Distribution and extended triage of high-risk human papillomavirus genotyping based on vaginal self-sampling.

Background: To investigate the overall and type-specific prevalence of high-risk human papillomavirus (HR-HPV) infection in China across different age groups and regions and to evaluate the triage of HPV-positive cases.

Methods: A prospective cross-sectional study recruited a total of 20 103 women aged 30-59 years from 13 provinces in China. Self-collected vaginal samples were tested for 14 HR-HPV types using next-generation sequencing or multiplex real-time PCR assays.

In vivo and in vitro protective effect of lutein against inflammatory response in lipopolysaccharide-induced broilers.

Lutein, a naturally occurring xanthophyll carotenoid abundant in leafy green vegetables, is known for its antioxidative and anti-inflammatory effects. This research assessed how lutein modulates intestinal inflammation triggered by lipopolysaccharide (LPS) exposure in vivo and in vitro. In vivo trials involved 240 male yellow-feathered broilers (1-day-old) divided into four groups: control (basal diet), lutein-supplemented (40 mg/kg), LPS-challenged (1 mg/kg), and lutein+LPS.

Co-cultivation of Lactobacillus acidophilus and Bacillus subtilis mediates the gut-muscle axis affecting pork quality and flavor.

Background: Pork quality and flavor are critical determinants of consumer preference, yet the role of gut microbiota in shaping meat characteristics remains underexplored. In this study, we investigated how a probiotic consortium (FAM: Lactobacillus acidophilus and Bacillus subtilis) modulates the gut-muscle axis to enhance pork flavor.

Results: In finishing pigs, FAM supplementation significantly increased flavor-associated nucleotides and umami-enhancing amino acids in longissimus dorsi muscle.

Fine-mapping of , a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew.

Introduction: Common wheat is a leading global food crop that impacts food security. Wheat powdery mildew (PM), caused by f. sp.

Health effects of astaxanthin in the intestinal tract of yellow-feathered broilers.

Astaxanthin (AST), a keto carotenoid, is widely recognized for its antioxidant, anti-inflammatory, and apoptosis-regulating properties, but its effects on intestinal health have not been elucidated. Therefore, this experiment aimed to investigate the effects of astaxanthin on intestinal morphology, barrier function, mucosal immunity, and cecal flora in yellow-feathered broilers, and to explore the potential mechanisms. A total of 288 male yellow-feathered broilers (1-day-old) were randomly allocated to four groups with six replicates of 12 birds each.

Supplementation with probiotics co-cultivation improves the reproductive performance in a sow-piglet model by mother-infant microbiota transmission and placental mTOR signaling.

Maternal nutritional supplementation has a profound effect on the growth and development of offspring. FAM is produced by co-cultivation of Lactobacillus acidophilus and Bacillus subtilis and has been demonstrated to potentially alleviate diarrhea, improve growth performance and the intestinal barrier integrity of weaned piglets. This study aimed to explore how maternal FAM improves the reproductive performance through mother-infant microbiota, colostrum and placenta.

Dietary Supplementation of Mixed Organic Acids Improves Growth Performance, Immunity, and Antioxidant Capacity and Maintains the Intestinal Barrier of Ira Rabbits.

The aim of this study was to investigate the effects of mixed organic acids (MOAs) on growth performance, immunity, antioxidants, intestinal digestion, and barrier function in Ira rabbits. A total of 192 weaned male Ira rabbits at 35 days of age were randomly assigned to four groups with six replicates of eight rabbits each. The rabbits in the control group (CON) were fed a basal diet, and the antibiotic group (SAL) was fed a basal diet supplemented with 60 mg/kg salinomycin.

Identifying changes in flood characteristics and their causes from an event-based perspective in the Central Taihu Basin.

Increasing rainstorms induced by climate change and modification in the land surface due to urbanization have greatly altered floods at different spatio-temporal scales. However, investigating flood events in urbanized plains is challenging as anthropogenic behaviors can change river flow without rainfall. In addition, while the frequency and magnitude of floods have been well examined, knowledge about variations in the rate of flood change is still limited.

Network structure and stability of the river connectivity in a rapidly urbanizing region.

River connectivity, which is key to the function of the river network, is deteriorating in highly urbanized areas. While previous studies have identified changes in the structure and connectivity of river networks, few studies have described the network structure of river connectivity and revealed the effects of urbanization on network structure. To this end, we detected the network structure and stability of river connectivity from a novel perspective of complex network theory.

The anti-inflammatory effect of lutein in broilers is mediated by regulating Toll-like receptor 4/myeloid-differentiation-factor 88 signaling pathway.

The anti-inflammatory role of lutein has been widely recognized, however, the underlying mechanism is still not fully elucidated. Hence, the effects of lutein on the intestinal health and growth performance of broilers and the action of mechanism were investigated. 288 male yellow-feathered broilers (1-day old) were randomly allocated to 3 treatment groups with 8 replicates of 12 birds each, and the control group was fed a broken rice-soybean basal diet, while the test groups were fed a basal diet added with 20 mg/kg and 40 mg/kg of lutein (LU20, LU40), respectively.

Analysis on the Effect of Radiofrequency Ablation and Electrocautery in the Treatment of Vaginal Intraepithelial Neoplasia.

Objective: This research intends to investigate the clinical efficacy of radiofrequency ablation and electrocautery in treating grade I or II vaginal intraepithelial neoplasia (VaIN).

Methods: This is a single-center retrospective study, which collected the clinical data of 100 patients with VaIN diagnosed by colposcopy and pathological biopsy in the Gynecology and Cervical Center of Xiangzhu Branch of the Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region between January 2020 and June 2021. Patients were divided into the study group (radiofrequency ablation treatment) and the control group (electrocautery) according to differences in treatment approaches.

A New Electron Shuttling Pathway Mediated by Lipophilic Phenoxazine via the Interaction with Periplasmic and Inner Membrane Proteins of MR-1.

Although it has been established that electron mediators substantially promote extracellular electron transfer (EET), electron shuttling pathways are not fully understood. Here, a new electron shuttling pathway was found in the EET process by MR-1 with resazurin, a lipophilic electron mediator. With resazurin, the genes encoding outer-membrane cytochromes ( and ) were downregulated.

Effectiveness and feasibility of self-sampling for human papillomavirus testing for internet-based cervical cancer screening.

Objectives: Worldwide, around 18.2% of cervical cancer occurred in China, mainly because of lower screening coverage and screening quality in regional disparities. To assess self-sampling for human papillomavirus (HPV) testing, combined with the internet, as a primary cervical cancer screening (CCS) method in low-resource settings, and to establish an internet-based self-sampling CCS-management model.

Marker-Independent Food Identification Enabled by Combing Machine Learning Algorithms with Comprehensive GC × GC/TOF-MS.

Reliable methods are always greatly desired for the practice of food inspection. Currently, most food inspection techniques are mainly dependent on the identification of special components, which neglect the combination effects of different components and often lead to biased results. By using Chinese liquors as an example, we developed a new food identification method based on the combination of machine learning with GC × GC/TOF-MS.

Spatial dependence of floods shaped by extreme rainfall under the influence of urbanization.

Weather system are spatially and temporally dependent, and these dependencies can result in flood events with similar behaviors. While it is well known that urbanization profoundly impacts the flood generation process, much less is known about the impacts of urbanization on the spatial dependence of floods, a major determinant of flood risk severity. To this end, a scheme was proposed to detect the flood dependence variations influenced by urbanization.

A powerful tool for near-infrared spectroscopy: Synergy adaptive moving window algorithm based on the immune support vector machine.

Traditional trial-and-error methods are time-consuming and inefficient, especially very unfriendly to inexperienced analysts, and are sometimes still used to select preprocessing methods or wavelength variables in near-infrared spectroscopy (NIR). To deal with this problem, a new optimization algorithm called synergy adaptive moving window algorithm based on the immune support vector machine (SA-MW-ISVM) is proposed in this paper. Following the principle of SA-MW-ISVM, the original problem of calibration model optimization is transformed into a mathematical optimization problem that can be processed by the proposed immune support vector machine regression algorithm.

A-type FHFs mediate resurgent currents through TTX-resistant voltage-gated sodium channels.

Resurgent currents () produced by voltage-gated sodium channels are required for many neurons to maintain high-frequency firing and contribute to neuronal hyperexcitability and disease pathophysiology. Here, we show, for the first time, that can be reconstituted in a heterologous system by coexpression of sodium channel α-subunits and A-type fibroblast growth factor homologous factors (FHFs). Specifically, A-type FHFs induces from Nav1.

Distinct biofilm formation regulated by different culture media: Implications to electricity generation.

Biofilm of Shewanella oneidensis MR-1 is extensively studied as it can transform organic compounds directly into electricity. Although revealing the biofilm regulation mechanism is crucial for enhancing bio-current, studies regarding the mechanism by which the culture condition affects biofilm formation are still lacking. The biofilm formation of S.

[Analysis of Rainfall Runoff Pollution and Pollution Load Estimation for Urban Communities in a Highly Urbanized Region].

The Taihu Lake plain is a highly urbanized region in China with many water-related environmental problems. Although point-source pollution has been effectively controlled by government legislation, urban surface runoff pollution is still a major issue. Different types of urban communities were selected for rainfall runoff experiments.

Fowl Adenovirus Serotype 4 Influences Arginine Metabolism to Benefit Replication.

Hydropericardium syndrome (HPS) is caused by fowl adenovirus serotype 4 (FAdV-4). HPS has caused outbreaks in Chinese populations of broiler chickens since 2015. However, little is known about the molecular mechanisms underlying HPS.

Repurposing Approved Drugs as Inhibitors of K7.1 and Na1.8 to Treat Pitt Hopkins Syndrome.

Purpose: Pitt Hopkins Syndrome (PTHS) is a rare genetic disorder caused by mutations of a specific gene, transcription factor 4 (TCF4), located on chromosome 18. PTHS results in individuals that have moderate to severe intellectual disability, with most exhibiting psychomotor delay. PTHS also exhibits features of autistic spectrum disorders, which are characterized by the impaired ability to communicate and socialize.

Discovery of Clinical Candidate 4-[2-(5-Amino-1H-pyrazol-4-yl)-4-chlorophenoxy]-5-chloro-2-fluoro-N-1,3-thiazol-4-ylbenzenesulfonamide (PF-05089771): Design and Optimization of Diaryl Ether Aryl Sulfonamides as Selective Inhibitors of Na1.7.

A series of acidic diaryl ether heterocyclic sulfonamides that are potent and subtype selective Na1.7 inhibitors is described. Optimization of early lead matter focused on removal of structural alerts, improving metabolic stability and reducing cytochrome P450 inhibition driven drug-drug interaction concerns to deliver the desired balance of preclinical in vitro properties.