catalyzed by enzymes, we report a Pt-Au alloy to mimic the catalytic function of natural formate oxidase for hydrogen peroxide synthesis through aerobic oxidation of formic acid.

Early Expression of Tet1 and Tet2 in Mouse Zygotes Altered DNA Methylation Status and Affected Embryonic Development.

Ten-eleven translocation (Tet) dioxygenases can induce DNA demethylation by catalyzing 5-methylcytosine(5mC) to 5-hydroxymethylcytosine(5hmC), and play important roles during mammalian development. In mouse, Tet1 and Tet2 are not expressed in pronucleus-staged embryos and are not involved in the genomic demethylation of early zygotes. Here, we investigated the influence of Tet1 and Tet2 on methylation of parental genomes by ectopically expressing Tet1 and Tet2 in zygotes.